C. DAMBORENEA*, F. BRUSA*, A. PAOLA**

BIOCELL, Vol.30, No.3, pp. 457-468, 2006, DOI:10.32604/biocell.2006.30.457

Abstract Pomacea canaliculata is a common gastropod in freshwater habitats from Central and Northern Argentina, extending northwards into the Amazon basin. Several Platyhelminthes have been reported associated to P. canaliculata, sharing an intimate relationship with this gastropod host. The objectives of this study were to describe the symbiotic species assemblages associated to P. canaliculata in the study area, and to disclose differences among them. Samples were taken in three typical small streams and one artificial lentic lagoon, all connected with the Río de la Plata estuary. The 81.53% were infested with different symbiotic (sensu lato) species. Among the Platyhelminthes, the commensal… More >

LEONOR M.E. ITUARTE*, TERESA B. VIERA*, TEOBALDO A. SALDEÑA*, JUAN C. DE ROSAS**, MABEL FÓSCOLO**, JORGE E. IBÁÑEZ*, FERNANDO D. SARAVÍ*

BIOCELL, Vol.30, No.1, pp. 43-49, 2006, DOI:10.32604/biocell.2006.30.043

Abstract Electrophysiological events occur early after fertilization, along with changes in intracellular Ca²⁺ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved.
Oocyte diameter was 70.2 ± 2.2 µm; their resting parameters were: membrane potential 23.8 ± 0.8 mV; total membrane specific resistance 519.1 ± 94.6… More >

JUAN LIN¹, XUANWEI ZHOU², JIONG FEI², ZHIHUA LIAO¹, WANG JIN¹, XIAOFEN SUN^1,*, KEXUAN TANG^1,2,*

BIOCELL, Vol.30, No.1, pp. 15-25, 2006, DOI:10.32604/biocell.2006.30.015

Abstract A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia… More >

CLAUDIA AGUILERA MERLO, ESTELA MUÑOZ, SUSANA DOMINGUEZ, MABEL FÓSCOLO*, LUIS SCARDAPANE, JUAN CARLOS DE ROSAS*

BIOCELL, Vol.29, No.3, pp. 243-251, 2005, DOI:10.32604/biocell.2005.29.243

Abstract Seasonal changes in the reproductive activity of the adult male viscacha (Lagostomus maximus maximus) were investigated during the annual reproductive cycle. Assays of heterologous in vitro binding between compatible gametes were used to evaluate the ability of viscacha spermatozoa to achieve primary binding during its annual reproductive cycle. Sperm were collected by mincing cauda epididymis in HECM-3 medium and the sperm concentration and motility were evaluated. Cumulus-free and zona-free oocytes obtained from superovulated hamsters were inseminated in vitro with capacitated sperm suspensions, incubated at 37ºC, 5% CO2 for 3 h, and then processed for studies by scanning electronic microscopy. Statistical… More >

ZHONGHAI CHEN¹, YONGZHEN PANG¹, XIAOJUN LIU¹, XINGLONG WANG¹, ZHONGXIANG DENG¹, XIAOFEN SUN¹, KEXUAN TANG^1,2

BIOCELL, Vol.29, No.2, pp. 187-193, 2005, DOI:10.32604/biocell.2005.29.187

Abstract Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had… More >

Hernández LF^1,2, MV Rosetti¹

Phyton-International Journal of Experimental Botany, Vol.85, pp. 291-296, 2016, DOI:10.32604/phyton.2016.85.291

Abstract The involucral bracts (IB or phyllaries) of the sunflower (Helianthus annuus L.) capitulum constitute an example of photosynthesizing organs that contribute to the photosynthesis budget during the generation of crop yield. The anatomy of IB was analyzed in two domesticated primitive sunflower genotypes, Havasupai and Hopi, in the sunflower line HA89B, in the wild Helianthus annuus ssp. annuus, in the sunflower male-fertile line R013 and in the commercial hybrid DKOP3845. Stomata and trichomes were counted on the adaxial and abaxial epidermis. In all cases, the IB showed a one-layered adaxial and abaxial epidermis, secretory ducts and parenchymatic cells with abundant… More >

López-España RG¹, S Hernández-Verdugo¹, S Parra-Terraza¹, F Porras¹, Antonio Pacheco-Olvera¹, A Valdez-Ortiz², T Osuna-Enciso³, MD Muy-Rangel³

Phyton-International Journal of Experimental Botany, Vol.85, pp. 131-141, 2016, DOI:10.32604/phyton.2016.85.131

Abstract Wild pepper (Capsicum annuum L. var. glabriusculum) is a valuable genetic resource for food and agriculture. The patterns of variation of 12 morphological traits of 17 populations of wild pepper collected across a latitudinal and climate gradients in northwestern Mexico were analyzed in a uniform greenhouse experiment. The morphological traits data were axamined by univariate and multivarite analysis. The relationship between phenotypic variation and latitude of population origen was tested using linear regression analysis. We found high variation within and among populations for most of the measured traits. Populations were clearly differentiated by univariate analysis of variance and principal component… More >

Valadez Sánchez YM¹, E Olivares Sáenz¹, RE Vázquez Alvarado¹, JR Esparza-Rivera³, P Preciado-Rangel⁴, RD Valdez-Cepeda⁵, JL García-Hernandez²

Phyton-International Journal of Experimental Botany, Vol.85, pp. 21-26, 2016, DOI:10.32604/phyton.2016.85.021

Abstract The aim of this study was to evaluate the effect of three organic fertilization sources on the agronomic and biochemical qualities of four genotypes of Serrano pepper. The experiment was performed at the field in Gómez Palacio, Durango (México). The capsaicinoids (CAP´s) are compounds responsible of the pungency of the peppers, and they are valuable for health and food and cosmetic food. The organic treatments applied were: solarized bovine manure (60 Mg/ha), vermicompost (10 Mg/ha) and poultry manure (5 Mg/ ha). A non-fertilized treatment was used as control. The evaluated variables were: length, diameter and individual weight on fruits, and… More >

Müller FL^1,2, LF Cyster¹, LM Raitt¹, J Aalbers¹

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 461-465, 2015, DOI:10.32604/phyton.2015.84.461

Abstract An increase in bioavailable tin in the environment could result in bioaccumulation thereof in agricultural crops, and therefore, have adverse health consequences on humans that eat these crops. The aims of the current study were thus to assess the uptake of Sn by spinach plants, and the subsequent effects this will have on the uptake of Na, Zn, K, Ca, and Mg as well as the growth of spinach plants. Spinach plants were grown in sand culture and received tin at concentrations of 0.02, 0.2, 2 and 20 mg/L along with a nutrient solution. The uptake of tin at detectible… More >

González-Mendoza D¹, A García-López¹, C Ceceña Duran¹, O Grimaldo-Juarez¹, M Aviles-Marín¹, Y Pérez-Luna², P Álvarez-Gutiérrez²

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 345-350, 2015, DOI:10.32604/phyton.2015.84.345

Abstract We studied the changes in the number of leaves, roots and height on three cultivars of Capsicum anuumm (Jalapeño, Serrano and De árbol) after inoculation with Glomus intraradices FS18 and Glomus Zac-19. Results indicated that Glomus intraradices FS18 produced greater effects on height (6.12 and 5.63 cm) and number of leaves (10.66 and 5.84) in the cultivars “Jalapeño” and “De árbol”, respectively. On the other hand, Glomus zac-19 stimulated the number of leaves and roots in “Serrano” pepper (6.17 leaves and 15.31 roots) and “Arbol” (6.52 leaves and 26.32 roots). Our results demonstrate the capacity of Glomus intraradices FS18 and… More >