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  • Open Access

    ARTICLE

    Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

    YAO QIAN2, JINGPING WANG2, ZIWAN JI2, HAO CHEN2, YUCANG HE2, XULING LV2, ZIKAI ZHANG2, TIAN LI2, TIANYUN PAN3, LIQUN LI2, MING LIN1,*

    BIOCELL, Vol.45, No.1, pp. 189-198, 2021, DOI:10.32604/biocell.2021.011827

    Abstract The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC… More >

  • Open Access

    ARTICLE

    Effects of Barium Stress in Brassica juncea and Cakile maritima: The Indicator Role of Some Antioxidant Enzymes and Secondary Metabolites

    Houda Bouslimi1, Renata Ferreira2, Nesrine Dridi1, Pedro Brito3, Susete Martins-Dias4, Isabel Caçador5, Noomene Sleimi1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 145-158, 2021, DOI:10.32604/phyton.2020.011752

    Abstract Soil contamination by toxic trace metal elements, like barium (Ba), may stimulate various undesirable changes in the metabolic activity of plants. The plant responses are fast and with, direct or indirect, generation of reactive oxygen species (ROS). To cope with the stress imposed by the ROS production, plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS, and their by-products, into stable nontoxic molecules. To assess the Ba stress response of two Brassicaceae species (Brassica juncea, a glycophyte, and Cakile maritime, a halophyte), plants were exposure to different Ba concentrations (0, 100, 200, 300 and… More >

  • Open Access

    ARTICLE

    The Allelopathic Effects of Sunflower and Wheat Root Exudates on Sinapis arvensis and Sinapis alba

    Bengu Turkyilmaz Unal1,*, Mustafa Bayram2

    Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 413-423, 2019, DOI:10.32604/phyton.2019.08244

    Abstract In this study, we aimed to investigate the allelopathic effects of sunflower and wheat root exudates on the common weeds such as wild mustard and white mustard in our region. The root exudates which were obtained by soaking 8 weeks old sunflower and wheat seedlings (20 or 40 seedlings) in 100 mL of distilled water for 3 days were applied to the leaves of wild mustard and white mustard. In order to compare the allelopathic effect, the recommended dose (1 g.da-1 ) and twice the recommended dose (2 g.da-1 ) of Gromstor (Tribenuron-methyl), a herbicide preferred by farmers for the… More >

  • Open Access

    ARTICLE

    Selenium invoked antioxidant defense system in Azolla caroliniana plant

    Hassan AMA, EM Mostafa

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 262-269, 2016, DOI:10.32604/phyton.2016.85.262

    Abstract Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages were 78%… More >

  • Open Access

    ARTICLE

    Effect of mixed salt stress on malondialdehyde, proteins and antioxidant enzymes of Leymus chinensis in three leaf colors

    Zhou C1,2, CA Busso3, YG Yang2, Z Zhang4, ZW Wang1, YF Yang5, XG Han1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 205-213, 2017, DOI:10.32604/phyton.2017.86.205

    Abstract The mixed salt stress is common in nature. Salt stress always affects plant growth. Different plant species have different adaptive capacity to salty soils. Leymus chinensis is an herbaceous plant with different leaf colors. However, little research was conducted to explore the different tolerance mechanisms to salt stress among the three different leaf colour genotypes of Leymus chinensis (grey green, transitional color, yellow green). Pot experiments for Leymus chinensis in three leaf colors were conducted under mixed salt treatments in 2010. Malondialdehyde (MDA) and protein concentrations, and the activity of various antioxidant enzymes [i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate… More >

  • Open Access

    ARTICLE

    Physiological, Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress

    Xiaoqin Zhang, Tao Tong, Bin Tian, Yunxia Fang, Jiangjie Pan, Junjun Zheng and Dawei Xue*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 253-260, 2019, DOI:10.32604/phyton.2019.06143

    Abstract Barley (Hordeum vulgare L.) is one of the most Aluminum (Al) sensitive cereal species. In this study, the physiological, biochemical, and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms. The results showed that superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were inhibited to different degrees following Al exposure. The MDA content also significantly increased with increasing Al concentrations. SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile, and the genomic template stability (GTS) decreased with increasing Al concentration and duration. These… More >

  • Open Access

    ARTICLE

    Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194

    Qunqun Hao1,#, Bo Lyu2,#, Yuhan Tang1,#, Deya Wang1, Yuanyuan Li1, Qingliang Li1, Yuhai Wang1,* and Wenqiang Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 109-117, 2019, DOI:10.32604/phyton.2019.06734

    Abstract A barley mutant, 194, was observed to exhibit a leaf spot phenotype over the whole course of its growing period. In this study, the phenotype and antioxidant competence were studied in the lesion mimic mutant 194. Plant height was slightly higher in mutant 194 than in the wild type (WT). In addition, leaf spot per plant in mutant 194 was significantly higher than in WT. Antioxidant competence, as indicated by reactive oxygen species (ROS) accumulation, antioxidant enzyme activity, and the expression of antioxidant enzyme-encoding genes was also assessed in mutant 194. Compared to the WT, mutant 194 displayed a relatively… More >

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