RUXIN ZHANG¹, CHENGGANG LI², RUOCHEN DU¹, YITONG YUAN¹, BICHUN ZHAO¹, YUJUAN ZHANG¹, CHUNFANG WANG^1,*

BIOCELL, Vol.46, No.3, pp. 583-592, 2022, DOI:10.32604/biocell.2022.017248

Abstract Extracellular β-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) are the pathological hallmarks of Alzheimer’s disease (AD). Studies have shown that aggregates of extracellular Aβ can induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors. Thus, modulation of Aβ might be a potential therapeutic strategy for modifying disease progression. Recently, a large number of reports have confirmed the beneficial effects of mesenchymal stem cells (MSCs) on AD. It is believed to reduce neuroinflammation, reduce Aβ amyloid deposits and NFTs, increase acetylcholine levels, promote neurogenesis, reduce neuronal damage, and improve working memory and cognition. In this review, we… More >

MARÍA G. FLORES-SÁNCHEZ^1,*, ROBERTO OLAYO², J. MORALES-CORONA², ATLÁNTIDA M. RAYA-RIVERA³, DIEGO R. ESQUILIANO-RENDÓN³

BIOCELL, Vol.46, No.3, pp. 829-836, 2022, DOI:10.32604/biocell.2022.016988

Abstract This article presents the construction of scaffolds composed of polylactic acid (PLA) with different concentrations of hydroxyapatite (HA) by electrospinning, which were superficially modified with polypyrrole (PPy/I) by plasma polymerization. A preliminary study was conducted of the biological and mechanical behavior of the scaffolds when they were implanted in the back of rabbits for 30 days; bone cells differentiated from mesenchymal stem cells (MSCs) were used. The bone cell and scaffold structures were characterized by histological, immunohistochemical, and mechanical stress tests. Hematoxylin–eosin staining showed good tissue conformation. The immunohistochemical tests highlighted the presence of the main bone tissue proteins, such… More >

WAN ZHANG^1,2,#, GUIYAN YANG^3,#, YUNLIN ZHAO¹, ZHENGGANG XU^1,2,3,*, HUANG HUIMIN¹, JIAKANG ZHOU¹

BIOCELL, Vol.46, No.3, pp. 803-819, 2022, DOI:10.32604/biocell.2022.016984

Abstract Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp to 162,594… More >

JIRUI WEN^1,#, WENCHAO WU^2,#, MIN TANG¹, MINGYUE BAO¹, XUELING HE³, XINGHONG YAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.3, pp. 655-665, 2022, DOI:10.32604/biocell.2022.016915

Abstract To dissect which subset of bone marrow monocyte is the major precursor of osteoclast, 3-month-old rat bone marrow was obtained for single-cell RNA sequencing. A total of 6091 cells were acquired for detailed analysis, with a median number of 1206 genes detected per cell and 17,959 genes detected in total. A total of 19 cell clusters were recognized, with the main lineages identified as B cells, Granulocytes, Monocytes, T cells, Erythrocytes and Macrophages. Monocytes were further divided into classical monocytes and non-classical monocytes. Compared with non-classical monocytes, classical monocytes highly expressed osteoclast differentiation related genes Mitf, Spi1, Fos and Csf1r.… More >

ABDELKADER HASSANI^1,2,3, SITI ASLINA HUSSAIN², MOTHANNA SADIQ AL-QUBAISI⁴, MOHAMED LAKHDER BELFAR³, HAKIM BELKHALFA⁵, HAMID HAMMAD ENEZEI⁶, HAMID ZENTOU², WISAM NABEEL IBRAHIM^7,8,*, ABD ALMONEM DOOLAANEA^1,*

BIOCELL, Vol.46, No.3, pp. 677-687, 2022, DOI:10.32604/biocell.2022.016848

Abstract The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin (Cur) through nanoformulation with gum Arabic (GA) as a coating agent through an efficient synthetic approach. The antioxidant properties of the developed nanoparticles (Cur/GANPs) were assessed through several in vitro assays, such as β-carotene bleaching activity, DPPH, and nitric oxide scavenging activities in addition to evaluating its inhibitory activity on angiotensin-converting enzyme (ACE). The cytotoxicity of Cur/GANPs was evaluated in vitro using different types of human cancer cells including breast cancer (MCF7, MDA-MB231), liver cancer (HepG2), and colon cancer (HT29) cells. The prepared particles… More >

MINGMING FANG^1,#, NING GE^2,#, JIANFANG LIU^3,*, YAYUN CUI^2,*

BIOCELL, Vol.46, No.3, pp. 711-720, 2022, DOI:10.32604/biocell.2022.016714

Abstract The resistance of cancer cells to the anti-cancer drugs is the most important reason that affecting the efficacy of the non-small cell lung cancer (NSCLC) chemotherapy; thus, to explore the underlying mechanism of drug resistance of NSCLC medications is urgently needed for improving the therapeutic efficacy of current anti-NSCLC chemotherapies. The aim of the present study is to explore the roles of exosomes in the chemosensitivity of A549 cells and the related mechanism. A549 cells and cisplatin resistant cell line A549/DDP derived exosomes were isolated, and the expressions of CXCR4 were compared. Then, after cisplatin treatment, A549 cells were treated… More >

RAGHDA A. SOLIMAN¹, RANA A. YOUNESS^1,2,*, TAMER M. MANIE³, EMAD KHALLAF⁴, MOHAMED EL-SHAZLY¹, MONA ABDELMOHSEN⁵, HEBA HANDOUSSA¹, MOHAMED Z. GAD^6,*

BIOCELL, Vol.46, No.3, pp. 769-783, 2022, DOI:10.32604/biocell.2022.016636

Abstract Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these cytokines’ levels via non-coding RNAs (ncRNAs) might alleviate the immune quiescent nature of TME at TNBC tumors. However, the extrapolation of ncRNAs as therapeutic tools is highly challenging. Therefore, disentanglement the nature for the isolation of natural compounds that could modulate the ncRNAs and their respective targets is an applicable translational… More >

XUE MEI LUO¹, SHAHANAVAJ KHAN^2,3,4,*, ABDUL MALIK², FAHAD M. ALDAKHEEL⁵, ANIS AHMAD CHAUDHARY⁶, SHOUKI BAZARBASHI⁷, FATEMEH TABATABAIE⁸

BIOCELL, Vol.46, No.3, pp. 759-767, 2022, DOI:10.32604/biocell.2022.016586

Abstract Despite the multiple systematic reviews and meta-analyses accumulating evidence on the preventive effect of calcium supplementation for colorectal cancer, most of the associated adverse effects are not systematically analyzed. The aim of the study is evaluating adverse events associated with calcium supplementation for colorectal cancer prevention through a systematic meta-analysis. We searched Medline, PubMed Central, EMBASE (Excerpta Medica database), Scopus, Cochrane Central Register of Controlled Trials, and Web of Science published in English from database inception up to 31 July 2019. In the current systematic meta-analysis, we included human studies (including cohort studies, clinical trials, case-control studies) on supplementation of… More >

SHUTING GU^1,2,#, JINGYI QIN^3,#, SAINAN GAO¹, ZHEN WANG^1,4, QI MENG^1,4, YAN LI^1,4, BING LU⁵, SONGLIN ZHOU^1,2,*, YUNZHAO XU^1,6,*

BIOCELL, Vol.46, No.3, pp. 689-697, 2022, DOI:10.32604/biocell.2022.016225

Abstract Recently, abnormal expression of KIAA1199 has been detected in Epithelial Ovarian Cancer (EOC). However, the underlined anti-ovarian cancer mechanism of KIAA1199 remains to be enlightened. In our study, we performed to elucidate the effects of KIAA1199 on the advanced biological behavior of EOC cells through activation of the IL-6/STAT3 pathway. Confirmed by immunohistochemistry, KIAA1199 was highly expressed in ovarian borderline and malignant epithelial tumors. A retrospective analysis found that EOC patients with low expression of KIAA1199 had a significantly higher 5-year survival rate than those with high expression. Mechanistically, IL-6 was used to stimulate EOC cells, and the expression of… More >

XUE JIANG^1,2, HONG ZHANG^1,2,*

BIOCELL, Vol.46, No.3, pp. 785-794, 2022, DOI:10.32604/biocell.2022.016167

Abstract Posterior capsular opacification (PCO) is linked to the pathological process of lens epithelial cells, which includes proliferation, migration, and epithelial-mesenchymal transition (EMT). Our goal was to investigate whether long noncoding RNA (lncRNA) XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells. EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h, and PCO microenvironment was simulated. The expressions levels of lncRNA XIST, miR-124, and Slug were measured by real-time polymerase chain reaction (RT-PCR) and western blot. The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by… More >