Sicong Zheng^#, Jingjing Song^#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang^*, Qinglin Liu, Yuanzhi Pan

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 525-539, 2022, DOI:10.32604/phyton.2022.017629

Abstract Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially expressed proteins in the three… More >

FRANCISCO J. BARRANTES^#,*

BIOCELL, Vol.45, No.6, pp. 1479-1484, 2021, DOI:10.32604/biocell.2021.016502

Abstract Cholesterol is a major lipid in biological membranes. It not only plays a structural role but also modulates a wide range of functional properties of neurotransmitter and hormone receptors and ion channels. The membraneembedded segments of the paradigm neurotransmitter receptor for acetylcholine (nAChR) contain linear sequences of amino acids with the capacity to recognize cholesterol. These cholesterol consensus domains have been designated as “CARC” and its mirror sequence “CRAC”. CARC preferentially occurs in the exoplasmic-facing membrane leaflet, and CRAC, in the cytoplasmic-facing hemilayer. Both motifs are highly conserved among ion-channel and neurotransmitter receptor proteins in vertebrate nervous systems, where they… More >

ALAA OMRAN ALMAGRABI¹, YASER DAANIAL KHAN², SHER AFZAL KHAN^3,*

BIOCELL, Vol.45, No.5, pp. 1287-1298, 2021, DOI:10.32604/biocell.2021.013770

Abstract Phosphoaspartate is one of the major components of eukaryotes and prokaryotic two-component signaling pathways, and it communicates the signal from the sensor of histidine kinase, through the response regulator, to the DNA alongside transcription features and initiates the transcription of correct response genes. Thus, the prediction of phosphoaspartate sites is critical, and its experimental identification can be expensive, time-consuming, and tedious. For this purpose, we propose iPhosD-PseAAC, a new computational model for predicting phosphoaspartate sites in a particular protein sequence using Chou’s 5-steps rues: (1) Benchmark dataset. (2) The feature extraction techniques such as pseudo amino acid composition (PseAAC), statistical… More >

HUI ZHANG^1,3,#, SHIRUI CHEN^1,3,#, MENGTING LIU^1,2, YAOCHI WANG^1,2, CONG XIN^1,2, JING MA^1,3, XIAODONG ZHENG^1,2, YUANDI HUANG^1,2, BO ZHANG^3,4, XUEJUN ZHANG^1,2, LIANGDAN SUN^1,2,*, SEN YANG^1,3,*

BIOCELL, Vol.45, No.5, pp. 1321-1335, 2021, DOI:10.32604/biocell.2021.016524

Abstract The skin is the largest organ of the human body, and its aging is visible to the naked eye. The aging rate of men and women is slightly different. This study compared the protein expression of skin samples on the curved forearms of 11 healthy women and 9 healthy men. Quantitative proteomics analysis found that the expression of epidermal proteins in men and women of the same age group was different. Compared with female skin, in male skin, 20 proteins were upregulated, and 7 proteins were downregulated. These data suggest that men and women have differences in the speed of… More >

Fenni Lv^1,2,#, Sen Wang^3,#, Ruiping Tian¹, Peng Wang², Kang Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1645-1660, 2021, DOI:10.32604/phyton.2021.016947

Abstract Villin (VLN) is considered to be one of the most important actin-binding proteins, participates in modulating the actin cytoskeleton dynamics, plays essential role in plant development and resisting adverse environments. However, systematic studies of the VLN gene family have not been reported in cotton (Gossypium). In this study, 14 GhVLNs were identified in G. hirsutum. These GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of AtVLNs. In addition, the 14 GhVLN genes have highly conserved gene structure and motif architecture.… More >

Xin Li, Feiya Liao, Qiqi Ma, Beibei Jiang^*, Yuanzhi Pan, Cheng Luo, Xinjie Wang, Aining Ran

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1415-1423, 2021, DOI:10.32604/phyton.2021.016143

Abstract Chrysanthemum is one of the most important ornamental flowers in the world, and temperature has a significant influence on its field production. In the present study, differentially expressed proteins were investigated in the leaves of Dendranthema grandiflorum ‘Jinba’ under high temperature stress using label-free quantitative proteomics techniques. The expressed proteins were comparatively identified and analyzed. A total of 1,463 heat-related, differentially expressed proteins were successfully identified by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS), and 1,463 heat-related, differentially expressed proteins were successfully identified by mass spectrometry after a high temperature treatment. Among these, 701 proteins were upregulated and 762 proteins were downregulated.… More >

Sara Saber¹, Uswah Khairuddin^2,*, Rubiyah Yusof², Ahmed Madani¹

CMC-Computers, Materials & Continua, Vol.68, No.3, pp. 3563-3576, 2021, DOI:10.32604/cmc.2021.017769

Abstract The identification of DNA binding proteins (DNABPs) is considered a major challenge in genome annotation because they are linked to several important applied and research applications of cellular functions e.g., in the study of the biological, biophysical, and biochemical effects of antibiotics, drugs, and steroids on DNA. This paper presents an efficient approach for DNABPs identification based on deep transfer learning, named “DTLM-DBP.” Two transfer learning methods are used in the identification process. The first is based on the pre-trained deep learning model as a feature’s extractor and classifier. Two different pre-trained Convolutional Neural Networks (CNN), AlexNet 8 and VGG… More >

LORENZO ALIBARDI¹, BERND NOECKER²

BIOCELL, Vol.45, No.4, pp. 1013-1022, 2021, DOI:10.32604/biocell.2021.013913

Abstract The differentiation of cells composing mature human hairs produces layers with different corneous characteristics that would tend to flake away one from another, as in the corneous layer of the epidermis, without anchoring junctions. It is likely that cell junctions established in the forming cells of the hair bulb are not completely degraded like in the corneous layer of the epidermis but instead remain in the hair shaft to bind mature cuticle, cortex, and medulla cells into a compact hair shaft. During cell differentiation in hairs, cell junctions seem to disappear, and little is known about the fate of junctional… More >

YAO QIAN², JINGPING WANG², ZIWAN JI², HAO CHEN², YUCANG HE², XULING LV², ZIKAI ZHANG², TIAN LI², TIANYUN PAN³, LIQUN LI², MING LIN^1,*

BIOCELL, Vol.45, No.1, pp. 189-198, 2021, DOI:10.32604/biocell.2021.011827

Abstract The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H₂O₂ (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC… More >

EMILIA MANOLE^1,2,#, LAURA DUMITRESCU^2,3,#, CRISTINA NICULIȚE^1,3, BOGDAN OVIDIU POPESCU^1,2,3, LAURA CRISTINA CEAFALAN^1,3,*

BIOCELL, Vol.45, No.1, pp. 1-16, 2021, DOI:10.32604/biocell.2021.013452

Abstract An increasing number of studies provide evidence for the existence of a microbiota-gut-brain axis and its potential involvement in the development of sporadic Parkinson’s disease and other neurodegenerative conditions. The neuropathologic hallmark of Parkinson’s disease is the presence of brain intraneuronal aggregates of misfolded alpha-synuclein, known as Lewy bodies. Some gut microbiota products may trigger alpha-synuclein conformational changes in the neurons of the enteric nervous system, which can then spread to the brain in a prion-like fashion through the vagus nerve. Others may interfere with neuroinflammatory pathways and susceptibility to neurodegeneration. In this review, we assess the potential role of… More >