GANG WANG^#, MINGPING QIAN^#, WEI JIAN, JUHANG CHU, YIXIANG HUANG^*

Oncology Research, Vol.31, No.1, pp. 63-70, 2023, DOI:10.32604/or.2022.028168 - 01 March 2023

Abstract Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction, has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069… More >

YONGDAE YOON^1,#, SOONJAE HWANG^1,2,#, FATEMA TUJ SAIMA^3,#, MOON YOUNG KIM^1,3,4, SOON KOO BAIK^1,3,4,*, YOUNG WOO EOM^1,3,*

BIOCELL, Vol.47, No.3, pp. 669-676, 2023, DOI:10.32604/biocell.2023.025365 - 03 January 2023

Abstract Background: Activated hepatic stellate cells (HSCs) are closely involved in the initiation, perpetuation, and resolution of liver fibrosis. Pro-inflammatory cytokine levels are positively correlated with the transition from liver injury to fibrogenesis and contribute to HSC pathophysiology in liver fibrosis. Methods: In this study, we investigated the effect of the pro-inflammatory cytokine interleukin (IL)-1β on the proliferation and signaling pathways involved in fibrogenesis in LX-2 cells, an HSC cell line, using western blotting and cell proliferation assays. Results: IL-1β increased the proliferation rate and α-smooth muscle actin (SMA) expression of LX-2 cells in a dose-dependent manner. Within… More >

NA HU^1,2,#, SHIQING ZHANG^2,3,#, AQUAN JIN², LIANYING GUO², ZHENYUN QU², JUN WANG^2,*

Oncology Research, Vol.30, No.2, pp. 89-97, 2022, DOI:10.32604/or.2022.027058 - 05 January 2023

Abstract We aimed to explore the molecular mechanism that were involved in SPINK1-induced proliferation and clonogenic survival of human colorectal carcinoma (CRC) HT29 cells. Initially, we generated HT29 cells either permanently silencing or overexpressing SPINK1 protein. The results showed that SPINK1 overexpression (OE) significantly stimulated the proliferation and clonal formation of HT29 cells at the varied time points. Secondly, we found SPINK1 OE enhanced the ratio of LC3II/LC3I and the level of autophagy-related gene 5 (ATG5), whereas SPINK1 knockdown (Kd) reversed the above outcome under normal culturing and/or fasting condition in the cells, indicating its role… More >

Linpei Wang^1,#, Xiaoqiu Ma^2,#, Youqi Chen¹, Jiahui Zhang¹, Jiawei Zhang¹, Wei Wang^1,*, Shaojian Chen^3,*

Oncologie, Vol.24, No.4, pp. 897-912, 2022, DOI:10.32604/oncologie.2022.025693 - 31 December 2022

Abstract Background: MicroRNA-145-5p (miR-145-5p) reportedly inhibits hepatocellular carcinoma (HCC) by targeting ARF6, SPATS2, CDCA3, KLF5, and NRAS, indicating that miR-145-5p plays an important role in the occurrence and development of HCC by regulating the expression of various genes. In this study, we aimed to explore novel downstream targets of miR-145-5p and elucidate the potential mechanism of miR-145-5p in HCC. Materials and Methods: A bioinformatics analysis was performed to determine the clinical significance of miR-145-5p and alpha/beta hydrolase domain-containing protein 17C (ABHD17C) in patients with HCC. The ability of Hep3B cells to proliferate, migrate, and invade was examined after… More >

Shengjin Yu, Huiming Lv, Jinhui Zhang, He Zhang, Weiwei Ju, Yu Jiang^*, Lijuan Lin^*

Oncologie, Vol.24, No.3, pp. 539-551, 2022, DOI:10.32604/oncologie.2022.024882 - 19 September 2022

Abstract Background: Although heparanase/syndecan-1 axis is involved in malignant progression of many cancers, its significance in liver cancer is not well understood. In this study, we explored the value of heparanase/syndecan-1 axis expression in liver cancer and the intervention mechanisms that target this axis by inhibiting the proliferation of hepatocellular carcinoma cells. Materials and Methods: We conducted tissue microarray analysis that included 90 primary liver cancer and their corresponding adjacent samples to evaluate the expression of heparanase and syndecan-1 and their correlation with clinicopathologic characteristics. RNA interference and western blot assays were performed to analyze the effect of… More >

SANHUA LI^1,2,#, QINGHONG KONG^1,2,#, XIAOKE ZHANG^1,2, XINTING ZHU^1,3, CHUNBO YU³, CHANGYAN YU^1,2, NIAN JIANG^1,2, JING HUI^1,2, LINGJIE MENG^1,2,*, YUN LIU^1,2,3,*

BIOCELL, Vol.46, No.11, pp. 2425-2432, 2022, DOI:10.32604/biocell.2022.019916 - 07 July 2022

Abstract Traditional Chinese medicine (TCM) has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers. 3-epi-bufotalin is an active ingredient of TCM “Chanpi” with anti-tumor potential. However, the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed. The present study demonstrated that 3-epi-bufotalin could reduce viability, trigger apoptosis, and block the cell cycle at the G₂/M stage in colorectal cancer cell lines HT29, RKO, and COLO205 in vitro. Moreover, 3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway. These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. More >

MENGMENG LIU^1,#, YUE PAN^1,2,#, XUFENG TAO¹, WENLI KANG¹, YINGJIE LIU¹, YONGJIE YANG^3,4,*, GARY GUISHAN XIAO^1,*

BIOCELL, Vol.46, No.10, pp. 2257-2265, 2022, DOI:10.32604/biocell.2022.020325 - 13 June 2022

Abstract Pancreatic ductal adenocarcinoma (PDAC) is universally acknowledged as the cancer with the highest mortality rate. Berberine has high medicinal value and has been used as an anti-cancer agent. Hence the purpose of this study was to investigate the anti-cancer effect of berberine in PDAC. Berberine was shown to have a selective anti-cancer effect on PDAC by MTT assay in vitro. Pancreatic cancer stem cells (PCSCs), regulated by epithelial–mesenchymal transition (EMT), could promote the proliferation of PDAC cells. However, berberine suppressed the proliferation and stemness of PCSCs through immunofluorescence staining, stem cell sphere assays and so forth in More >

ZHIYONG ZHANG^1,#, YAN PAN^2,#, YAN ZHAO¹, MUDAN REN¹, YARUI LI¹, YUN FENG¹, GUIFANG LU^1,*, SHUIXIANG HE^1,*

BIOCELL, Vol.46, No.8, pp. 1917-1924, 2022, DOI:10.32604/biocell.2022.018847 - 22 April 2022

Abstract Colorectal cancer (CRC) is a heterogeneous cancer, and many risk factors for colorectal cancer have been established. For CRC metastasis, tumor cell migration, adhesion as well as invasion are important processes. WiskottAldrich syndrome protein family member 3 (WASF3) is necessary for metastasis of various types of cancers. However, its role in CRC progression has not been fully elucidated. This study examined the in vitro functional roles of WASF3 in the CRC and explored the underlying molecular mechanisms. We used siRNA-WASF3 to gene silence WASF3 in colon cancer cell (HCT116) in vitro. The effects of WASF3… More >

Elsa Maitre, Xavier Troussard^*

Oncologie, Vol.24, No.1, pp. 3-24, 2022, DOI:10.32604/oncologie.2022.021490 - 31 March 2022

Abstract La leucémie à tricholeucocytes (LT) représente 2% de l’ensemble des leucémies. Le diagnostic repose sur la présence dans le sang et/ou la moelle de tricholeucocytes: cellules lymphoïdes B au cytoplasme chevelu exprimant le CD103, CD123, CD11c et CD25. La mutation BRAF^V600E, marqueur moléculaire de la maladie, est présente dans plus de 80% des cas. La LT doit être distinguée des autres syndromes lymphoprolifératifs chroniques B, notamment des autres proliférations à cellules chevelues, forme variante de la leucémie à tricholeucocytes (LT-V) et lymphome splé- nique diffus de la pulpe rouge (LSDPR). Des progrès thérapeutiques ont été récemment… More >

Yi Zheng¹, Youyou Wang², Chen Zou¹, Bicheng Hu², Min Zhao², Xinxing Wu^2,*

Oncologie, Vol.24, No.1, pp. 147-161, 2022, DOI:10.32604/oncologie.2022.019236 - 31 March 2022

Abstract Tumor-associated macrophages (TAMs) are important components in tumor microenvironment. This study intended to explore the influence of TAMs on cervical cancer cells proliferation and migration. The expression levels of TAMs markers, CD68 and CD163, in tissues were examined by immunohistochemistry and increased with the progression of cervical lesions (p < 0.05). TAMs with M2-like phenotype (PMA(Polymethacrylate) induced THP-1 cells) were noticed to promote the proliferation of cervical cancer cells and improve the migration ability of tumor cells. These enhancements were attributed to secreting soluble components and the physical contact between macrophages and tumor cells. The tumor More >