Sumeyra Ucar¹, Muhammed Aldaif ², Esra Yaprak¹, Esma Yigider ², Murat Aydin^2,*, Emre Ilhan¹, Abdulkadir Ciltas², Ertan Yildirim³

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2461-2477, 2025, DOI:10.32604/phyton.2025.068383 - 29 August 2025

Abstract Wheat is a crucial crop for global food security, and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs. A practical approach for in vitro regeneration of the Kırik bread wheat cultivar via somatic embryogenesis was investigated using endosperm-supported mature embryos. Callus cultures were initiated from mature embryos supported by endosperm, cultured on phytagel-based Murashige and Skoog (MS) basal medium containing dicamba (12 mg/L) and indole-3-acetic acid (IAA) (0.5 mg/L) under dark conditions. This research was designed to examine the impact of putrescine (Put) (0.0 and 1.0 mM) on… More >

Xinghuan Li¹, Binxi Hao¹, Shimin Cheng¹, Ju Zhang¹, Yuan Song^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2499-2513, 2025, DOI:10.32604/phyton.2025.067104 - 29 August 2025

Abstract Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China, known as the mixed broadleaf-Korean pine forest. Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P. koraiensis, it remains unclear whether these metabolic pathways function differentially across tissues. P. koraiensis seeds that had undergone different durations of moist chilling in their natural environment, yielding seeds with relatively deeper primary physiological dormancy (DDS) and seeds with released primary physiological dormancy (RDS). A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon… More >

Shijie Lin¹, Zimo Wang¹, Hongbo Zhu², Conghui Wang³, Hongfeng Wang², Dawei Zhang¹, Tianbing Gou¹, Guangdao Bao¹, Ye Luo¹, Huaijiang He¹, Zhonghui Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 277-289, 2024, DOI:10.32604/phyton.2024.046989 - 27 February 2024

Abstract Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture medium for… More >

Laura Isabel Arias-Rodríguez¹, Martha Alicia Rodríguez-Mendiola^2,*, Carlos Arias-Castro^2,*, Federico Antonio Gutiérrez Miceli¹, Diana Reséndez Pérez³, María Celina Luján Hidalgo¹, Juan José Villalobos Maldonado¹, Norma Alejandra Mancilla Margalli²

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2807-2823, 2023, DOI:10.32604/phyton.2023.030396 - 15 September 2023

Abstract In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration. In our study, we specifically investigated the extract of Petroselinum crispum, a plant known for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly… More >

Xuan Sun^1,2, Xue Wang¹, Bijaya Sharma Subedi³, Yin Jiang^1,2, Di Wang^1,2, Rongxin Gou^1,2, Guojun Zhang², Wenting Xu^4,*, Zunzheng Wei^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2413-2428, 2023, DOI:10.32604/phyton.2023.029667 - 25 June 2023

Abstract The calla lily (Zantedeschia spreng.) is a bulbous flower native to the tropical regions of Africa. Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration. Despite such advantages, for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, there are challenges in terms of hybrid production due to the ‘plastome-genome incompatibility’ therebetween. Tissue culture is a fundamental biotechnological tool used in gene editing research, with a focus on disease resistance and flower color in calla lily breeding programs. The present review provides a brief… More >

YANG CAO^1,#, YINING LIU^1,#, YANG YU¹, XIAOFEI GUO¹, XIUXIU WANG¹, WENYA MA¹, HANJING LI², ZHONGYU REN², XINLU GAO², SIJIA LI², HAOYU JI², HONGYANG CHEN², HONG YAN², YANAN TIAN², XIN WANG², BENZHI CAI^1,2,*

BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080 - 19 May 2023

Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression… More >

JESSICA CRISTINA MARÍN-LLERA^*, CARLOS AMAURY JIMÉNEZ-CÁRDENAS, JESÚS CHIMAL-MONROY^*

BIOCELL, Vol.47, No.3, pp. 465-471, 2023, DOI:10.32604/biocell.2023.024625 - 03 January 2023

Abstract The molecular cascade underlying tendon formation starts when progenitor cells begin to express the Scleraxis (Scx) gene. Scx knockout mice develop some but not all tendons, suggesting that additional factors are necessary for tendon commitment, maintenance, and differentiation. Other transcription factors, such as Mohawk (Mkx) or early growth response (Egr), maintain Scx expression and extracellular matrix formation during fibrillogenesis. The inhibition of wingless and int-related protein signaling is necessary and sufficient to induce the expression of Scx. Once the commitment of tenogenic lineage occurs, transforming growth factor-beta (TGFβ) induces the Scx gene expression, becoming involved in the maintenance of tendon More >

TINGTING HUANG^1,#, SHAN DENG^2,#, JIANGYUAN SHENG¹, DI ZHANG^1,*

BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314 - 07 July 2022

Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK₃ type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE… More >

Xiaotong Wei^1,#, Xuhong Fan^2,#, Xuan Lu¹, Liyuan Han¹, Honglin Zhang³, Siyan Liu³, Jing Qu³, Shuyan Guan^3,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062 - 13 May 2022

Abstract

The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A₂B₃C₁D₃₍₂₎, namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL^-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL^-1, and the concentration of silver nitrate (AgNO₃) was 10 mg·mL^-1 (or 5 mg·mL).

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XIAOXIA WANG^1,#, ZHONGYUAN YAO^1,2,#, DI LIU¹, CHUNHONG YU^3,*, HUI LI^1,3,*

BIOCELL, Vol.46, No.8, pp. 1803-1806, 2022, DOI:10.32604/biocell.2022.019731 - 22 April 2022

Abstract Each stage of embryonic development, including normal gamete maturation, fertilization, zygotic genome activation, and cleavage, is crucial for human reproduction. Early embryo arrest is a common phenomenon. It is estimated that about 40%–70% of human embryos are arrested at early developmental stages. However, the exact mechanism remains largely uncertain. Embryos can be investigated in vitro by way of the development of in vitro fertilization/intracytoplasmic sperm injection. In addition to iatrogenic factors related to abnormal oocyte/embryo development, multiple gene mutations have been found to be involved in such phenotypes. Based on the knowledge of known etiological factors, several More >