Danni He, Guoli Deng, Songpei Ying, Wenjuan Yang, Jiali Wei, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 633-643, 2020, DOI:10.32604/phyton.2020.010498

Abstract Primary seed dormancy is an adaptive strategy that prevents germination for viable seeds in harsh environment, ensuring seeds germination under favorable condition. Accurately inducing seeds germination in a controllable manner is important for crop production. Thus searching the chemicals that efficiently breaks seed dormancy is valuable. DOG1 protein abundance in the freshly harvested seed is high, and its level is correlated to seed dormancy intensity, thus DOG1 is regarded as the timer to evaluate the seed dormancy degree. In this study, we found the carbon monoxide (CO) donor treatment, the transgenic line with high CO content, showed lower seed dormancy,… More >

Qili Zhang, Danni He, Songbei Ying, Shiyan Lu, Jiali Wei, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 619-631, 2020, DOI:10.32604/phyton.2020.010379

Abstract Seeds germination is strictly controlled by environment factor such as high temperature (HT) through altering the balance between gibberellin acid (GA) and abscisic acid (ABA). Gama-aminobutyric acid (GABA) is a small molecule with four-carbon amino acid, which plays a crucial role during plant physiological process associated with pollination, wounding or abiotic stress, but its role in seeds germination under HT remains elusive. In this study we found that HT induced the overaccumulation of ROS, mainly H₂O₂ and O₂^- , to suppress seeds germination, meanwhile, HT also activated the enzyme activity of GAD for the rapid accumulation of GABA, hinting the… More >

Shuaishuai Zhang¹, Jin Li¹, Yongchang Shen¹, Linda Nartey Korkor¹, Qian Pu¹, Jin Lu¹, Balock Shakeela¹, Dedong Kong², Ou Li¹, Guohong Zeng^1,*, Xiufang Hu^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 599-617, 2020, DOI:10.32604/phyton.2020.010074

Abstract This study aimed to explore the cold tolerance of two cultivars of Dendrobium officinale (MG1, MG2) grown in different regions of China. Under -2°C incubation, cultivar MG1 remained active after 3 d, and continued to grow after returning to room temperature. However, MG2 could only maintain its activity after 2 d treatment at −2°C, and the seedlings died with the low temperature treatment time. Investigation of the characteristics of the plants grown in the south (Hangzhou) or north (Zhengzhou) of China indicated that the leaves of MG1 also had reduced stomatal density, the highest thickness, and a compact microstructure. The… More >

Chongchong Yan¹, Qianqian Wang¹, Nan Zhang², Jiajia Wang¹, Xuexiang Ren¹, Bingjie Xue³, Xiaojing Pu⁴, Zhihong Xu⁴, Huajun Liao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 561-586, 2020, DOI:10.32604/phyton.2020.010322

Abstract Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity… More >

Md. Shakhawat Hossain¹, Md. Daud Hossain¹, Abdul Hannan², Mirza Hasanuzzaman³, Md. Motiar Rohman^4,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 541-559, 2020, DOI:10.32604/phyton.2020.010279

Abstract Salinity stress is a major factor limiting plant growth and productivity of many crops including oilseed. The present study investigated the identification of salt tolerant mustard genotypes and better understanding the mechanism of salinity tolerance. Salt stresses significantly reduced relative water content (RWC), chlorophyll (Chl) content, K⁺ and K⁺ /Na⁺ ratio, photosynthetic rate (P_N), transpiration rate (Tr), stomatal conductance (gs), intercellular CO₂ concentration (Ci) and increased the levels of proline (Pro) and lipid peroxidation (MDA) contents, Na⁺ , superoxide (O₂^•− ) and hydrogen peroxide (H₂O₂) in both tolerant and sensitive mustard genotypes. The tolerant genotypes maintained higher Pro and lower… More >

Daoxi Li^1,*, Ruitao Lou¹, Yanbin Li¹, Zepeng Bian¹, Ya’nan Zhu²

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 691-703, 2020, DOI:10.32604/phyton.2020.010661

Abstract A drought event can cause entire crops to fail or yield loss. In order to study the effects of continuous drought on photosynthetic characteristics, yield, and water use efficiency (WUE) of winter wheat (Triticum aestivum L.), the winter wheat variety “Aikang 58” was selected as test material with controlling the water of the pot-planted winter wheat under a mobile rainout shelter. Based on foot planting and safe wintering, winter wheat was evaluated under different drought conditions, including light, moderate and severe drought at the jointing (B), heading (C), and filling (G) stages. The soil water content was controlled in a… More >

Shuai Li, Jianming Zhou, Shibo Xiang, Songhong Wei^*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 681-690, 2020, DOI:10.32604/phyton.2020.010671

Abstract Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we found a small secreted hypersensitive response-inducing protein UVI_02019870 was highly conserved in fungi. Furthermore, we performed Y2H and BiFC assay to demonstrated UVI_02019870 interacted with OsCPL1, which was predicted as a chloroplast… More >

Thatsanee Luangharn^1,2,3,4, Samantha C. Karunarathna^1,3, Peter E. Mortimer^1,4,*, Kevin D. Hyde^3,5, Jianchu Xu^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 743-764, 2020, DOI:10.32604/phyton.2020.09690

Abstract Ganoderma is a genus of medicinally and economically important mushrooms in the family Ganodermataceae. Ganoderma species are popular medicinal mushrooms and their health benefits are well-documented. Ganoderma is a cosmopolitan genus that is widely distributed in both tropical and temperate regions. This genus is characterized by its unique laccate or non-laccate species with double-walled basidiospores. Here, we report on eight collections of G. gibbosum collected during surveys in Kunming, Yunnan Province, China. The specimens are described and illustrated based on macro- and micro-morphological characteristics. Total DNA of the eight G. gibbosum strains were extracted using the Biospin Fungal Extraction Kit… More >

Gloria Viviana Cerrillo-Rojas¹, Mariana Tiscareño-Andrade¹, Ana Erika Ochoa-Alfaro², Eugenio Pérez-Molphe Balch¹, Ruth Elena Soria-Guerra², José Francisco Morales-Domínguez^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 715-726, 2020, DOI:10.32604/phyton.2020.09769

Abstract Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and, they are suitable for reclamation and remediation of saline soil. We develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis. Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. The highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ±… More >

Ejaz Ahmad Waraich^1,*, Zeeshan Ahmed^2,3, Zahoor Ahmad⁴, Rashid Ahmad¹, Murat Erman⁵, Fatih Cig⁵, Ayman El Sabagh^5,6

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 587-597, 2020, DOI:10.32604/phyton.2020.08734

Abstract Camelina (Camelina sativa L.) is famous for its oil quality and unique fatty acid pattern. Growth and yield of crops reduced under water deficit conditions. Environmental threat such as drought or water deficit condition is the emerging problem which creates the negative impact on the growth of plants. Based upon the current situation a pot study was performed in rain out-shelter to explore the effect of different plant densities (15, 10 and 5 plants per pot) on growth and seed yield of two camelina genotypes under normal (100% WHC) and water deficit (60% WHC) conditions by using completely randomized design… More >