NA HU^1,2,#, SHIQING ZHANG^2,3,#, AQUAN JIN², LIANYING GUO², ZHENYUN QU², JUN WANG^2,*

Oncology Research, Vol.30, No.2, pp. 89-97, 2022, DOI:10.32604/or.2022.027058

Abstract We aimed to explore the molecular mechanism that were involved in SPINK1-induced proliferation and clonogenic survival of human colorectal carcinoma (CRC) HT29 cells. Initially, we generated HT29 cells either permanently silencing or overexpressing SPINK1 protein. The results showed that SPINK1 overexpression (OE) significantly stimulated the proliferation and clonal formation of HT29 cells at the varied time points. Secondly, we found SPINK1 OE enhanced the ratio of LC3II/LC3I and the level of autophagy-related gene 5 (ATG5), whereas SPINK1 knockdown (Kd) reversed the above outcome under normal culturing and/or fasting condition in the cells, indicating its role in autophagy enhancement. Moreover, LC3-GFP-transfected… More >

YONGDAE YOON^1,#, SOONJAE HWANG^1,2,#, FATEMA TUJ SAIMA^3,#, MOON YOUNG KIM^1,3,4, SOON KOO BAIK^1,3,4,*, YOUNG WOO EOM^1,3,*

BIOCELL, Vol.47, No.3, pp. 669-676, 2023, DOI:10.32604/biocell.2023.025365

Abstract Background: Activated hepatic stellate cells (HSCs) are closely involved in the initiation, perpetuation, and resolution of liver fibrosis. Pro-inflammatory cytokine levels are positively correlated with the transition from liver injury to fibrogenesis and contribute to HSC pathophysiology in liver fibrosis. Methods: In this study, we investigated the effect of the pro-inflammatory cytokine interleukin (IL)-1β on the proliferation and signaling pathways involved in fibrogenesis in LX-2 cells, an HSC cell line, using western blotting and cell proliferation assays. Results: IL-1β increased the proliferation rate and α-smooth muscle actin (SMA) expression of LX-2 cells in a dose-dependent manner. Within 1 h after… More >

Linpei Wang^1,#, Xiaoqiu Ma^2,#, Youqi Chen¹, Jiahui Zhang¹, Jiawei Zhang¹, Wei Wang^1,*, Shaojian Chen^3,*

Oncologie, Vol.24, No.4, pp. 897-912, 2022, DOI:10.32604/oncologie.2022.025693

Abstract Background: MicroRNA-145-5p (miR-145-5p) reportedly inhibits hepatocellular carcinoma (HCC) by targeting ARF6, SPATS2, CDCA3, KLF5, and NRAS, indicating that miR-145-5p plays an important role in the occurrence and development of HCC by regulating the expression of various genes. In this study, we aimed to explore novel downstream targets of miR-145-5p and elucidate the potential mechanism of miR-145-5p in HCC. Materials and Methods: A bioinformatics analysis was performed to determine the clinical significance of miR-145-5p and alpha/beta hydrolase domain-containing protein 17C (ABHD17C) in patients with HCC. The ability of Hep3B cells to proliferate, migrate, and invade was examined after overexpression of miR-145-5p… More >

Shengjin Yu, Huiming Lv, Jinhui Zhang, He Zhang, Weiwei Ju, Yu Jiang^*, Lijuan Lin^*

Oncologie, Vol.24, No.3, pp. 539-551, 2022, DOI:10.32604/oncologie.2022.024882

Abstract Background: Although heparanase/syndecan-1 axis is involved in malignant progression of many cancers, its significance in liver cancer is not well understood. In this study, we explored the value of heparanase/syndecan-1 axis expression in liver cancer and the intervention mechanisms that target this axis by inhibiting the proliferation of hepatocellular carcinoma cells. Materials and Methods: We conducted tissue microarray analysis that included 90 primary liver cancer and their corresponding adjacent samples to evaluate the expression of heparanase and syndecan-1 and their correlation with clinicopathologic characteristics. RNA interference and western blot assays were performed to analyze the effect of heparanase on syndecan-1… More >

HUAMIN WANG^1,#, YANTING WU^1,#, ZHENLEI WANG^2,#, YUHANG CHEN¹, JINYU MO¹, WEN GUAN¹, YALI ZHANG¹, HONGLIANG YAO^1,*

Oncology Research, Vol.29, No.3, pp. 201-215, 2021, DOI:10.32604/or.2022.03553

Abstract LncRNAs and metabolism represents two factors involved in cancer initiation and progression. However, the interaction between lncRNAs and metabolism remains to be fully explored. In this study, lncRNA FEZF1-AS1 (FEZF1- AS1) was found upregulated in colon cancer after screening all the lncRNAs of colon cancer tissues deposited in TCGA, the result of which was further confirmed by RNAscope staining on a colon tissue chip. The results obtained using FEZF1- AS1 knockout colon cancer cells (SW480 KO and HCT-116 KO) constructed using CRISPR/Cas9 system confirmed the proliferation, invasion, and migration-promoting function of FEZF1-AS1 in vitro. Mechanistically, FEZF1-AS1 associated with the mitochondrial… More >

TAO LIU¹, LIMIN ZHOU¹, LIANBO ZHANG², XIN GUAN³, YI DONG^1,*

Oncology Research, Vol.29, No.3, pp. 189-200, 2021, DOI:10.32604/or.2022.03573

Abstract Many studies have illustrated the significance of long noncoding RNAs in oncogenesis and promotion of breast cancer (BC). However, the biological roles of CCDC183 antisense RNA 1 (CCDC183-AS1) in BC have rarely been characterized. Thus, we explored whether CCDC183-AS1 is involved in the malignancy of BC and elucidated the possible underlying mechanisms. Our data confirmed elevated CCDC183-AS1 expression in BC, which was associated with poor clinical outcomes. Functionally, knocking down CCDC183-AS1 hampered cell proliferation, colony formation, migration, and invasion in BC. Additionally, the absence of CCDC183-AS1 restrained tumor growth in vivo. Mechanistically, CCDC183-AS1 executed as a competitive endogenous RNA in… More >

LEI SUN^1,2, YUE ZHANG³, YUQIN YAO⁴, HONGLIN DU³, YUEHUA ZHANG¹, AIPING FANG^1,2,*

Oncology Research, Vol.29, No.3, pp. 175-187, 2021, DOI:10.32604/or.2022.03550

Abstract Long intergenic non-protein coding RNA 1124 (LINC01124) has been identified as an important regulator of non-small-cell lung cancer. However, the expression and detailed role of LINC01124 in hepatocellular carcinoma (HCC) remain unestablished to date. Therefore, this study aimed to elucidate the role of LINC01124 in the aggressiveness of HCC cells and identify the underlying regulatory mechanism. Quantitative reverse transcriptase-polymerase chain reaction was performed to measure the expression of LINC01124 in HCC. Cell Counting Kit-8 assay, Transwell cell migration and invasion assays, and a xenograft tumor model were used to investigate the function of LINC01124 in HCC cells, and bioinformatics analysis,… More >

ZHEN JIA^1,2,#, ZHENGTING QIAN^1,2,#, YONG TANG², XIANG LI², YAN SHI², HENG XIN^1,2, YOUWU FAN^1,2,*, HEMING WU^2,*

Oncology Research, Vol.29, No.2, pp. 105-117, 2021, DOI:10.32604/or.2022.03536

Abstract Glioma is a general malignant tumor with a dismal prognosis. Long noncoding RNAs (lncRNAs) have been implicated in the initiation and processes of tumors. An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1 (WEE2-AS1) is upregulated in glioma tissues compared to normal brain tissues, and validation with quantitative real-time polymerase chain reaction (qRT–PCR) revealed that WEE2-AS1 expression was consistent with the database prediction. Fluorescence in situ hybridization (FISH) assays revealed that WEE2-AS1 was localized primarily in the cytoplasm. Clone formation experiment and EDU assay were used to detect cell proliferation ability, and Transwell assay… More >

BIN LIU^1,2,#, HAIPENG LIU^3,#, FEIFEI REN^1,2, HANGFAN LIU¹, IHTISHAM BUKHARI¹, YUMING FU⁴, WANQING WU⁴, MINGHAI ZHAO⁵, SHAOGONG ZHU⁶, HUI MO¹, FAZHAN LI^1,2, MICHAEL B. ZHENG⁷, YOUCAI TANG^1,2, PENGYUAN ZHENG^1,2,*, YANG MI^1,2,*

Oncology Research, Vol.29, No.2, pp. 87-103, 2021, DOI:10.32604/or.2022.03529

Abstract The activation of some oncogenes promote cancer cell proliferation and growth, facilitate cancer progression and metastasis by induce DNA replication stress, even genome instability. Activation of the cyclic GMP-AMP synthase (cGAS) mediates classical DNA sensing, is involved in genome instability, and is linked to various tumor development or therapy. However, the function of cGAS in gastric cancer remains elusive. In this study, the TCGA database and retrospective immunohistochemical analyses revealed substantially high cGAS expression in gastric cancer tissues and cell lines. By employing cGAS high-expression gastric cancer cell lines, including AGS and MKN45, ectopic silencing of cGAS caused a significant… More >

SANHUA LI^1,2,#, QINGHONG KONG^1,2,#, XIAOKE ZHANG^1,2, XINTING ZHU^1,3, CHUNBO YU³, CHANGYAN YU^1,2, NIAN JIANG^1,2, JING HUI^1,2, LINGJIE MENG^1,2,*, YUN LIU^1,2,3,*

BIOCELL, Vol.46, No.11, pp. 2425-2432, 2022, DOI:10.32604/biocell.2022.019916

Abstract Traditional Chinese medicine (TCM) has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers. 3-epi-bufotalin is an active ingredient of TCM “Chanpi” with anti-tumor potential. However, the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed. The present study demonstrated that 3-epi-bufotalin could reduce viability, trigger apoptosis, and block the cell cycle at the G₂/M stage in colorectal cancer cell lines HT29, RKO, and COLO205 in vitro. Moreover, 3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway. These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. More >