YUANNA DU^1,#, WENWEN GONG^2,#, JING LIANG^1,#, RUKUN ZANG¹, JUNJUN MOU¹

BIOCELL, Vol.46, No.5, pp. 1181-1188, 2022, DOI:10.32604/biocell.2022.016148

Abstract Endometrial cancer remains to be a major type of malignancy in threatening female life. Molecular insights in advancing our understanding of endometrial tumorigenesis are much needed. We here report that a less-studied protein Dihydropyrimidinase like 3 (DPYSL3) is a potent tumor suppressor. DPYSL3 is uniquely regulated by wild type p53 (wtp53), and its expression is at the highest level when cells carry wtp53 and are exposed to hypoxia. We reveal that wtp53 can bind DPYSL3 promoter to enhance DPYSL3 expression and in turn, the elevated DPYSL3 can restrain cancer cell proliferation and invasion in vitro and in vivo. Importantly, we… More >

YANG YANG, LIJUAN FU, CHUNMEI CHEN, MEIWEI HU

BIOCELL, Vol.46, No.5, pp. 1319-1328, 2022, DOI:10.32604/biocell.2022.015760

Abstract Aberrant expression of miRNAs is significantly correlated with the occurrence of immune thrombocytopenic purpura (ITP). The immune imbalance of M1/M2 macrophage contributes to the development of ITP. However, the role of miR-148b-3p in macrophage phenotype imbalance remains unknown in ITP. In this study, we aimed to explore whether miR-148b-3p inhibits M2 macrophage polarization in ITP and to investigate the underlying mechanism. Peripheral blood from 22 ITP patients were collected, and real-time PCR confirmed that miR-148b-3p was up-regulated and Western blot analyses detected the expression of SOCS3 was down-regulated. Subsequent dual-luciferase reporter gene assay indicated that miR-148b-3p could bind to SOCS3.… More >

YUNLONG ZHANG^1,#, JINGWEI ZHANG^2,#, YU ZHU¹, BIN CAI^1,*

BIOCELL, Vol.46, No.5, pp. 1329-1338, 2022, DOI:10.32604/biocell.2022.015592

Abstract Diabetic patients often exhibit delayed or incomplete progress in the healing of acute wounds, owing to poor blood perfusion. Platelet-rich plasma (PRP) has attracted much attention as a means to improve wound healing, because it contains high growth factor concentrations. However, the burst-like release of PRP growth factors results in a short half-life of these therapeutic proteins, thus greatly limiting the therapeutic effect. In this study, we prepared PRP from human umbilical cord blood and developed an in situ photocrosslinkable PRP hydrogel glue (HNPRP) by adding a photoresponsive hyaluronic acid (HA-NB) into PRP. The HNPRP hydrogel allowed for controlled release… More >

TAILIN WU^1,#; XIANG ZHOU^2,#; CANHUA YE¹; WENCAN LU¹; HAITAO LIN¹; YANZHE WEI¹; ZEKAI KE¹; ZHENGJI HUANG¹; JIANZHOU LUO¹; HUIREN TAO¹; CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.4, pp. 1123-1123, 2022, DOI:10.32604/biocell.2022.020679

Abstract This article has no abstract. More >

FEI CHENG^1,#, YIQIAN DING^2,#, QING XU³, WEI ZHANG³, YULAN ZHEN⁴, JING LIU⁵, SHICHENG LI¹, CHANG TU¹, GUOHUA LAI¹, JUN LAN^1,*, JINGFU CHEN^1,*

BIOCELL, Vol.46, No.4, pp. 1053-1066, 2022, DOI:10.32604/biocell.2021.012901

Abstract Previous reports have suggested that Ang-(1-7) may have a protective effect in endothelial cells against high glucose (HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling pathway. In this study, we have examined whether NF-κB-IL-1β and Heme oxygenase-1 (HO-1) pathways contribute to the protection of Ang-(1-7) against hyperglycemia-induced inflammation and oxidative stress in human umbilical vein endothelial cells (HUVECs). Our results indicate that time-varying exposures of HUVECs, from 1 h to 24 h, to high glucose concentrations result in an increased expression of phosphorylated (p)-p65 and HO-1 in a time-dependent manner. As an inhibitor of NF-κB, pyrrolidinedithiocarbamic… More >

MICHELE DI COSOLA^1,§; STEFANIA CANTORE^2,§,*; MARIO GIOSUÈ BALZANELLI³; CIRO GARGIULO ISACCO^2,3; KIEU CAO DIEM NGUYEN^2,3; RAJIV SAINI²; ANNARITA MALCANGI⁴; MARGHERITA TUMEDEI⁵; MARIATERESA AMBROSINO¹; ANTONIO MANCINI²; SALVATORE SCACCO⁶; RICCARDO NOCINI⁷; LUIGI SANTACROCE²; ANDREA BALLINI^8,9,#,*; EDOARDO BRAUNER^10,#

BIOCELL, Vol.46, No.4, pp. 923-929, 2022, DOI:10.32604/biocell.2022.018409

Abstract The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs), so called dental-derived stem cells (D-dSCs) with self-renewing and multilineage differentiation capabilities. D-dSCs properties involves a strong paracrine component resulting from the high levels of bioactive molecules they secrete in response to the local microenvironment. Altogether, this viewpoint develops a general picture of current innovative strategies to employ D-dSCs combined with biomaterials and bioactive factors for regenerative medicine purposes, and offers information regarding the available scientific data and possible applications. More >

KENJI TSUJI^*, SHINJI KITAMURA, JUN WADA

BIOCELL, Vol.46, No.4, pp. 899-902, 2022, DOI:10.32604/biocell.2022.018594

Abstract Mesenchymal stem cells (MSCs) have abilities to mediate tissue protection through mechanisms of anti-apoptosis, anti-oxidative stress and anti-fibrosis as well as tissue regeneration through mechanisms of cell proliferation, differentiation and angiogenesis. These effects by MSCs are mediated by a variety of factors, including growth factors, cytokines and extracellular vesicles (EVs). Among these factors, EVs, containing proteins, mRNA and microRNAs (miRNA), may carry their contents into distant tissues with high stability. Therefore, the treatment with MSC-derived EVs may be promising as ‘natural’ drug delivery systems (DDS). Especially, the treatment of MSC-derived EVs with the manipulation of specific miRNAs expression has been… More >

THAÍS CASAGRANDE PAIM, MÁRCIA ROSÂNGELA WINK^*

BIOCELL, Vol.46, No.4, pp. 913-922, 2022, DOI:10.32604/biocell.2022.018498

Abstract Mesenchymal stem cells (MSCs) play key roles in regenerative medicine by promoting tissue healing. MSCs can be isolated from different adult tissues and they are able to differentiate into several lineages. Due to their anti-inflammatory, angiogenic and immune-modulatory properties, MSCs are suitable for tissue engineering applications and, when associated with biomaterials, their benefits can be improved. Moreover, recently, MSCs have been studied for new clinical applications, such as in the treatment of patients with COVID-19. MSCs regenerative potential has been attributed to their secretome, which comprises extracellular matrix, soluble proteins and several elements, including the release of extracellular vesicles. Even… More >

PETRA KRAUS^1,*, ANKITA SAMANTA¹, SINA LUFKIN², THOMAS LUFKIN¹

BIOCELL, Vol.46, No.4, pp. 893-898, 2022, DOI:10.32604/biocell.2022.018432

Abstract Pain and lifestyle changes are common consequences of intervertebral disc degeneration (IVDD) and affect a large part of the aging population. The stemness of cells is exploited in the field of regenerative medicine as key to treat degenerative diseases. Transplanted cells however often face delivery and survival challenges, especially in tissues with a naturally harsh microniche environment such as the intervertebral disc. Recent interest in the secretome of stem cells, especially cargo protected from microniche-related decay as frequently present in degenerating tissues, provides new means of rejuvenating ailing cells and tissues. Exosomes, a type of extracellular vesicles with purposeful cargo… More >

CLAIRE ELIZABETH HILLS, ELEFTHERIOS SIAMANTOURAS, PAUL EDWARD SQUIRES^*

BIOCELL, Vol.46, No.4, pp. 937-940, 2022, DOI:10.32604/biocell.2022.018414

Abstract Changes in cell-cell and cell-substrate adhesion markers are increasingly used to characterize disease onset and progression. However, these relationships depend on both the biochemical and molecular association between cells and between cells and their extracellular matrix, as well as the biophysical and mechanical properties orchestrated by cytoskeletal, membrane and matrix components. To fully appreciate the role of cell adhesion when determining normal physiology and the impact of disease on cellular function, it is important to consider both biochemical and biophysical attributes of the system being investigated. In this short viewpoint we reflect on our experiences assessing cell-cell and/or cell-matrix interactions… More >