Calixto-Muñoz JJ¹, MD Mariezcurrena-Berasain¹, AT Gutiérrez-Ibáñez¹, A Balbuena-Melgarejo¹, S Rajaram², ED Archundia-Velarde¹, DL Martínez-Pardo³, DL Pinzón-Martínez¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 171-177, 2018, DOI:10.32604/phyton.2018.87.171

Abstract Wheat is one of the most important protein sources for human beings. Several food products are elaborated from this cereal, such as bread. Mexican wheat breeding programs are focused on to identify those genotypes with high yields and bread quality. Therefore, the aim of this study was to evaluate the environmental effects over the Physicochemical and Bromatological quality parameters of Cal Blanco F2011, Matchett F2011 and RSM-Norman F2008 wheats sown at the experimental fields at Mexicali, Baja California, Querendaro, Michoacán and Tarimoro, Guanajuato, Mexico during the autumn-winter growing cycle for two consecutive years (2014-2016) under irrigation conditions. Grain Hectolitric weight… More >

LI KAILONG^1,2, DU XIAOLAN¹, HE YANI², ZHAO LIN¹, YANG JVRONG², SONG RUIHUA¹, CHEN LIN¹

BIOCELL, Vol.31, No.2, pp. 213-223, 2007, DOI:10.32604/biocell.2007.31.213

Abstract Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice… More >

KIVANÇ ERGEN^*, MUHAMMET BEKTAŞ^**, SINA GÖKÇE^**, RÜSTEM NURTEN^**

BIOCELL, Vol.31, No.1, pp. 61-66, 2007, DOI:10.32604/biocell.2007.31.061

Abstract Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis.
In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related… More >

M. V. Kurian¹, J. M. Mullins¹, L. R. Hamilton¹, P. M. Mehl², J. K. Keevan²

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 217-218, 2006, DOI:10.32604/mcb.2006.003.217

Abstract This article has no abstract. More >

Min Yue^1,1, Jeanne C. Stachowiak^1,1,2,2, Arunava Majumdar^1,1,3,3

Molecular & Cellular Biomechanics, Vol.1, No.3, pp. 211-220, 2004, DOI:10.3970/mcb.2004.001.211

Abstract Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed. More >

Maximiliano GIRAUD-BILLOUD^1,2,*, Claudio M. FADER^1,3, Rocío AGÜERO², Fernando EZQUER⁴, Marcelo EZQUER⁴

BIOCELL, Vol.42, No.2, pp. 35-40, 2018, DOI:10.32604/biocell.2018.07010

Abstract Diabetic nephropathy (DN) is the most frequent cause of chronic renal failure. Until now, the pathophysiological mechanisms that determine its development and progression have not yet been elucidated. In the present study, we evaluate the role of autophagy at early stages of DN, induced in type 2 diabetes mellitus (T2DM) mouse, and its association with proximal tubule membrane endocytic receptors, megalin and cubilin. In T2DM animals we observed a tubule-interstitial injury with significantly increased levels of urinary GGT and ALP, but an absence of tubulointerstitial fibrosis. Kidney proximal tubule cells of T2DM animals showed autophagic vesicles larger than those observed… More >

Chikit Au¹, Yiyu Cai², Jianmin Zheng³, Tony Woo⁴

CMES-Computer Modeling in Engineering & Sciences, Vol.68, No.3, pp. 221-238, 2010, DOI:10.3970/cmes.2010.068.221

Abstract A protein model (such as a ribbon model) can be created from the atomic coordinates in the protein data base files. These coordinates are obtained by X-ray crystallography or NMR spectroscopy with the protein arbitrarily oriented. As such, identifying or comparing a novel structure with a known item using protein model in the protein data base can be a timely process since a large number of transformations may be involved. The identification efficiency will be improved if the protein models are uniformly oriented. This paper presents an approach to orient a protein model to generate the characteristic views with minimum… More >

Maryam Monazzah¹, Sattar Tahmasebi Enferadi^1,*, Zohre Rabiei¹ and Alessandro Mattiello²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 149-159, 2019, DOI:10.32604/phyton.2019.05053

Abstract Helianthus annuus L. as an oil seed crop is widely grown throughout the world. One of the most destructive diseases of sunflower is stem rot caused by Sclerotinia sclerotiorum. Oxalic acid is the major virulence factor of this necrotrophic pathogen. It is important to further investigate plant responses to this non-specific toxin. Therefore, in the present study, we compared the patterns of total soluble proteins and xylem morphology of partially resistant and susceptible sunflower lines after treatment with Sclerotinia culture filtrate. The basal stems of both lines were treated with 40 mM oxalic acid (pH 3.7) of fungus culture filtrate… More >

Min Huang*, Hengdong Zhang, Chunrong Zhao, Guanghui Chen and Yingbin Zou

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 101-107, 2019, DOI:10.32604/phyton.2019.06694

Abstract Improving grain amino acid content of rice (Oryza sativa L.) is essential for the health of consumers. This study was conducted to identify the physiological processes that contribute to the higher grain amino acid content in hybrid rice cultivar Lingliangyou 268 compared to Luliangyou 996. The results showed that total amino acid content in grains was 9% higher in Lingliangyou 268 than in Luliangyou 996. There was no significant difference in grain nitrogen (N) content between Lingliangyou 268 and Luliangyou 996, while ratio of amino acid to N was 6% higher in Lingliangyou 268 compared to Luliangyou 996. A total… More >

Bengü ERGÜDEN

BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517

Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >