WANG JIANMIN, SONG RUIHUA, CHEN LEI, YIN LIANGJUN, CHEN BO, SUN JING, GOU YUANBING, ZHAO LING, CHEN LIN

BIOCELL, Vol.30, No.2, pp. 269-278, 2006, DOI:10.32604/biocell.2006.30.269

Abstract Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot… More >

G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI

BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209

Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed… More >

M.T. TERUEL^1,2, R.C. CATALANO², J.A. CABODEVILA², S.S. CALLEJAS²

BIOCELL, Vol.29, No.2, pp. 183-186, 2005, DOI:10.32604/biocell.2005.29.183

Abstract Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h… More >

CECILIA I. IBAÑEZ DE BARRETT, JUAN PABLO BOZZINI, MARTA MARIANO DE BOZZINI*

BIOCELL, Vol.28, No.3, pp. 259-269, 2004, DOI:10.32604/biocell.2004.28.259

Abstract The post-embryonic development of the female gonads in Triatoma infestans (Hemiptera, Heteroptera), insects of importance in health affairs as harbors and vectors of different tripanosomatidea flagellates, is presented in a complete follow-up since insect hatches from the egg up to the last molt in the fifth instar stage. The detailed description of the morphological changes which occur in each instar as well as careful measurements evaluating its size increase have been analyzed by stereomicroscopy, phase contrast, dark field, and oblique illumination, in order to optimize the observations as well the photographic register of gonad morphology and structure.
The analysis… More >

MIRIAM TERUEL^1,2, JAQUELINE D’ERCOLE¹ AND RODOLFO CATALANO²

BIOCELL, Vol.35, No.1, pp. 29-34, 2011, DOI:10.32604/biocell.2011.35.029

Abstract Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Females were superovulated by intraperitoneal injection of 10 IU of eCG and 10 IU of hCG (48h later) and were paired with males of proven fertility. Albendazole sulphoxide (200 mg/kg) was orally administered by gavages at day 1, 2 or 3 of pregnancy; the control group received only the vehicle (carboxymethylcellulose). Females… More >

Guan Z-J^1,3,4*, S-B Lu^2*,Y-L Huo⁵, B Guo³, Z-P Guan⁶, Y-H Wei³

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

Perissé P¹ y GM Tourn²

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 184-189, 2015, DOI:10.32604/phyton.2015.84.184

Abstract The morphological characteristics of the seeds of Vicia dasycarpa Ten. cv. Tolse FCA were studied in relation to the area of imbibition water entry and were considered the presence of areoles. Seeds were analyzed using a stereo, optical and scanning electron microscope (SEM). The determination of the initial water entry area was carried out by immersing the seeds in a solution of tetrazolium (1%). This study showed that this species has seeds with a halo framing the hilum, an inconspicuous dry aril and a deltoid micropyle. The seedcoat pattern is papillose. The tracheid bar is surrounded by a ring of… More >

Guan Z-J^1,2,*, B Guo^1,*, Y-L Huo³, J-K Dai⁴, Y-H Wei¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051

Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >

Menéndez-Yuffá¹ A, L Ríos-Bolívar²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

Peng M^1,2, FL Huang^1*, FJ Meng², BZ Hu^3*, XF Chen¹, R Luo⁴, N Li¹, RF Wang¹, Y Zhao¹, QW Zou¹, CT Wu¹, JL Dai⁵

Phyton-International Journal of Experimental Botany, Vol.86, pp. 296-305, 2017, DOI:10.32604/phyton.2017.86.296

Abstract Chinese herbaceous peony (Paeonia lactiflora Pall.) is a popular ornamental plant. However, little is known about its reproductive biology by histological analyses. Here, we used the paraffin wax method to study the reproductive biology of the Chinese herbaceous peony (Paeonia lactiflora ‘Dafugui’). The results showed that the flower bud of Chinese herbaceous peony was a mixed bud. The course of flower bud differentiation included some developmental morphology periods, such as bract, calyx, petal, stamen, and pistil. The anther wall development was related to a dicotyledonous type. Pollen was mainly 2-celled, only few of them were 3-celled. Moreover, the mature pollen… More >