B. Li¹, F. Li¹, H-X. Li¹, X-C. Xu¹, M. Szczodry¹, Z-C. Yang¹, J-S. Lin², J. H-C. Wang^{1, *}

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 225-227, 2006, DOI:10.32604/mcb.2006.003.225

Abstract This article has no abstract. More >

E. K. Moioli¹, B. Shah¹, P. A. Clark¹, M. Stroscio¹, J. J. Mao¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 153-155, 2006, DOI:10.32604/mcb.2006.003.153

Abstract This article has no abstract. More >

Jie REN¹, Qian LIANG²

BIOCELL, Vol.43, No.3, pp. 199-206, 2019, DOI: 10.32604/biocell.2019.07128

Abstract This study aimed to investigate the role of high mobility group box-1 (HMGB1) expression in oral squamous cell carcinoma; HMGB1 promoted the proliferation and invasion of oral squamous cell carcinoma via activating epithelial-mesenchymal transformation (EMT). In this study, RNA transfection was used to silence the expression of HMGB1 in oral squamous cell carcinoma cells. CCK-8, cell clone formation and trans-well assays were used to detect the proliferation and invasion of cells before and after HMGB1 silencing. qRT-PCR and Western blot were used to detect changes in EMT marker protein expression before and after transfection. HMGB1 was significantly higher in OSCC… More >

Grisel Fierros-Romero^1,*, M. Elena Mellado-Rojas², Reynaldo C. Pless³, Juan Ignacio Valiente-Banuet¹, Ashutosh Sharma¹ and Elda M. Beltrán-Peña²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 131-137, 2019, DOI:10.32604/phyton.2019.05036

Abstract Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of… More >

Xiaoning Qin¹, Liqing Yuan², Hongxun Ruan¹, Lin Lin¹

BIOCELL, Vol.43, No.2, pp. 89-98, 2019, DOI:10.32604/biocell.2019.06352

Abstract Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues, presenting correlation with the degree of… More >

Qian Xiao¹, Xia Tang¹, Yuanxiu Chen¹, Han Bao¹, Lizhi Gao^1,2, Xiaobo Gong^3,*, Ping Zhang^1,4,*

Molecular & Cellular Biomechanics, Vol.16, No.3, pp. 199-210, 2019, DOI:10.32604/mcb.2019.07107

Abstract Adrenocorticotrophic hormone (ACTH), a 39-amino acid peptide hormone, has been reported in the appreciation of the proliferation of vascular smooth muscle cells (VSMCs), however, the mechanism in molecular scale supporting the appreciation remains to be elucidated. In this study, we observed that the protein expression levels of ACTH at 24 h after exposure to 15% cyclic stretch were significantly higher than that after 5% cyclic stretch. When VSMCs were treated with 1000 nM ACTH directly, Oct-1 and lamin B1 expression were both up-regulated associating with each other, and the presence of Oct-1 was found shuttling between the cytosol and nucleus.… More >

Junhao Yang¹, Chunxiao Chen^1,*, Qingyang Zang¹, Jianfei Li¹

Molecular & Cellular Biomechanics, Vol.15, No.4, pp. 203-214, 2018, DOI:10.32604/mcb.2018.03824

Abstract Pathological slide is increasingly applied in the diagnosis of breast tumors despite the issues of large amount of data, slow viewing and high subjectivity. To overcome these problems, a micrograph recognition method based on convolutional neural network is proposed for pathological slide of breast tumor. Combined with multi-channel threshold and watershed segmentation, a sample database including single cell, adhesive cell and invalid cell was established. Then, the convolution neural network with six layers is constructed, which has ability to classify the stained breast tumor cells with accuracy of more than 90%, and evaluate the proliferation level with relative error of… More >

Kefeng Liu^{1,2, §}, Zhengyu Zhang^{1,3, §}, Ting Pei¹, Ziqing Li⁴, Jingjing Wang¹, Hong Wang¹, Suning Ping¹, Lie Deng¹, Linli Wang¹, Jintao Huang⁵, Puyi Sheng⁴, Shuying Liu¹, Chaohong Li¹

Molecular & Cellular Biomechanics, Vol.14, No.2, pp. 101-123, 2017, DOI:10.3970/mcb.2017.014.099

Abstract This study was designed to investigate the effects of mechanical (MS) and/or oxidized low-density lipoprotein on proliferation and apoptosis of RAW264.7 macrophages and the underlying mechanisms. The cultured quiescent RAW264.7 macrophages were subject to stimulation with MS and/or in the presence or absence of simvastatin and then harvested for Western blot, and immunoflourecence. Either MS or alone could cause increase in cell proliferation and apoptosis, while their combination led to an additive effect. In terms of mechanisms, MS and/or significantly increased phosphorylation levels of MAPKs (ERKs, JNKs and p38MAPK), promoted the reactive oxygen species (ROS) and up-regulated DNA methylation in… More >

Joshua A. Zimmermann^*, Melissa L. Knothe Tate^∗,†,‡

Molecular & Cellular Biomechanics, Vol.8, No.4, pp. 275-296, 2011, DOI:10.3970/mcb.2011.008.275

Abstract Shape and fate are intrinsic manifestations of form and function at the cell scale. Here we hypothesize that seeding density and protocol affect the form and function of live embryonic murine mesenchymal stem cells (MSCs) and their nuclei. First, the imperative for study of live cells was demonstrated in studies showing changes in cell nucleus shape that were attributable to fixation per se. Hence, we compared live cell and nuclear volume and shape between groups of a model MSC line (C3H10T1/2) seeded at, or proliferated from 5,000 cells/cm2 to one of three target densities to achieve targeted development contexts. Cell… More >

Shu Chien^*

Molecular & Cellular Biomechanics, Vol.5, No.1, pp. 1-8, 2008, DOI:10.3970/mcb.2008.005.001

Abstract Fluid shear stress due to blood flow can modulate functions of endothelial cells (ECs) in blood vessels by activating mechano-sensors, signaling pathways, and gene and protein expressions. Laminar shear stress with a definite forward direction causes transient activations of many genes that are atherogenic, followed by their down-regulation; laminar shear stress also up-regulates genes that inhibit EC growth. In contrast, disturbed flow patterns with little forward direction cause sustained activations of these atherogenic genes and enhancements of EC mitosis and apoptosis. In straight parts of the arterial tree, laminar shear stress with a definite forward direction has anti-atherogenic effects. At… More >