Dong Chen¹, Xiang Wang^1,*, Fuzhou Tang², Yajin Zhao¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 150-150, 2019, DOI:10.32604/mcb.2019.07071

Abstract Deformability is a fundamental property of the cells and tissues of living organisms, which is commonly detected to indicate the state of the cells. And the cell deformability usually depends on the methods that we used, which is easy to be confused. The present research is designed to explore the energy characteristic of red blood cell deformability, based on a quantitative analysis of extending-retracting curves acquired from atomic force microscopy. ATP-depleted red blood cells are prepared by treatment with free-glucose Ringer solution. Our results clearly show that the Youngs’ modulus of erythrocyte is closely depended on the concentration of intracellular… More >

Stamatia Pagoulatou^1,*, Mauro Ferraro¹, Bram Trachet^1,2, Georgios Rovas¹, Vasiliki Bikia¹, Dionysios Adamopoulos³, Lindsey Crowe³, Jean-Paul Vallée³, Nikolaos Stergiopulos¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 148-149, 2019, DOI:10.32604/mcb.2019.07346

Abstract This article has no abstract. More >

Ying Li¹, Bin Xue², Wenxu Sun², Junhua Wu², Wenting Yu², Meng Qin², Wei Wang², Yi Cao^2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 147-147, 2019, DOI:10.32604/mcb.2019.07027

Abstract A key challenge in biomaterials research is to produce synthetic hydrogels that can replicate the diverse mechanical properties of the naturally occurring tissues for various biomedical applications, including tissue engineering, stem cell and cancer research, cell therapy, and immunomodulation. However, currently, the methods that can be used to control the mechanical properties of hydrogels are very limited and are mainly focused only on the elasticity of hydrogels. In this work, combining single molecule force spectroscopy, protein engineering and theoretical modeling, we show that synthetic protein hydrogels with predictable mechanical properties can be rationally designed using protein building blocks with known… More >

Qi Dang¹, Chun-Gong Li¹, Xin-Xin Jin¹, Ya-Jin Zhao¹, Xiang Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 146-146, 2019, DOI:10.32604/mcb.2019.07074

Abstract Endotoxin is a a very powerful and toxic inflammatory stimulator usually leading to the sepsis occurred. In order to remove endotoxin better through hemoperfusion, it is a pretty choice to increase the length of molecular linker on adsorbents. In this study, we chose the heparin as a molecular linker because of its being anticoagulant linear polysaccharide. Heparin as a linker was covalently immobilized on the chloromethylated polystyrene microspheres (Ps) and then connected with L-phenylalanine (Phe) forming the Ps-Hep-Phe structure to adsorbed endotoxin better. The property of microspheres was characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, zeta potential and… More >

Zongkun Hou¹, Shilei Hao¹, Bochu Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 145-145, 2019, DOI:10.32604/mcb.2019.07077

Abstract An expansion of the cerebral neocortex is thought to be the foundation for the unique intellectual abilities of humans. The cortical folding has been implicated in neurodevelopmental disorders and yet its origins remain unknown. In vitro culture of 3D cerebral organoids from human pluripotent stem cells has been used to study the fundamental mechanisms of mammalian neurodevelopment and characteristics of human brain development. Here, we explores the mechanism of boundary limitation on the formation of gyri and sulci through the self-organization of human brain organoids. The structure of cerebral organoids was analyzed by morphology observation, pathology and immunofluorescence. The different… More >

Danyang Yue¹, Yijuan Fan¹, Juan Lu¹, Mengxue Zhang¹, Jin Zhou¹, Yuying Bai¹, Jun Pan^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 144-144, 2019, DOI:10.32604/mcb.2019.07084

Abstract Mesenchymal Stem Cells (MSCs) are recruited to the musculoskeletal system following trauma [1] or chemicals stimulation [2]. The regulation of their differentiation into either bone or cartilage cells is a key question. The fluid shear stress (FSS) is of pivotal importance to the development, function and even the repair of all tissues in the musculoskeletal system [3]. We previously found that MSCs are sensitive enough to distinguish a slight change of FSS stimulation during their differentiation commitment to bone or cartilage cells, and the internal mechanisms. In detail, MSCs were exposed to laminar FSS linearly increased from 0 to 10… More >

Yuhua Gong¹, Shilei Hao^1,*, Bochu Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 143-143, 2019, DOI:10.32604/mcb.2019.07095

Abstract The mechanical response of brain tissue closely relates to cerebral blood flow and brain diseases. During intracerebral haemorrhage (ICH), a mass effect occurs during the initial bleeding. As the hematoma increases, the haematoma mass effect continues to squeeze the brain tissue mechanically, which can even lead to the formation of fatal cerebral hernia. However, fewer studies have focused on the brain damage mechanisms and treatment approaches associated with mass effects compared to the secondary brain injuries after ICH, which may be a result of the absence of acceptable animal models mimicking a mass effect. Thus, a thermo-sensitive poly (N-isopropylacrylamide) (PNIPAM)… More >

Jianxiong Xu¹, Lu Wang¹, Jinxuan Wang¹, Juhui Qiu^1,*, Guixue Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 142-142, 2019, DOI:10.32604/mcb.2019.07273

Abstract Endothelial cell injured or dysfunction, which results lipid deposition and inflammation, is the key point to exacerbate the process of atherosclerosis [1, 2]. Meanwhile oscillatory shear stress is a key factor that results cell dysfunction in vascular disease [3, 4]. Previous research reported that P2Y12 plays a critical role in the development of atherosclerotic lesion through promoting smooth muscle cells migration [5]. As well P2Y12 stimulated the internalization and transendothelial transport of high density lipid. However, whether the P2Y12 induce atherosclerosis through endothelial cell remain elusive. In this study we firstly found P2Y12 were expressed in endothelial cells of atheroprone… More >

Yang Wang¹, Shuang Ge¹, Junyang Huang¹, Ruolin Du¹, Tieying Yin¹, Guixue Wang^1,*, Yazhou Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 140-141, 2019, DOI:10.32604/mcb.2019.07300

Abstract Zonula occludens-1 (ZO-1) is a peripheral membrane protein belongs to the family of zona occludens proteins and plays an important role as a scaffold protein which cross-links and anchors tight junction (TJ) strand proteins, within the lipid bilayer, to the actin cytoskeleton^[1-2]. Stent implantation is the most effective method in the treatment of cardiovascular disease which always destroy junctions of endothelial cells, the functions of the tight junction were also affected. However, the role of ZO-1 before and after stent implantation has not been fully understood. In this study, the expression of ZO-1 were analyzed by qPCR, western blot and… More >

Junyang Huang¹, Shuang Ge¹, Yang Wang¹, Ruolin Du¹, Yazhou Wang¹, Tieying Yin¹, Guixue Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 138-139, 2019, DOI:10.32604/mcb.2019.07305

Abstract Tight junctions are the most apical intercellular junctions of the lateral membrane in endothelial cells, regulating the paracellular material and energy exchange and maintain plasma membrane polarity. Occludin protein is one of the important proteins involved in endothelial tight junctions, and also closely related to the occurrence of atherosclerosis. Therefore, the study of occludin is valuable ^[1]. With the implantation of coronary stents, the integrity of the vascular endothelium is damaged and the local mechanical environment at the stent segment was changed ^[2]. The present study tried to explore the impact of mechanical stimulation after stent implantation on the expression… More >