BENSHUAI YOU¹, HUI QIAN^1,2,*

BIOCELL, Vol.46, No.6, pp. 1459-1463, 2022, DOI:10.32604/biocell.2022.018378

Abstract Exosomes, especially from mesenchymal stem cells, have attracted extensive attention in regeneration medicine. Mesenchymal stem cells derived exosomes (MSCs-exosomes) have shown anti-inflammatory, anti-oxidant, anti-apoptosis and tissue regeneration effects in a variety of tissue injury repair models. MSCs-exosomes hold many excellent properties such as low immunogenicity, biocompatibility, and targeting capability. With the in-depth study on the generation and function of exosomes, MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine. However, there are still many obstacles to overcome in terms of exosomes isolation, clinical trials and safety evaluation. In this article, what we should focus on… More >

YINGHUI WU^#, HONG ZHANG^#, QIANG WANG, SUOYUAN LI, JUN SHEN^*

BIOCELL, Vol.46, No.6, pp. 1363-1364, 2022, DOI:10.32604/biocell.2022.018360

Abstract Transcription factor Brachyury, a protein containing 435 amino acids, has been widely investigated and reported in notochord differentiation and nucleus pulposus development. The crucial functions and underlying mechanisms by Brachyury are discussed in this paper, which suggests Brachyury can be developed into a potential novel target for the therapy of intervertebral disc degeneration. More >

YANQIU LI^#,*, CHENJUN HAO^#, WEIYI CHEN, QINGJU MENG

BIOCELL, Vol.46, No.6, pp. 1565-1573, 2022, DOI:10.32604/biocell.2022.018347

Abstract This work aimed to clarify the interaction between the fetus and pregnant patients with gestational diabetes mellitus (GDM), the lipid metabolomics analysis of the fetal umbilical cord blood of GDM patients and normal pregnant women were performed to screen out the specific lipid metabolites for pathogenesis of GDM. From 2019–2020, 21 patients with GDM and 22 normal pregnant women were enrolled in Hexian Memorial Hospital, Panyu District, Guangzhou. The general information such as weight, height, age, body mass index (BMI) before pregnancy were analyzed. Non-targeted metabonomic detection and analysis were performed in umbilical cord plasma using LC-MS method. The age,… More >

YANG YANG^1,*, BENSHUAI YOU², CHENGLIN ZHOU^1,*

BIOCELL, Vol.46, No.6, pp. 1557-1564, 2022, DOI:10.32604/biocell.2022.018326

Abstract The gene of Helicobacter pylori can encode three to four type IV secretory systems, of which a new gene region has been found in the H. pylori plasticity region. The coding products of this region can form a new T4SS named tfs3, but its function is unclear. This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H. pylori clinical strain SBK on GES-1 cells. We observed changes in cell morphology after VirD4 treatment. Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB. VirD4 can also inhibited proliferation, and induced… More >

NING LI^1,#, YANG CHEN^2,#, YONGJIE YANG^3,4, SHUHAN LYU¹, YUE PAN^1,5,*

BIOCELL, Vol.46, No.6, pp. 1473-1482, 2022, DOI:10.32604/biocell.2022.018278

Abstract Breast cancer is a highly aggressive cancer in females. Metastasis is a major obstacle to the efficient and successful treatment of breast cancer. Cetyltrimethylammonium bromide (CTAB) has anti-tumor effects on a variety of tumors. We showed that CTAB inhibits the metastasis of breast cancer to the lungs both in vitro and in vivo. Epithelial-mesenchymal transition (EMT) is thought to be one of the major processes mediating breast cancer metastasis. We found that CTAB suppressed EMT and regulated the levels of the classical EMT markers E-cadherin, N-cadherin, vimentin, Snail and Twist1. Moreover, as a candidate anti-tumor agent, CTAB showed primary safety… More >

XINFU FAN^1,2,#, JIE CHEN^3,#, ZONGMENG ZHANG^4,#, FANGHUI CHEN⁵, HAOSEN WANG⁶, YAFEI CAI⁶, JUN LI^7,*

BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269

Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in mice after SCI. Administration of… More >

YAO XU^1,#, BIN WU^2,#, JING YANG³, SHENG ZHANG², LONGGEN LIU⁴, SUOBAO XU^2,*, JIAKAI JIANG^2,*

BIOCELL, Vol.46, No.6, pp. 1483-1491, 2022, DOI:10.32604/biocell.2022.017745

Abstract To study the roles and association of murine double minute gene 2 (MDM2) and fructose-1,6-biphosphatase (FBP1) in human hepatocellular carcinoma (HCC), growth response of human HCC cells was assessed using proliferation and apoptosis assay. Pro-survival AKT signaling associated proteins (p-AKT, survivin and cleaved caspase 3) were assessed using western blotting. The correlation between MDM2 and FBP1 was assessed using co-immunoprecipitation combined with ubiquitination assay. Our data suggested that low expression of FBP1 was correlated with high levels of MDM2 in HCC cell lines (Huh7 and Hep3B). Overexpression of FBP1 resulted in anti-proliferation, pro-apoptosis, the up-regulation of cleaved caspase 3 while… More >

WEI LI^1,2, YUEYANG DUAN¹, XIAOTONG YAN¹, XIANGXIANG LIU¹, MEILING FAN^3,*, ZI WANG^1,2,*

BIOCELL, Vol.46, No.6, pp. 1417-1423, 2022, DOI:10.32604/biocell.2022.017609

Abstract Ginsenoside Rb3 (G-Rb3) is one of the primary active compounds isolated from Panax ginseng Meyer, which belongs to protopanaxadiol ginsenosides (PPD). Based on the structure-activity relationship (SAR) of ginsenosides, the pentose structure of G-Rb3 limited itself to possess more pharmacological activity to a certain extent. However, pharmacokinetics show that G-Rb3 is processed through deglycosylation in the intestinal tract and converted into more active rare saponins, such as Compound K, F2, etc. A series of studies focused on neuroprotection and the cardiovascular system demonstrating its therapeutic potentials, which was achieved by diminishing oxidative stress and apoptosis. Therefore, more systematic and in-depth… More >

SUYING PU^1,#, HUI ZHENG^2,#, YUN TAO¹, JING SHAO¹, MINGNA YANG¹, SHUNJUN LI^1,*

BIOCELL, Vol.46, No.6, pp. 1545-1555, 2022, DOI:10.32604/biocell.2022.017544

Abstract Intracerebral hemorrhage (ICH) is the most serious type of stroke. High level of thrombin is found in the ICH. Ubiquitin-specific protease (USP) 31, a member of deubiquitinating enzymes family, has been found to negatively regulate the NF-κB pathway. However, the function of USP31 in ICH remains largely unknown. In the present study, the mRNA and protein expression levels of USP31 were measured by real-time PCR and western blot. Flow cytometry was used to measure cell apoptosis and the level of reactive oxygen species (ROS). In the current study, we found the mRNA level of USP31 was decreased in peripheral blood… More >

JIAN XU^1,#, HAILONG GE^1,#, CHEN CHAO¹, FENG MO¹, YU WANG¹, DENGKUI ZHANG¹, XIAOXIAO ZHENG², LI ZHENG², XUEMEI LU², WEI CHEN², QUN XU^1,*, WEIXIN YU^1,*

BIOCELL, Vol.46, No.6, pp. 1493-1503, 2022, DOI:10.32604/biocell.2022.016962

Abstract Although sorafenib has been found to prolong the survival time of patients with hepatocellular carcinoma (HCC), sorafenib resistance remains an important challenge. Increasing studies have demonstrated that long noncoding RNAs (lncRNAs) contribute to drug resistance in a wide number of cancers. Human periodontal ligament stem cell (PDLSC) osteogenesis impairment-related lncRNA (POIR) is a recently defined lncRNA for which little is known regarding its function. Our study aimed to reveal the role of POIR in the development of HCC cell sorafenib resistance. The level of POIR expression in patients and tumor cells was examined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)… More >