Zhou C^1,2, CA Busso³, YG Yang², Z Zhang⁴, ZW Wang¹, YF Yang⁵, XG Han¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 205-213, 2017, DOI:10.32604/phyton.2017.86.205

Abstract The mixed salt stress is common in nature. Salt stress always affects plant growth. Different plant species have different adaptive capacity to salty soils. Leymus chinensis is an herbaceous plant with different leaf colors. However, little research was conducted to explore the different tolerance mechanisms to salt stress among the three different leaf colour genotypes of Leymus chinensis (grey green, transitional color, yellow green). Pot experiments for Leymus chinensis in three leaf colors were conducted under mixed salt treatments in 2010. Malondialdehyde (MDA) and protein concentrations, and the activity of various antioxidant enzymes [i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate… More >

Hernández-Camacho S¹, E Pérez-Molphe-Balch¹, AG Alpuche-Solís², JF Morales-Domínguez¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 151-162, 2017, DOI:10.32604/phyton.2017.86.151

Abstract Dehydrins or Group 2 Late Embryogenesis Abundant (LEA) proteins play an important role in the response and adaptation to different types of abiotic stresses such as droughts, high salinity and low temperatures. Using PCR techniques, we identified three gene fragments that encoded dehydrin-like proteins in three cactispecies Opuntia ficus-indica (OpfiDHN-like), Leuchtenbergia principis (LepDHN-like) and Mammillaria bombycina (MabDHN-like). Bioinformatic sequence analysis showed an identity between 96 and 97% with the Opuntia streptacantha dehydrin 1 (OpsDHN1) gene, demonstrating that the amplified fragments corresponded to dehydrin-like gene sequences, and that the designed oligonucleotides were effective for similar gene amplification in different cacti genera.… More >

M. Rodríguez, B. Buchholz, V. D’Annuzio, M. Donato, G.E. González, M. A. Goyeneche, T. Mazo, V. Pérez, L. Wilensky, R.J. Gelpi^*

BIOCELL, Vol.40, No.1, pp. 43-46, 2016, DOI:10.32604/biocell.2016.40.043

Abstract Dystrophin is a membrane-associated protein responsible for structural stability of the sarcolemma in cardiac myocytes and is very sensitive to ischemic damage. The goal of our study was to determine if ischemic preconditioning could prevent dystrophin breakdown through inhibition of matrix metalloproteinase-2 (MMP-2) activity. Isolated rabbit hearts were subjected to global ischemia with or without reperfusion in order to evaluate if dystrophin is preserved by ischemic preconditioning through MMP-2 inhibition. Ischemic preconditioning significantly reduced the infarct size induced by 30 min of ischemia and 180 min of reperfusion. Importantly, it also diminished dystrophin proteolysis and attenuated MMP-2 activity after 30… More >

T.L. Rose*, V.M. Gomes*, M. Da Cunha**, K.V.S. Fernandes***, J. Xavier-Filho***

BIOCELL, Vol.27, No.2, pp. 173-179, 2003, DOI:10.32604/biocell.2003.27.173

Abstract Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and Fusarium oxysporum conidia. Helix pomatia lectin, wheat germ agglutinin and Ulex europaeus lectin were used to identify differences in the binding of the vicilins to the surface of cells of S. cerevisiae and F. oxysporum treated with this protein. After… More >

Jiachen Wei^1,2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 103-104, 2019, DOI:10.32604/mcb.2019.07721

Abstract Phoretic flow can be generated by different types of gradient (e.g. temperature, concentration, or charge gradient) [1-3]. Within micro-to-nano confined system, the diffusio-phoretic property for proteins differs dramatically from that obtained in bulk condition, due to concentration fluctuation that emerges at microscopic level induced by specific and nonspecific interactions between protein and co-solute [4-5]. The phoretic mobility of protein individuals and complex in solute gradients can be theoretically described by continuum model [1-2] that neglects microscopic heterogeneity and determined experimentally by microfluidics [6], but the underlying mechanism of diffusio-phoretic motion for confined protein still remains unclear.
Our approach to… More >

H. Heras, R.J. Pollero

BIOCELL, Vol.26, Suppl.S, pp. 111-118, 2002

Abstract This article has no abstract. More >

MARCELO SILVA-BRIANO¹ , SANDRA LUZ MARTÍNEZ-HERNÁNDEZ¹ , ARACELI ADABACHE-ORTÍZ¹ , JAVIER VENTURA-JUÁREZ² , EVA SALINAS³ , J. LUIS QUINTANAR⁴

BIOCELL, Vol.31, No.2, pp. 225-228, 2007, DOI:10.32604/biocell.2007.31.225

Abstract Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25. More >

M. V. Kurian¹, J. M. Mullins¹, L. R. Hamilton¹, P. M. Mehl², J. K. Keevan²

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 217-218, 2006, DOI:10.32604/mcb.2006.003.217

Abstract This article has no abstract. More >

Bengü ERGÜDEN

BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517

Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >

Mahmoud MOUSTAFA^1,2, Saad ALAMRI¹, Hoida ZAKI², Naglaa LOUTFY², Tarek TAHA³, Ali SHATI¹, Mohamed ALKAHTANI¹, Sajda SIDDIQI¹

BIOCELL, Vol.43, No.2, pp. 81-88, 2019, DOI:10.32604/biocell.2019.06231

Abstract The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds… More >