CARMINDA DA CRUZ-LANDIM*, FÁBIO CAMARGO ABDALLA*, MARIA ALICE DA CRUZ-HÖFLING**

BIOCELL, Vol.27, No.3, pp. 319-328, 2003, DOI:10.32604/biocell.2003.27.319

Abstract The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type… More >

LUIS A. CARO¹, NATALIA SANTECCHIA¹, PABLO A. MARINANGELI¹, NÉSTOR R. CURVETTO^1,2, LUIS F. HERNÁNDEZ^1,3

BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311

Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L^-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >

JUAN CARLOS CAVICCHIA^1*, MABEL FÓSCOLO¹ , JORGE IBAÑEZ¹, CHRISTOPHER LILLIG², FRANCISCO CAPANI³

BIOCELL, Vol.35, No.3, pp. 81-90, 2011, DOI:10.32604/biocell.2011.35.081

Abstract Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages… More >

QINGXIN LIU^1§ , XIANGGUI CHEN^2§, GUOLIN YANG¹ , XUEWEN MIN³ , AND MAOXIAN DENG^1,*

BIOCELL, Vol.35, No.3, pp. 71-80, 2011, DOI:10.32604/biocell.2011.35.071

Abstract Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream… More >

VIJAY L. KUMAR^* AND BISWA M. PADHY

BIOCELL, Vol.35, No.3, pp. 63-70, 2011, DOI:10.32604/biocell.2011.35.063

Abstract Calotropis species have been used in the traditional medicinal system for the treatment of diseases of the liver and abdomen. In view of the antioxidant and anti-hyperglycemic properties of an aqueous suspension obtained from the dried latex of Calotropis procera, the present study was carried out to evaluate its efficacy in affording protection against alloxan induced changes in rat kidney. A single intraperitoneal injection of alloxan (150 mg/kg) in rats produced hyperglycemia within 3 days and altered kidney functions over a period of 90 days. Daily oral administration of the aqueous suspension (100 and 400 mg/kg) in diabetic rats produced… More >

ALICIA KOHLI¹ , STELLA M. PEZZOTTO² , GRACIELA GARCIA³ , LEONOR C. POLETTO².

BIOCELL, Vol.35, No.2, pp. 51-58, 2011, DOI:10.32604/biocell.2011.35.051

Abstract The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed… More >

YOUNG HWA LEE¹, HEE SAM NA¹ , SO YEON JEONG¹, SUNG HEE JEONG² , HAE RYOUN PARK³ , JIN CHUNG^1*

BIOCELL, Vol.35, No.2, pp. 43-50, 2011, DOI:10.32604/biocell.2011.35.043

Abstract MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA microarray was carried out and the expression of miRNAs was confirmed by real-time PCR.… More >

MARÍA B. ESPINOSA² , NICOLÁS A. FRAUNHOFFER¹ , NOELIA P. LEOPARDO¹ , ALFREDO D. VITULLO² AND MIGUEL A. WILLIS¹

BIOCELL, Vol.35, No.2, pp. 37-42, 2011, DOI:10.32604/biocell.2011.35.037

Abstract Lagostomus maximus is a notable mammalian model for reproductive studies. Females have an extremely high ovulation rate, which is due to down-regulation of the follicular apoptosis pathway, which ensures a large pool of developing follicles. This large pool is supported by the convoluted anatomy of the mature ovary, whose germinal tissue is found in irregularly curved ridges throughout the cortex. Medullary tissue is restricted to a minimum. Lyso Tracker Red reconstruction under confocal laser scanning microscopy was used to recognize and measure all follicular stages from primordial to antral. Unlike most mammals in which early primordial follicles are just found… More >

G. P. CARDOZO¹ , E. V. SANTOS¹ , A. L. FACHIN¹, S. C. FRANÇA¹ , AND M. MARINS^1,2,*

BIOCELL, Vol.35, No.1, pp. 35-36, 2011, DOI:10.32604/biocell.2011.35.035

Abstract E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR. More >

MIRIAM TERUEL^1,2, JAQUELINE D’ERCOLE¹ AND RODOLFO CATALANO²

BIOCELL, Vol.35, No.1, pp. 29-34, 2011, DOI:10.32604/biocell.2011.35.029

Abstract Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Females were superovulated by intraperitoneal injection of 10 IU of eCG and 10 IU of hCG (48h later) and were paired with males of proven fertility. Albendazole sulphoxide (200 mg/kg) was orally administered by gavages at day 1, 2 or 3 of pregnancy; the control group received only the vehicle (carboxymethylcellulose). Females… More >