Minmin Xiao¹, Moxiang Cheng¹, Shuangquan Xie¹, Xiushuang Wang¹, Xingming Hao², Li Zhuang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1841-1857, 2023, DOI:10.32604/phyton.2023.027369

Abstract To clarify the characteristics of photoinhibition and the primary defense mechanisms of ephemeral plant leaves against photodestruction under high temperature stress, inhibitors and the technology to determine chlorophyll fluorescence were used to explore the protective effects of D1 protein turnover and the lutein cycle in the high temperature stress of the leaves of three ephemeral plants. The results showed that the maximum light conversion efficiency (Fv/Fm) of the ephemeral plant leaves decreased, and the initial fluorescence (Fo) increased under 35°C ± 1°C heat stress for 1–4 h or on sunny days in the summer. Both Fv/Fm and Fo could be… More >

FANBIAO KONG^1,2,#, KUN WU^3,4,#, LIMING PANG^2,#, YULIANG HUANG^2,#, LEI LI³, JING XU², FEITONG LI², YAN QING², ZHONGYU WANG², XIURONG HUANG³, SHENG XU², XIAOGANG ZHONG², ZHOU ZHU², XIAOTONG WANG^3,*, JIANRONG YANG^5,*

Oncology Research, Vol.30, No.6, pp. 277-288, 2022, DOI:10.32604/or.2022.027301

Abstract Introduction: Siva-1, as a pro-apoptotic protein, has been shown to induce extensive apoptosis in a number of different cell lines. In our previous study, we showed that overexpressed Siva-1 decreased the apoptosis of gastric cancer cells. So, we believe that it can also work as an anti-apoptotic protein. The present study aimed to determine the specific role of Siva-1 in anticancer drug resistance in gastric cancer in vivo and in vitro and preliminarily reveal the mechanism. Materials and Methods: A vincristine-resistant MKN-28/VCR gastric cancer cell line with stably downregulated Siva-1 was established. The effect of Siva-1 downregulation on chemotherapeutic drug… More >

Yu Xia¹, Jian Guan⁴, Yong Gao⁵, Shanfa Tang^1,2,3,*, Jialuo Rong¹

FDMP-Fluid Dynamics & Materials Processing, Vol.19, No.6, pp. 1609-1622, 2023, DOI:10.32604/fdmp.2023.025263

Abstract The ash mudstone in some oil formations is highly water-sensitive. The oil formation is fractured, and the risk of well leakage and collapse is not negligible. This study presents a countermeasure for well collapse prevention, based on a “force-chemistry synergistic balance” approach and the utilization of environmentally friendly and efficient hydration inhibitors. The relevance of this approach is demonstrated considering a drilling fluid system with the high potassium content. The analysis shows that the system can maintain good rheological properties, filtration loss and suspension stability even after aging at 130°C for 16 h. The primary roll recovery of rock chips… More >

MIAOMIAO LI^1,#, DANYANG CHEN^2,#, JUNYI KE¹, RUILIN ZHENG², JINGYAO SU², ZILIN ZHENG¹, JIEYI LUO¹, HANRAN MAI¹, FAN JIANG¹, YANXIA QU¹, XIAOQIONG GU¹, BING ZHU², YINGHUA LI^2,*, LIANDONG ZUO^1,*

BIOCELL, Vol.47, No.2, pp. 401-408, 2023, DOI:10.32604/biocell.2023.025154

Abstract A H₂O₂-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nano-selenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of selenium-containing antioxidant enzymes induced by oxidative stress. A safe and effective nano-drug system of functionalized selenium-containing nanoparticles (SeNPs) was developed with lentinan (LNT) (SeNPs@LNT). Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h to evaluate the cytotoxicity of selenium. GC2-spg cells were randomly divided into the following groups: control, hydrogen peroxide (H₂O₂), SeNPs@LNT, and H₂O₂+SeNPs@LNT groups. H₂O₂+SeNPs@LNT group was… More >

SANHUA LI^1,2,#, QINGHONG KONG^1,2,#, XIAOKE ZHANG^1,2, XINTING ZHU^1,3, CHUNBO YU³, CHANGYAN YU^1,2, NIAN JIANG^1,2, JING HUI^1,2, LINGJIE MENG^1,2,*, YUN LIU^1,2,3,*

BIOCELL, Vol.46, No.11, pp. 2425-2432, 2022, DOI:10.32604/biocell.2022.019916

Abstract Traditional Chinese medicine (TCM) has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers. 3-epi-bufotalin is an active ingredient of TCM “Chanpi” with anti-tumor potential. However, the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed. The present study demonstrated that 3-epi-bufotalin could reduce viability, trigger apoptosis, and block the cell cycle at the G₂/M stage in colorectal cancer cell lines HT29, RKO, and COLO205 in vitro. Moreover, 3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway. These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. More >

Ming Zhang, Baochang Shi, Kai Zhang

Oncology Research, Vol.27, No.9, pp. 1061-1068, 2019, DOI:10.3727/096504019X15565325878380

Abstract Deregulation of miR-186 and Twist1 has been identified to be involved in the progression of multiple cancers. However, the detailed molecular mechanisms underlying miR-186-involved cholangiocarcinoma (CCA) are still unknown. In this study, we found that miR-186 was downregulated in CCA tissues and cell lines, and negatively correlated with the expression of Twist1 protein. In vitro assays demonstrated that miR-186 mimics repressed cell proliferation, in vivo tumor formation, and caused cell cycle arrest. miR-186 mimics also inhibited the migration and invasion of CCLP1 and SG-231 cells. Mechanistically, the 3′-untranslated region (3′-UTR) of Twist1 mRNA is a direct target of miR-186. Further,… More >

Jie Xu^*1, Zhongzhou Su^*1, Qiuping Ding^†, Liang Shen^*, Xiaohu Nie^*, Xuyan Pan^*, Ai Yan^*, Renfu Yan^*, Yue Zhou^*, Liqin Li^‡, Bin Lu^*

Oncology Research, Vol.27, No.7, pp. 819-826, 2019, DOI:10.3727/096504018X15478559215014

Abstract Human glioblastoma multiforme (GBM) accounts for the majority of human brain gliomas. Several TMEM proteins, such as TMEM 45A, TMEM 97, and TMEM 140, are implicated in human brain gliomas. However, the roles of TMEM168 in human GBM remain poorly understood. Herein we found that mRNA levels of TMEM168 were overexpressed in GBM patients (n=85) when compared with healthy people (n=10), which was also supported by data from The Cancer Genome Atlas (TCGA). Kaplan–Meier analysis of Gene Expression Omnibus dataset GSE16011 suggested that enhanced TMEM168 expression was associated with shorter survival time. To investigate whether and how TMEM168 functioned in… More >

Qiuying Quan^*1, Fengyun Zhong^†1, Xinwei Wang^‡, Kai Chen^§, Lingchuan Guo^*

Oncology Research, Vol.27, No.7, pp. 779-788, 2019, DOI:10.3727/096504018X15442985680348

Abstract The aim of this study was to investigate the underlying mechanisms that transforming growth factor- (TGF- )-mediated epithelial-to-mesenchymal transition (EMT) in tumor cells contributes to 5-FU resistance. A series of experiments involving cell viability and caspase activity analyses, siRNA transfection, RNA isolation, and quantitative-PCR (qPCR) assay, cell migration analysis, Western blotting analysis of total protein and membrane protein were performed in this study. Mouse xenograft model was used to determine the effect of the PAR2 inhibitor in vivo. In this study, we found that protease-activated receptor 2 (PAR2) induction in 5-FU therapy is correlated with TGF- -mediated EMT and apoptosis… More >

Yan Gao, Yongchuan Xu, Jue Wang, Xue Yang, Lulu Wen, Juan Feng

Oncology Research, Vol.27, No.3, pp. 341-347, 2019, DOI:10.3727/096504018X15228909735079

Abstract Long noncoding RNAs (lncRNAs) have been acknowledged as important regulators in various human cancers. lncRNA MNX1-AS1 has been shown to be an oncogene in epithelial ovarian cancer. However, the function of MNX1-AS1 in glioblastoma (GBM) remains largely unknown. Here we found that the expression of MNX1-AS1 was significantly upregulated in GBM tissues and cell lines. Knockdown of MNX1-AS1 significantly inhibited the proliferation, migration, and invasion of GBM cells. In terms of mechanism, we found that MNX1-AS1 could bind to miR-4443 in GBM cells. Overexpression of miR-4443 significantly inhibited the expression of MNX1-AS1 and vice versa. Moreover, there was an inverse… More >

Xiao-hui Sun^*, Wen-jie Fan^†, Zong-jian An^‡, Yong Sun^‡

Oncology Research, Vol.28, No.1, pp. 95-102, 2020, DOI:10.3727/096504019X15742472027401

Abstract Long noncoding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including medulloblastoma. However, the functions and molecular mechanisms of CRNDE to the acquired drug resistance of medulloblastoma are still unclear. The transcript levels of CRNDE were examined in four medulloblastoma cell lines exposed to cisplatin treatment, and IC₅₀ values were calculated. Effects of CRNDE knockdown or miR-29c-3p overexpression on cell viability, colony formation, apoptosis, migration, and invasion were assessed using the CCK-8, colony formation assay, flow cytometry, and Transwell assays, respectively. RNA pulldown and RNA-binding protein immunoprecipitation (RIP) were performed to confirm the molecular interactions between… More >