MINGMING FANG^1,#, NING GE^2,#, JIANFANG LIU^3,*, YAYUN CUI^2,*

BIOCELL, Vol.46, No.3, pp. 711-720, 2022, DOI:10.32604/biocell.2022.016714 - 18 November 2021

Abstract The resistance of cancer cells to the anti-cancer drugs is the most important reason that affecting the efficacy of the non-small cell lung cancer (NSCLC) chemotherapy; thus, to explore the underlying mechanism of drug resistance of NSCLC medications is urgently needed for improving the therapeutic efficacy of current anti-NSCLC chemotherapies. The aim of the present study is to explore the roles of exosomes in the chemosensitivity of A549 cells and the related mechanism. A549 cells and cisplatin resistant cell line A549/DDP derived exosomes were isolated, and the expressions of CXCR4 were compared. Then, after cisplatin… More >

R. Joshua Samuel Raj^1,*, J. Prince Antony Joel², Salem Alelyani³, Mohammed Saleh Alsaqer³, C. Anand Deva Durai⁴

CMC-Computers, Materials & Continua, Vol.71, No.1, pp. 1171-1188, 2022, DOI:10.32604/cmc.2022.021667 - 03 November 2021

Abstract Human Adaptive Mechatronics (HAM) includes human and computer system in a closed loop. Elderly person with disabilities, normally carry out their daily routines with some assistance to move their limbs. With the short fall of human care takers, mechatronics devices are used with the likes of exoskeleton and exosuits to assist them. The rehabilitation and occupational therapy equipments utilize the electromyography (EMG) signals to measure the muscle activity potential. This paper focuses on optimizing the HAM model in prediction of intended motion of upper limb with high accuracy and to increase the response time of… More >

TAILIN WU^1,#, XIANG ZHOU^2,#, CANHUA YE¹, WENCAN LU¹, HAITAO LIN¹, YANZHE WEI¹, ZEKAI KE¹, ZHENGJI HUANG¹, JIANZHOU LUO¹, HUIREN TAO¹, CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.2, pp. 495-503, 2022, DOI:10.32604/biocell.2022.015214 - 20 October 2021

Abstract Differentiated macrophages have been proven to participate in the development of mesenchymal stem cells in different tissues. However, the regulatory processes remain obscure. Exosomes, which are key secretions of macrophages, have attracted increasing attention. Therefore, macrophage-derived exosomes may modulate the development of Bone marrow mesenchymal stem cells (BMMSCs). Different culture conditions were used to induce M1 polarization in THP1 cells. Subsequently, exosomes derived from unpolarized (M0) and polarized (M1) macrophages were isolated, BMMSCs were cultured with normal complete medium or inductive medium supplemented with M0 or M1 derived exosomes, and the osteogenic capacity of the… More >

HAIZHENG LIU¹, SHAOFEI CHANG^2,*

BIOCELL, Vol.46, No.2, pp. 453-462, 2022, DOI:10.32604/biocell.2021.015340 - 20 October 2021

Abstract Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a phospholipid acyltransferase that promotes phospholipid synthesis and plasma membrane reconstruction. Exosomes play an important role in tumor metastasis. The release and uptake of exosomes are key steps of their functions and depend on plasma membrane fusion and plasma membrane receptors, respectively. The purpose of this study was to explore whether LPCAT1-induced plasma membrane remodeling would change the secretion and uptake behavior of exosomes in tumor cells. We first confirmed the abnormally high expression of LPCAT1 in colorectal cancer cells by quantitative real-time PCR (qPCR) and Western blot analysis. Then,… More >

ZHIHENG LIU^1,2, ZHEN SUN³, ZHONGYUAN WAN⁴, HAIQIANG WANG^2,*

BIOCELL, Vol.46, No.2, pp. 511-517, 2022, DOI:10.32604/biocell.2021.016194 - 20 October 2021

Abstract We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration (IDD). However, it remains elusive regarding miR-155 expression in peripheral blood mononuclear cells (PBMCs) and local extracellular space in IDD. The study aimed for investigating the miR-155 expression of PBMCs, extracellular miRNAs (ex-miRNAs) of human nucleus pulposus (NP) tissues, and morphological changes of cell death in the IDD process. Here, we harvested peripheral blood and NP samples from scoliosis and IDD patients as control and degenerative groups, respectively. Then standard Ficoll density-gradient centrifugation was used to isolate PBMCs. The two subpopulations of PBMCs… More >

Talha Ahmed Khan, Khizar Abbas, Afaq Muhammad, Wang-Cheol Song^*

CMC-Computers, Materials & Continua, Vol.70, No.3, pp. 4323-4340, 2022, DOI:10.32604/cmc.2022.017118 - 11 October 2021

Abstract The scope of the 5G network is not only limited to the enhancements in the form of the quality of service (QoS), but it also includes a wide range of services with various requirements. Besides this, many approaches and platforms are under the umbrella of 5G to achieve the goals of end-to-end service provisioning. However, the management of multiple services over heterogeneous platforms is a complex task. Each platform and service have various requirements to be handled by domain experts. Still, if the next-generation network management is dependent on manual updates, it will become impossible… More >

Muhatasim Intisar¹, Mohammad Monirujjaman Khan^1,*, Mehedi Masud², Mohammad Shorfuzzaman²

Intelligent Automation & Soft Computing, Vol.31, No.1, pp. 101-115, 2022, DOI:10.32604/iasc.2022.019083 - 03 September 2021

Abstract Paralysis is detrimental to people in catastrophic ways: losing income opportunities, becoming a burden to their friends and family, further physical deterioration, and the combination of these occurrences can often lead to depression. In third world countries, suffering from paralysis can be extra deleterious, where a large proportion of the population is engaged in some form of physical labor. The number of people exposed to paralysis risk factors is also increasing, with more and more people having hypertension, smoking, and other abnormalities. Besides, low workplace safety precautions may lead to an increased risk of spinal… More >

JIAFU FENG^1,2,#,*, BEI XU^1,2,#, CHUNMEI DAI^1,2,#, YAODONG WANG^1,4, GANG XIE^1,5, WENYU YANG^1,2, BIN ZHANG^1,2, XIAOHAN LI⁶, JUN WANG³

Oncology Research, Vol.29, No.5, pp. 331-349, 2021, DOI:10.32604/or.2022.03554 - 10 October 2022

Abstract Due to its difficulty in early diagnosis and lack of sensitivity to chemotherapy and radiotherapy, renal cell carcinoma (RCC) remains to be a frequent cause of cancer-related death. Here, we probed into new targets for its early diagnosis and treatment for RCC. microRNA (miRNA) data of M2-EVs and RCC were searched on the Gene Expression Omnibus database, followed by the prediction of the potential downstream target. Expression of target genes was measured via RT-qPCR and Western blot, respectively. M2 macrophage was obtained via flow cytometry with M2-EVs extracted. The binding ability of miR-342-3p to NEDD4L… More >

PENGFEI ZHANG¹, SHAOPENG WANG^1,2,*

BIOCELL, Vol.45, No.6, pp. 1449-1451, 2021, DOI:10.32604/biocell.2021.017607 - 01 September 2021

Abstract The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity. More >

SUSHMITHA SRIRAMULU¹, SARUBALA MALAYAPERUMAL¹, SUMAN K. NANDY², ANTARA BANERJEE¹, MUSTHAFA MOHAMED ESSA^3,4, SARAVANABABU CHIDAMBARAM⁵, M. WALID QORONFLEH^6,7, SURAJIT PATHAK^1,*

BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756 - 03 March 2021

Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to… More >