ROBERTO YUNES, PEDRO FERNÁNDEZ, GUSTAVO F. DONCEL, ANÍBAL A. ACOSTA

BIOCELL, Vol.29, No.3, pp. 287-293, 2005, DOI:10.32604/biocell.2005.29.287

Abstract Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was highly conspicuous in both types… More >

G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI

BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209

Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed… More >

Méndez-Trujillo V¹, M Carrillo-Beltrán¹, B Valdez-Salas¹, D Gonzalez-Mendoza²

Phyton-International Journal of Experimental Botany, Vol.85, pp. 225-230, 2016, DOI:10.32604/phyton.2016.85.225

Abstract The aim of the present study was to isolate autochthonous microorganisms with biosurfactants capacities from the rhizospere of the cotton (Gossypium spp.), cachanilla (Pluchea sericea) and salicornia (Salicornia bigelovii) in the Mexicali valley. The biosurfactant activity and biosurfactant productions by the strains isolated from the rhizosphere of the cotton (Bs-Alg), cachanilla (Bs-Cach) and salicornia (Bs-Cach01) were determined using oil spreading technique and emulsification activity with corn, olive, soybean and diesel oils, respectively. The analysis of the 16S rRNA showed that strains Bs-Alg, Bs-Cach and Bs-Cach01 are closely related among them and with respect to all Bacillus subtilis with more than… More >

Gul B¹, M Islam², S Ahmad³, S Gul¹

Phyton-International Journal of Experimental Botany, Vol.85, pp. 94-99, 2016, DOI:10.32604/phyton.2016.85.094

Abstract Spatial and temporal patterns of aboveground biomass and nutritive value of rangeland species with respect to the influence of grazing and soil moisture were investigated. The research was conducted during two years at the Tomagh Research Station, near Sanjawi, Ziarat District, Balochistan, Pakistan. This area is protected from grazing since 1998; however, some of the area is open for grazing. Three sites were selected for research purposes: a protected plain, a protected hilly, and an unprotected plain grazed area. Sampling was carried out during the spring, summer and autumn seasons. Results revealed that soil moisture was greater at the protected… More >

Siliquini OA¹, GA Orioli², JC Lobartini²

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 338-344, 2015, DOI:10.32604/phyton.2015.84.338

Abstract Onion (Allium cepa) is an important horticulture crop because of its value as a food with a long shelf life being a relatively non-perishable product. It is very helpful to understand the growth response of the seeded onion crop to conduct appropriate field practices in attaining the highest or optimum yields. A three year field experiment was conducted using a variety of onion Valcatorce INTA, in a randomized block design with five replicates. Treatments were two plant densities and three rates of N application. The bulb growth followed a classical sigmoid curve. During the rapid growing period, the crop had… More >

Adams RH, AL Álvarez-Ovando, G Castañón N

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 222-232, 2015, DOI:10.32604/phyton.2015.84.222

Abstract In this study, the relationship between the concentration of extra-heavy crude petroleum in a clayey material and the toxicity, field capacity, temperature, and growth of a tropical forage grass (Brachiara humidicola) was determined empirically. For this type of petroleum the acute toxicity (Microtox^®) was slight (CE₅₀ = 63200 - 76400 mg/kg) even at high hydrocarbon concentrations (29279 mg/kg). Nonetheless, serious impacts were encountered in terms of an increase in soil temperature (+ 1.3 °C), reduction in field capacity (-10.7%) and reduction in aerial biomass (-97%). The relationship between hydrocarbon concentration and biomass resulted in a typical dose-response curve (r =… More >

López-Calderón MJ¹, U Figueroa-Viramontes^2*, M Fortis-Hernández¹, G Núñez-Hernández², E Ochoa-Martínez², JI Sanchez-Duarte²

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 8-13, 2015, DOI:10.32604/phyton.2015.84.008

Abstract An experiment with increasing rates of fertilizer and manure in silage corn was established to evaluate the agronomic crop response and to estimate the manure nitrogen availability. The treatments were designed to deliver 0, 67, 100 and 133% of the crop nitrogen requirements (CNR), using ammonium sulphate and manure as N source. Dry matter (DM) yield was similar among treatments receiving N, but those values were greater than those found in the control. Nitrogen extraction at harvest was not statistically different in treatments with fertilizer or manure, but it was higher in these treatments than in the control without N… More >

Gul B¹, M Islam², S Ahmad³, S Gul¹

Phyton-International Journal of Experimental Botany, Vol.83, pp. 415-421, 2014, DOI:10.32604/phyton.2014.83.415

Abstract The effect of fire on growth and nutritive value of Saccharum griffithii Munro ex Boiss-ravennae (Linn.) Murr. hybrid, a low palatable perennial grass in the semi-arid region of the highlands of Balochistan, Pakistan, was evaluated during 2008 and 2009. Prescribed fire treatments were applied in February 2008 and 2009 at the dormant stage of vegetation. Different growth parameters (tiller height, tiller number/plant), forage production (fresh, dry) and nutritive value (crude protein, ash) were compared with control (unburnt) plots. Fire increased the number of tillers, and the fresh and dry forage production in both years. Fire also improved the crude protein… More >

Qian J^1,2, Z Wang¹, Z Liu¹, W Kuang^1,2, CA Busso³

Phyton-International Journal of Experimental Botany, Vol.83, pp. 407-414, 2014, DOI:10.32604/phyton.2014.83.407

Abstract Understanding the changes in the total bud bank, and its contribution to community regeneration, in response to grassland enclosure to livestock grazing, is crucial for grassland management. Despite its importance, the contribution of the total bud bank and that of different bud types to community regeneration as a whole have been rarely explored. The vegetative offspring recruited from different bud types was investigated in grasslands having different enclosure durations to livestock grazing on a typical steppe of Inner Mongolia, China. Total vegetative offspring density was significantly higher (p<0.05) under continuous grazing than in fenced grasslands, but no significant changes were… More >

Morales-Pérez E¹, EJ Morales-Rosales², O Franco-Mora², D de Jesús Pérez-López², A González-Huerta², E Urbina Sánchez³

Phyton-International Journal of Experimental Botany, Vol.83, pp. 333-340, 2014, DOI:10.32604/phyton.2014.83.333

Abstract The aim of this study was to assess different doses of gibberellic and salicylic acids for the production of flowers on Gerbera jamesonni after applications to their foliage or substrate under greenhouse conditions. The ten treatments (growth regulators) were evaluated in a 10 x 2 factorial experiment in a randomized complete block design with four replications. When F values were significant, the honestly significant difference test (HSD) at the 5% level of significance was used. The principal components analysis was also used to study the interrelationship among the ten treatments and the two forms of application (foliar or substrate). The… More >