F.L. RICCILLO, M.I. BRACAMONTE, G.M. CÓNSOLE, C.L.A. GÓMEZ DUMM

BIOCELL, Vol.28, No.2, pp. 127-134, 2004, DOI:10.32604/biocell.2004.28.127

Abstract Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen).… More >

S.K. VILA*, H.Y. REY, L.A. MROGINSKI

BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l^-1 benzylaminopurine (BAP) + 0.1mg.l^-1 kinetin (KIN) + 3 mg.l^-1 adenine sulphate (ADS).
The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the genotypes… More >

MARÍA DE LOS ANGELES ALVAREZ, VALERIA E. P. ZARELLI, NORA B.PAPPANO, NORA B. DEBATTISTA

BIOCELL, Vol.28, No.1, pp. 31-34, 2004, DOI:10.32604/biocell.2004.28.031

Abstract In previous work the bacteriostatic action of trihydroxylated chalcones against Staphylococcus aureus ATCC 25 923 was investigated. In this work the action of 2´, 4´, 2-(OH)₃ -chalcone, 2´, 4´, 3-(OH)_3^- chalcone and 2´, 4´, 4-(OH)₃ –chalcone against Escherichia coli ATCC 25 922 was evaluated. Growth kinetic curves of E.coli were made in nutritive broth added with increasing drug concentrations. The specific growth rates of the microorganisms were calculated by a kinetic turbidimetric method, which was previously probed and the minimal inhibitory concentrations (MIC´s) were evaluated by a mechanism of action proposed. The MICs of 2´, 4´, 3-(OH)₃ -chalcone and 2´,… More >

RENATO SIMÕES CORDEIRO*, WELLERSON RODRIGO SCARANO*, REJANE MAIRA GÓES**, SEBASTIÃO ROBERTO TABOGA**

BIOCELL, Vol.28, No.1, pp. 21-30, 2004, DOI:10.32604/biocell.2004.28.021

Abstract The flutamide antiandrogenic effects on the Guinea pig male prostate morphology in puberal, post-puberal and adult ages were evaluated in the present study. Daily-treated group animals received flutamide subcutaneous injection at a dose of 10 mg/Kg body weight for 10 days. The control group animals received a pharmacological vehicle under the same conditions. The lateral prostate was removed, fixed and processed for light and transmission electron microscopy. The results revealed an increase of the acinus diameter in the treated puberal animals and straitness in the stromal compartment around the acini. The epithelial cells exhibited cubic phenotype. In the post-puberal and… More >

R. MEDINA, M. FALOCI, M.A. MARASSI, AND L.A. MROGINSKI.

BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l^-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l^-1.… More >

M. ISOLDE RUDOLPH¹, I. GINA ROJAS², ALICIA B. PENISSI³

BIOCELL, Vol.28, No.1, pp. 1-11, 2004, DOI:10.32604/biocell.2004.28.001

Abstract Birth is the result of complex, well-defined, and coordinated events, that are tightly regulated by endocrine, nervous, and immune responses, and take place primarily in the female reproductive tract. Various mechanisms and mediators involved in pregnancy, labor, and delivery, are highly conserved among different mammalian species and mast cells emerge as potential and crucial participants in these processes, as it is discussed in this review. More >

OFELIA ACOSTA DE PÉREZ¹, LAURA LEIVA DE VILA², MARÍA ELISA PEICHOTO², SILVANA MARUÑAK³, RAQUEL RUÍZ³, PAMELA TEIBLER³, CAROLINA GAY², LAURA REY⁴

BIOCELL, Vol.27, No.3, pp. 363-370, 2003, DOI:10.32604/biocell.2003.27.363

Abstract Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 µl of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 µl of PBS. Two hours after injection mice were killed… More >

ADELINA FERREIRA, HEIDI DOLDER

BIOCELL, Vol.27, No.3, pp. 353-362, 2003, DOI:10.32604/biocell.2003.27.353

Abstract Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the proacrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation.… More >

LUZMARINA HERNANDES*, LUCIENI CRISTINA MARQUES DA SILVA PEREIRA*, ELIANA PARISI ALVARES**

BIOCELL, Vol.27, No.3, pp. 347-351, 2003, DOI:10.32604/biocell.2003.27.347

Abstract The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied… More >

ALBERTO J. SOLARI*, MONICA I. RAHN*, ALICIA SAURA**, HUGO D. LUJAN**

BIOCELL, Vol.27, No.3, pp. 329-346, 2003, DOI:10.32604/biocell.2003.27.329

Abstract The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs… More >