Xinyi Zhang, Zimeng Yu, Pingfang Yang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1665-1679, 2023, DOI:10.32604/phyton.2023.029482

Abstract Sacred lotus (Nelumbo nucifera) is a typical aquatic plant, belonging to basal eudicot plant, which is ideal for genome and genetic evolutionary study. Understanding lotus gene diversity is important for the study of molecular genetics and breeding. In this research, public RNA-seq data and the annotated reference genome were used to identify the genes in lotus. A total of 26,819 consensus and 1,081 novel genes were identified. Meanwhile, a comprehensive analysis of gene alternative splicing events was conducted, and a total of 19,983 “internal” alternative splicing (AS) events and 14,070 “complete” AS events were detected in 5,878 and 5,881 multi-exon… More >

Adalberto Benavides-Mendoza¹, Rebeca Betancourt-Galindo², Nazario Francisco-Francisco^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1831-1840, 2023, DOI:10.32604/phyton.2023.028085

Abstract

Micronutrient nanoparticles (NPs) are currently an option for chemical fertilization and biostimulation in crops. However, there is little information on the phytotoxic or biostimulatory effects of NPs at low concentrations of some elements, such as Zn. In this study, the effect of low concentrations of Zn oxide (ZnO) NPs on germination, growth variables, and nutritional attributes of lettuce (Lactuca sativa L.) was evaluated in comparison to Zn sulfate. Romaine lettuce seeds were treated with ZnSO₄^-- × 7H₂O and ZnO NPs at Zn molar concentrations of 1 × 10⁻³, 5 × 10⁻³, 1 × 10⁻⁴, 5 × 10⁻⁴, 1… More >

Mohammad Reza Boorboori, Husen Qiu, Jieyun Liu, Haiyang Zhang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1873-1886, 2023, DOI:10.32604/phyton.2023.027924

Abstract Rice is an essential part of the human diet in most parts of the world; On the other hand, the industrialization of societies has led to pollution of the environment, including heavy metal contamination of soil and water, which negatively affects rice production and quality. Therefore, finding ways to increase the yield and quality of this strategic crop seems essential. Several studies have been conducted in recent decades to find effective and inexpensive solutions to reduce the adverse effects of heavy metals in rice fields. Due to the negative effect of cadmium pollution on rice quality and yield, the current… More >

Xianrong Fu, Aiping Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen^*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1681-1694, 2023, DOI:10.32604/phyton.2023.027870

Abstract Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The DWF4 gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identified a homologous gene of DWF4 in chrysanthemum, CmDWF4. This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of CmDWF4 in chrysanthemum was found to significantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosylase/hydrolase (XTH) family encoding genes associated with… More >

Syed Ali Zafar^1,*, Muhammad Aslam², Haroon Zaman Khan³, Sehrish Sarwar¹, Rao Saad Rehman⁴, Mariam Hassan¹, Ramala Masood Ahmad², Rafaqat A. Gill⁵, Basharat Ali⁶, Ibrahim Al-Ashkar⁷, Abdullah Ibrahim⁷, Md Atikur Rahman⁸, Ayman El Sabagh^9,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1887-1906, 2023, DOI:10.32604/phyton.2023.027679

Abstract The availability of favorable genetic diversity is a thriving vitality for the success of a breeding program. It provides a firm basis of selecting superior breeding lines for the development of high yielding crop genotypes. In this context, present investigation aimed to generate information on genetic divergence and character association in a diversity panel of 123 local and exotic soybean genotypes. Analysis of variance revealed significant response of the evaluated genotypes based on studied attributes. It depicted the probability of selecting desirable soybean genotypes by focusing on character association studies and genetic diversity analysis. Correlation analysis revealed that seed yield… More >

Yeyao Du^1,#, Ye Zhang^2,#, Yang Song¹, Zhuo Zhang¹, Sujie Fan¹, Hanzhu Zhang¹, Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1907-1920, 2023, DOI:10.32604/phyton.2023.027607

Abstract Soybean (Glycine max (Linn.) Merr.) annual leguminous crop is cultivated all over the world. The occurrence of diseases has a great impact on the yield and quality of soybean. In this study, based on the RNA-seq of soybean variety M18, a complete CDS (Coding sequence) GmPR1L of the pathogenesis-related protein 1 family was obtained, which has the ability to resist fungal diseases. The overexpression vector and interference expression vector were transferred into tobacco NC89, and the resistance of transgenic tobacco (Nicotiana tabacum L.) to Botrytis cinerea infection was identified. The results show that: Compared with the control, the activities of… More >

Minmin Xiao¹, Moxiang Cheng¹, Shuangquan Xie¹, Xiushuang Wang¹, Xingming Hao², Li Zhuang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1841-1857, 2023, DOI:10.32604/phyton.2023.027369

Abstract To clarify the characteristics of photoinhibition and the primary defense mechanisms of ephemeral plant leaves against photodestruction under high temperature stress, inhibitors and the technology to determine chlorophyll fluorescence were used to explore the protective effects of D1 protein turnover and the lutein cycle in the high temperature stress of the leaves of three ephemeral plants. The results showed that the maximum light conversion efficiency (Fv/Fm) of the ephemeral plant leaves decreased, and the initial fluorescence (Fo) increased under 35°C ± 1°C heat stress for 1–4 h or on sunny days in the summer. Both Fv/Fm and Fo could be… More >

Xiaotian Liang^1,#, Qi Wen^1,#, Rui Luo¹, Yanxin Zhang¹, Mingda Yin¹, Yanpeng Wen¹, Xuemei Hu¹, Zhiyan Wang¹, Yumiao Huo¹, Fenglan Huang^{1,2,3,4,5,6,*}

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1765-1792, 2023, DOI:10.32604/phyton.2023.026935

Abstract Castor bean (Ricinus communis L.), is one of the top 10 oilseed crops in the world and, therefore, of high economic value. Hybridization is one of the most effective ways to breed new varieties with high yield, high oil content, and better stress resistance. Therefore, prediction of desired traits in castor hybrid offspring is particularly important. In this study, proteomic analysis was performed to identify differentially expressed proteins (DEPs) in seeds between castor hybrid offspring and their female (Lm female line aLmAB₂) and male parents (CSR·181). Among the DEPs upregulated in the seeds of hybrid offspring, the majority were related… More >

Yanyan Pu^1,#, Liwen Wang^2,#, Leilei Li³, Yujun Si⁴, Shubin Xie², Yunzhe Cong¹, Dong Wang¹, Yongchao Gong¹, Rumei Tian¹, Xue Chen³, Xiaoyan Zhang¹, Min Liu¹, Hanfeng Ding^1,3,*, Nana Li^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822

Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total DEGs, 129 genes were investigated… More >

Chen Liu^1,2 , Xiaowei Tang^1,2, Xiaohui Qu^1,2 , Fadi Chen¹, Jiafu Jiang¹ and Sumei Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1391-1404, 2023, DOI:10.32604/phyton.2023.027231

Abstract Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis.… More >