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  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Analysis of Calmodulin-Like Proteins in Tobacco

    Mengjie Xu, Anbin Wang, Tonghong Zuo, Hecui Zhang, Zhihao Hu, Liquan Zhu*

    Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 157-179, 2025, DOI:10.32604/phyton.2025.060566 - 24 January 2025

    Abstract Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally More >

  • Open Access

    ARTICLE

    XGBoost Based Multiclass NLOS Channels Identification in UWB Indoor Positioning System

    Ammar Fahem Majeed1,2,*, Rashidah Arsat1, Muhammad Ariff Baharudin1, Nurul Mu’azzah Abdul Latiff1, Abbas Albaidhani3

    Computer Systems Science and Engineering, Vol.49, pp. 159-183, 2025, DOI:10.32604/csse.2024.058741 - 03 January 2025

    Abstract Accurate non-line of sight (NLOS) identification technique in ultra-wideband (UWB) location-based services is critical for applications like drone communication and autonomous navigation. However, current methods using binary classification (LOS/NLOS) oversimplify real-world complexities, with limited generalisation and adaptability to varying indoor environments, thereby reducing the accuracy of positioning. This study proposes an extreme gradient boosting (XGBoost) model to identify multi-class NLOS conditions. We optimise the model using grid search and genetic algorithms. Initially, the grid search approach is used to identify the most favourable values for integer hyperparameters. In order to achieve an optimised model configuration,… More >

  • Open Access

    ARTICLE

    Deep learning identification of novel autophagic protein-protein interactions and experimental validation of Beclin 2-Ubiquilin 1 axis in triple-negative breast cancer

    XIANG LI1,#, WENKE JIN2,#, LIFENG WU2, HUAN WANG1, XIN XIE1, WEI HUANG1,*, BO LIU2,*

    Oncology Research, Vol.33, No.1, pp. 67-81, 2025, DOI:10.32604/or.2024.055921 - 20 December 2024

    Abstract Background: Triple-negative breast cancer (TNBC), characterized by its lack of traditional hormone receptors and HER2, presents a significant challenge in oncology due to its poor response to conventional therapies. Autophagy is an important process for maintaining cellular homeostasis, and there are currently autophagy biomarkers that play an effective role in the clinical treatment of tumors. In contrast to targeting protein activity, intervention with protein-protein interaction (PPI) can avoid unrelated crosstalk and regulate the autophagy process with minimal interference pathways. Methods: Here, we employed Naive Bayes, Decision Tree, and k-Nearest Neighbors to elucidate the complex PPI… More >

  • Open Access

    REVIEW

    Structural Modal Parameter Recognition and Related Damage Identification Methods under Environmental Excitations: A Review

    Chao Zhang1, Shang-Xi Lai1, Hua-Ping Wang1,2,*

    Structural Durability & Health Monitoring, Vol.19, No.1, pp. 25-54, 2025, DOI:10.32604/sdhm.2024.053662 - 15 November 2024

    Abstract Modal parameters can accurately characterize the structural dynamic properties and assess the physical state of the structure. Therefore, it is particularly significant to identify the structural modal parameters according to the monitoring data information in the structural health monitoring (SHM) system, so as to provide a scientific basis for structural damage identification and dynamic model modification. In view of this, this paper reviews methods for identifying structural modal parameters under environmental excitation and briefly describes how to identify structural damages based on the derived modal parameters. The paper primarily introduces data-driven modal parameter recognition methods… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Pattern Analysis of LONELY GUY Gene Family in Walnut (Juglans regia)

    Yuan Wang1, Tianle Zhang1, Xinfeng Zeng1, Jiale Liu1, Siyu Li1, Siyu Yang1, Shengnan Zhao1, Abdullah Shah2, Muhammad Saif Ullah2, Guohui Qi1,*, Peng Jia1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3331-3346, 2024, DOI:10.32604/phyton.2024.059402 - 31 December 2024

    Abstract LONELY GUY (LOG) is a pivotal cytokinin-activating enzyme that plays an important role in plant growth, development, and stress responses. Walnut (Juglans regia), an important woody oilseed species, has not yet undergone systematic identification of its LOG gene family. In this study, we identified 17 JrLOG genes in the walnut genome, which are unevenly distributed across 11 chromosomes. JrLOG gene expansion was primarily driven by gene duplication, along with purifying selection. Members of the JrLOG family were categorized into five groups, each exhibiting analogous gene structures, featured motifs, and conserved domains. Transcriptome and quantitative real-time PCR analyses More >

  • Open Access

    ARTICLE

    Construction of a Yeast Hybrid Library and Identification of Proteins Regulating CaABI3/VP1-1 Expression in Capsicum annuum var. conoides

    Huiru Deng#, Huan Tian#, Liuyan Yang, Siyang Ou, Hui Wang, Guangdong Geng*, Suqin Zhang*

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3273-3291, 2024, DOI:10.32604/phyton.2024.058638 - 31 December 2024

    Abstract Hot pepper (Capsicum annuum var. conoides) is a significant vegetable that is widely cultivated around the world. Currently, global climate change has caused frequent severe weather events, and waterlogging stress harms the pepper industry by affecting the planting period, growth conditions, and disease susceptibility. The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance. In order to explore the upstream regulatory mechanism of CaABI3/VP1-1, a high-quality standardized yeast hybrid library was successfully constructed for yeast one-, two-, and three-hybrid screening using pepper ‘ZHC2’ as the experimental material, with a library recombinant efficiency of up to 100%. The length of inserted… More >

  • Open Access

    ARTICLE

    A DDoS Identification Method for Unbalanced Data CVWGG

    Haizhen Wang1,2,*, Na Jia1,2, Yang He1, Pan Tan1,2

    CMC-Computers, Materials & Continua, Vol.81, No.3, pp. 3825-3851, 2024, DOI:10.32604/cmc.2024.055497 - 19 December 2024

    Abstract As the popularity and dependence on the Internet increase, DDoS (distributed denial of service) attacks seriously threaten network security. By accurately distinguishing between different types of DDoS attacks, targeted defense strategies can be formulated, significantly improving network protection efficiency. DDoS attacks usually manifest as an abnormal increase in network traffic, and their diverse types of attacks, along with a severe data imbalance, make it difficult for traditional classification methods to effectively identify a small number of attack types. To solve this problem, this paper proposes a DDoS recognition method CVWGG (Conditional Variational Autoencoder-Wasserstein Generative Adversarial… More >

  • Open Access

    PROCEEDINGS

    Parameter Identification of Biphasic Hyperelastic Constitutive Model with Osmotic Pressure Based on VFM

    Ruike Shi1, Haitian Yang1, Yue Mei1, Yiqian He1,2,*

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.31, No.3, pp. 1-1, 2024, DOI:10.32604/icces.2024.012458

    Abstract Soft biological tissues, like cartilage or arteries, are often modeled as biphasic, considering both solid matrix and interstitial fluid [1]. This biphasic behavior involves chemo-mechanical couplings that control interstitial fluid osmotic pressure [2]. Therefore, the research on the inverse problems of osmotic pressure in soft tissues is important. In this paper, the authors propose a virtual fields method (VFM) for identifying the constitutive model of solid-liquid biphasic hyperelasticity. This method constructs virtual fields based on finite elements (FE) to solve linearly independent virtual fields that can automatically satisfy constraint conditions of the solution of VFM.… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification of ALDH Gene Family under Salt and Drought Stress in Phaseolus vulgaris

    Abdil Hakan Eren*

    Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2883-2907, 2024, DOI:10.32604/phyton.2024.058627 - 30 November 2024

    Abstract Background: Aldehyde dehydrogenase (ALDH) genes constitute an important family of supergenes that play key roles in synthesizing various biomolecules and maintaining cellular homeostasis by catalyzing the oxidation of aldehyde products. With climate change increasing the exposure of plants to abiotic stresses such as salt and drought, ALDH genes have been identified as important contributors to stress tolerance. In particular, they help to reduce stress-induced lipid peroxidation. Objectives: This study aims to identify and characterize members of the ALDH supergene family in Phaseolus vulgaris through a genome-wide bioinformatic analysis and investigate their role in response to abiotic stressors such… More >

  • Open Access

    ARTICLE

    Systematic Identification of Acer rubrum bZIP Transcription Factors and Their Potential Role in Anthocyanin Accumulation under Low Temperature with Light

    Yue Zhao1,2,#, Shah Faheem Afzal2,#, Zhu Chen2, Khan Arif Kamal1,2, Yuzhi Fei2, Xin Meng1,2, Jie Ren2,*, Hua Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 3109-3130, 2024, DOI:10.32604/phyton.2024.056548 - 30 November 2024

    Abstract Acer rubrum is an important garden color-leafed plant. Its leaves will turn red in autumn, which is of great ornamental value. The leaf color change in Acer rubrum is closely associated with anthocyanins accumulation. In anthocyanin synthesis and accumulation, various transcription factor families play significant regulatory roles, including the basic (region) leucine zipper (bZIP). However, there is no report on the systematic identification and functional analysis of the bZIPs in Acer rubrum. In this study, 137 bZIPs distributed on 29 chromosomes of Acer rubrum were identified and renamed according to their locations on the chromosomes. According to the constructed… More >

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