Peng M^1,2, FL Huang^1*, FJ Meng², BZ Hu^3*, XF Chen¹, R Luo⁴, N Li¹, RF Wang¹, Y Zhao¹, QW Zou¹, CT Wu¹, JL Dai⁵

Phyton-International Journal of Experimental Botany, Vol.86, pp. 296-305, 2017, DOI:10.32604/phyton.2017.86.296

Abstract Chinese herbaceous peony (Paeonia lactiflora Pall.) is a popular ornamental plant. However, little is known about its reproductive biology by histological analyses. Here, we used the paraffin wax method to study the reproductive biology of the Chinese herbaceous peony (Paeonia lactiflora ‘Dafugui’). The results showed that the flower bud of Chinese herbaceous peony was a mixed bud. The course of flower bud differentiation included some developmental morphology periods, such as bract, calyx, petal, stamen, and pistil. The anther wall development was related to a dicotyledonous type. Pollen was mainly 2-celled, only few of them were 3-celled. Moreover, the mature pollen… More >

Castañón-Nájera G¹, L Latorunerie-Moreno², YA Gálvez Muñoz³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 290-295, 2017, DOI:10.32604/phyton.2017.86.290

Abstract Among the different types of abiotic stresses, water is the most damaging for crops. In the current research, the effect of water stress, induced with Polyethylene Glycol 8000 (PEG8000) and Sodium chloride (NaCl) solutions, was studied in maize genotypes. A randomized block design (RBD) with a factorial arrangement and four replications of 25 seeds each was used. The studied maize genotypes were RC₂C39, RC₂C51 (wild varieties of the State of Tabasco backcrossed with the endogamic line CML247 of the International Center for the Improvement of Maize and Wheat), wild Tamulte, VS536, and Asgrow 7573 hybrid, study osmotic pressures (OP) were… More >

Ramírez H¹, JC Sánchez-Canseco¹, VM Zamora-Villa², JH Rancaño-Arrioja³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 282-289, 2017, DOI:10.32604/phyton.2017.86.282

Abstract It is well established that the climatic change is altering yield in fruit trees. This adversity reflects, among others, extreme temperature modifications in northern Mexico. This effect modifies negatively the physiology of apple trees (Malus domestica Borkh.), originating excessive shoot growth and a decrease in flower bud formation. In this study, Prohexadione-Ca (P-Ca), 6-benzyl amino purine (BAP) and N6-furfuryladenine (kinetin) were evaluated on shoot and reproductive growth in Golden Delicious apple trees in Arteaga Coah., Mexico. A completely randomized design with 12 treatments was used. These were: Control, Prohexadione calcium (Ca-P) at doses of 150, 300 and 450 mg/L, cytokinins… More >

Díaz-Álvarez EA^1,2, AP Rojas-Cortés^1,2, E de la Barrera¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 278-281, 2017, DOI:10.32604/phyton.2017.86.278

Abstract The epiphytic bromeliad Tillandsia makoyana was studied to assess the contribution of the environmental factors, water, temperature, and light, to the expression of the Crassulacean acid metabolism. In particular, nocturnal titratable acidity accumulation and maximum leaf temperature were measured in response to watering and incident light. Plants that were watered and exposed to direct sunlight had the highest nocturnal accumulation of titratable acidity (25.52 ± 0.34 H⁺/m²), while those without irrigation and shaded showed a 59% reduction in their titratable acidity. Our results illustrated a rapid response of the photosynthetic activity to water availability in Tillandsia makoyana, a native species… More >

Hapon MV^1,2, JJ Boiteux^1,2, MA Fernández¹, G Lucero^1,2, MF Silva¹, PH Pizzuolo^1,2

Phyton-International Journal of Experimental Botany, Vol.86, pp. 270-277, 2017, DOI:10.32604/phyton.2017.86.270

Abstract The polyphagous plant pathogen Botrytis cinerea has the ability of growing fast under several conditions, and producing grey moulds in different plant tissues. Because of its adaptive plasticity, resistant strains have made difficult their chemical control. Therefore, new strategies of management and the use of natural compounds could be important control alternatives. The aim of this work was to study the potential value of four Argentine native plant extracts and some of its phenolic compounds as biodrugs on B. cinerea. Aqueous plant extracts of Larrea divaricata, Prosopis strombulifera, Tessaria absinthioides and Schinus molle var. areira, were used. The capability to… More >

Carmona MA¹, E Simonetti^2,3, ME Ravotti¹, MM Sc,iani⁴, AG Luque⁴, NA Formento⁵, FJ Sautua¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC₅₀) ranged from… More >

García-Munguía CA¹, M Acosta-Ramos², O Vázquez-Martinez³, M Pérez-Sato⁵, A García-Munguía⁴, O García-Munguía⁶, AM García-Munguía³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 258-264, 2017, DOI:10.32604/phyton.2017.86.258

Abstract The illnesses that cause root and neck rot in golf courses constitute a limiting factor for grass development worldwide. This is mainly true when management guidelines are not conducted. Our research was conducted in the Golf Club “Mexico”, located in Mexico, D.F. Isolates were taken from plants that presented yellowing symptoms and root and neck rot. The identified pathogenic fungi, isolated from axenic media, were Giberella avenacea, Fusarium oxysporum, F. oxysporum f. sp. cubense and Pythium ultimum. The molecular characterization showed a comparison of the DNA-obtained sequences which was morphologically determined as F. avenaceum and was molecularly corroborated as G.… More >

edaghathoor S, F Sharifi, A Eslami

Phyton-International Journal of Experimental Botany, Vol.86, pp. 252-257, 2017, DOI:10.32604/phyton.2017.86.252

Abstract The tulip (Tulipa sp.) is an ornamental plant produced by bulbs. Wild tulips are native to several areas of Iran, especially the Alborz and Zagros mountains. Endemic Iranian tulips are the origin of some commercial cultivars of tulip, and mutagens are commonly used to produce new traits and varieties. In this trial, X-rays were applied at 60, 70 and 80 kV; sodium azide (SA) at doses of 0.01%, 0.02%, and 0.03%, and diethyl sulfate (DES) at doses of 0.1%, 0.2% and 0.4 on Kess Nelis and Jan Van Ness varieties of tulip. Morphophysiological traits of the treated plants were measured.… More >

Jin G^1,2, XM Tang³, Y Niu⁴, XY Huang², T Chen², Q Huang², J Zhang², RY Zhou¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 137-142, 2017, DOI:10.32604/phyton.2017.86.137

Abstract In plants, the mitochondrial NADH dehydrogenase (complex I) is a large protein complex transferring electrons to ubiquinone. For the nine chondriogenes encoding complex I subunits (nad1, nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficient strategy for the cloning of complete coding sequences (CDSs) is important. Specific orthologous portions of untranslated regions (UTRs) were found based on multiple sequence alignments of chondriogene orthologues encoding complex I subunits in plant species. Based on the conservation of partial UTRs, a one-step PCR strategy was conceived for the cloning of CDSs of the nine chondriogene orthologues. Using this strategy, the five… More >

Muñoz FF, VE Ruiz, CA Bouzo

Phyton-International Journal of Experimental Botany, Vol.86, pp. 131-136, 2017, DOI:10.32604/phyton.2017.86.131

Abstract The aim of this work was to study the effect of preharvest foliar spraying with calcium nitrate solutions on the fruit melon quality. Treatments included a combination of five solutions with Ca(NO₃)₂ (0.0; 1.3; 2.6; 5.2 and 10.5 g/L) and two postharvest storage environments [(24.0 ± 1.5 °C and 6.4 ± 0,8 mbar of vapor pressure deficit (VPD), and 10.0 ± 0.5 °C and 3.0 ± 0.5 mbar (VPD)]. Fruits stored at 24 °C for 11 days had the lowest water loss with treatments 1.3 and 2.6 g/L Ca (NO₃)₂. It was observed that at both 24 °C and 10… More >