Iván De-la-Cruz-Chacón, Christian Anabí Riley-Saldaña, Marisol Castro-Moreno, Claudia Azucena Durán-Ruiz, Alma Rosa González-Esquinca^*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3509-3526, 2025, DOI:10.32604/phyton.2025.072586 - 01 December 2025

Abstract The seedling phase represents an initial and critical stage for the establishment of individuals in the ecosystem. During this stage, specialized metabolites contribute to survival; however, studies analyzing the presence of these molecules and the reasons for their production and accumulation are still scarce. Annonaceae is a botanical family recognized for the chemodiversity of its secondary metabolites; nearly 1000 alkaloids have been reported in approximately 150 adult specimens. The aim of this study was to determine whether alkaloid biosynthesis in Annonaceae is expressed from early stages. For this purpose, Annona macroprophyllata, Annona muricata, Annona purpurea, and Annona reticulata seedlings,… More >

Tetiana O. Yastreb^1,2, Mykola V. Shevchenko², Liubov N. Kobyzeva^1,3, Alexander I. Oboznyi¹, Yuriy E. Kolupaev^1,2,4,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3125-3143, 2025, DOI:10.32604/phyton.2025.071822 - 29 October 2025

Abstract β-Aminobutyric acid (BABA) is a physiologically active plant compound that has not been extensively studied. It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species. However, its effects on cereal grains with low germination rates have not yet been studied. This study investigated the effects of BABA on the germination of aged triticale seeds, the metabolite content of seedlings, and the state of their antioxidant systems. The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to… More > Graphic Abstract

Amine Assouguem^1,*, Saoussan Annemer^2,3, Mohammed Kara⁴, Abderrahim Lazraq⁵

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1631-1668, 2025, DOI:10.32604/phyton.2025.065750 - 27 June 2025

Abstract Unlike primary metabolites, secondary metabolites serve critical ecological functions, including plant protection, stress tolerance, and symbiosis. This review focuses on extracting, separating, and identifying the major classes of secondary metabolites, including alkaloids, terpenoids, phenolics, glycosides, saponins, and coumarins. It describes optimized methods regarding plant selection, extraction by solvents, and purification of the metabolites, highlighting the latest advancements in chromatographic and spectroscopic techniques. The review also describes some of the most important problems, such as the instability of the compounds or diversity of the structures, and discusses emerging technologies that solve these issues. Moreover, it examines More >

Chen Chen^#,*, Chunli Li^#, Tengfei Li, Qianhong Li, Luyao Li, Fengqin Liu

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 793-807, 2025, DOI:10.32604/phyton.2025.063549 - 31 March 2025

Abstract Lycium ruthenicum Murray, a plant widely cultivated in northwestern China, is integral to traditional Chinese medicine, with applications in treating menstrual disorders, cardiovascular diseases, and menopausal symptoms. Despite its recognized medicinal value and use as a functional food, comprehensive knowledge of its metabolites and their pharmacological effects remains limited. This study presents an innovative approach using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF/MS) to conduct a detailed analysis of both wild and cultivated L. ruthenicum samples. A total of 62 peaks were detected in the total ion current profile, with 59 metabolites identified based… More >

Fayez Althobaiti¹, Hadeer Darwish^1,*, Raghad Alruqayb¹, Saqer S. Alotaibi¹, Fahad E. Alharthi², Ibrahim Jafri¹, Sarah Awwadh Altalhi¹, Ghadi Alsharif^3,4, Hussam Awwadh E Althagafi⁵, Hesham Noureldeen⁶, Sarah Alharthi⁷, Jawaher Albaqami², Najla Amin T. Al Kashgry², Ahmed Noureldeen²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 181-198, 2025, DOI:10.32604/phyton.2025.059387 - 24 January 2025

Abstract The interest in using the Datura stramonium plant is due to its natural products, which are used in many pharmaceutical industries. The objective of the current study was to assess the therapeutic and cytotoxic effects of the D. stramonium plant on two types of human cancer cell models (MCF7 and HT29) in vitro. A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves. The recovered crude, after the solvent had evaporated, was then dispersed at varied concentrations of extract 100, 50, 20, and 0.0 µg/mL and tested to see how the cells responded. Also,… More >

Adil Mihoub^1,*, Mohammed Mesnoua¹, Nabil Touzout², Reguia Zeguerrou¹, Nourelislm Siabdallah¹, Chawqi Benchikh¹, Saliha Benaoune¹, Aftab Jamal³, Domenico Ronga⁴, Jakub Černý^5,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2993-3011, 2024, DOI:10.32604/phyton.2024.057536 - 30 November 2024

Abstract Globally, salinity is a brutal environmental constraint that poses a major threat to agriculture worldwide, causing nutrient imbalances and oxidative stress, leading to reduced crop yields and quality. Date palm waste from the agro-industry is a major environmental problem, but its conversion to biochar for soil amendment could help alleviate the effects of salinity stress. Pepper is a commonly grown horticultural crop that is sensitive to salinity. That’s why the current experiment was conducted with the novel idea of exploring the potential use of biochar-based fertilizer derived from date palm waste as a mitigation strategy… More >

Wissal Benaffari^1,2,3, Fatima-Ezzahra Soussani^1,2,4, Abderrahim Boutasknit^1,2,5, Salma Toubali^1,2,3, Abir Ben Hassine³, Hala Ben Ahmed³, Rachid Lahlali^7,*, Abdelilah Meddich^1,2,6,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2285-2302, 2024, DOI:10.32604/phyton.2024.055052 - 30 September 2024

Abstract Quinoa (Chenopodium quinoa) has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds, and its capacity to persist in water-stressed environments. Our study aimed to assess the effects of native arbuscular mycorrhizal fungi (AMF) and local organic amendments on the metabolic responses and antioxidant activity of quinoa seeds under water-stressed conditions. To this end, quinoa plants were grown in soils inoculated with an indigenous mycorrhizal consortium AMF and amended with two types of compost from horse manure (HM) and green waste (GW) under two water… More >

Mingzhao Han¹, Susilawati Kasim^1,*, Zhongming Yang², Xi Deng², Noor Baity Saidi³, Md Kamal Uddin¹, Effyanti Mohd Shuib¹

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2149-2166, 2024, DOI:10.32604/phyton.2024.054009 - 30 September 2024

Abstract It is imperative to enhance crop yield to meet the demands of a burgeoning global population while simultaneously safeguarding the environment from adverse impacts, which is one of the dominant challenges confronting humanity in this phase of global climate change. To overcome this problem and reduce dependency on chemical fertilizer, scientists now view the implementation of biostimulant strategies as a cost-effective and environmentally friendly approach to achieving sustainable agriculture. Plant extracts are rich in bioactive phytocompounds, which can enhance plant resistance to disease, pest, and abiotic stresses (e.g., drought, salinity, and extreme temperature), and promote… More >

Ayda Khadhri¹, Mohamed Mendili¹, Marwa Bannour-Scharinger¹, Eric Masson², Antonio Pizzi^2,*

Journal of Renewable Materials, Vol.12, No.4, pp. 827-842, 2024, DOI:10.32604/jrm.2024.046950 - 12 June 2024

Abstract This study is the first to evaluate the phytochemical content and biological properties of Tunisian T. platyphyllos Scop. A total of 23 compounds of essential oils were identified by gas chromatography-mass spectrometry (GC-MS) analysis of bracts and fruit extracts. The results show that oxygenated monoterpenes were the dominant class of essential oils. The phenolic composition was investigated by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The analysis showed that the chemical profiles of the ethanolic extracts of bracts and fruits are substantially similar. The highest polyphenol content was found in the ethanolic extracts of the fruits (7.65… More > Graphic Abstract

JHILAM PRAMANIK^1,#, AKASH KUMAR², SARVESH RUSTAGI^3,#, MEHAK KATYAL⁴, SHEETAL THAKUR⁵, JUTISHNA BORA⁶, SUMIRA MALIK^6,7,*, ANCHAL TREHAN⁸, NAYAN TALUKDAR⁹, PETR SLAMA^10,*

BIOCELL, Vol.48, No.4, pp. 541-557, 2024, DOI:10.32604/biocell.2023.029031 - 09 April 2024

Abstract Many phytochemicals and their derived metabolites produced by plants are extensively employed in commercial goods, pharmaceutical products as well as in the environmental and medical fields. However, these secondary metabolites obtained from plants are in low amounts, and it is difficult to synthesize them at the industrial level. Despite these challenges, they may be utilized for a variety of medicinal products that are either available in the market or are being researched and tested. Secondary metabolites are complex compounds that exhibit chirality. Further, under controlled conditions with elicitors, desired secondary metabolites may be produced from More > Graphic Abstract