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  • Open Access


    In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures

    Oliver Tusevski1,*, Marija Todorovska1, Jasmina Petreska Stanoeva2, Marina Stefova2, Sonja Gadzovska Simic1

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 921-941, 2023, DOI:10.32604/phyton.2023.024995

    Abstract Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed to… More >

  • Open Access


    Selection of Stable Reference Genes for Quantitative Real-Time PCR on Herbaceous Peony (Paeonia lactiflora Pall.) in Response to Drought Stress

    Zhipeng Sheng, Yuting Luan, Cong Xu, Jun Tao*, Daqiu Zhao*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 801-814, 2023, DOI:10.32604/phyton.2023.024953

    Abstract Herbaceous peony (Paeonia lactiflora Pall.), as a high-end cut flower in the international market, has high ornamental and medicinal values. But in Northern China, drought is a major environmental factor influencing the growth and development of P. lactiflora. Quantitative real-time polymerase chain reaction (qRT-PCR) can evaluate gene expression levels under different stress conditions, and stable internal reference is the key for qRT-PCR. At present, there is no systematic screening of internal reference for correcting gene expressions of P. lactiflora in response to drought stress. In this study, 10 candidate genes [ubiquitin (UBQ2), UBQ1, elongation factor 1-α (EF-1α), Histidine (His), eukaryotic… More >

  • Open Access


    Research Progress and Application of Plant Branching

    Yaru Yang1, Yulan Hu1, Ping Li1, John T. Hancock2, Xiangyang Hu1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 679-689, 2023, DOI:10.32604/phyton.2023.024904

    Abstract Plant branching development plays an important role in plant morphogenesis (aboveground plant type), the number and angle of branches are important agronomic characters that determine crop plant type. Effective branches determine the number of panicles or pods of crops and then control the yield of crops. With the rapid development of plant genomics and molecular genetics, great progress has been made in the study of branching development. In recent years, a series of important branching-related genes have been validated from Arabidopsis thaliana, rice, pea, tomato and maize mutants. It is reviewed that plant branching development is controlled by genetic elements… More >

  • Open Access


    Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus

    Liang Chai1,2, Cheng Cui1, Benchuan Zheng1, Jinfang Zhang1, Jun Jiang1, Haojie Li1,2,*, Liangcai Jiang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 645-663, 2023, DOI:10.32604/phyton.2023.024717

    Abstract Nitrogen (N) is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field. Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive. In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed (Brassica napus L.), comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root, stem and leaf under different N concentrations, respectively. More than 200 differential abundance proteins (DAPs) were detected and categorized into groups according to annotations, including… More >

  • Open Access


    Comparative Analysis of the Photosynthetic Characteristics and Active Compounds of Semiliquidambar cathayensis Chang Heteromorphic Leaves

    Xiaoming Tian*, Guangfeng Xiang, Hao Lv, Jing Peng, Lu Zhu

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 837-850, 2023, DOI:10.32604/phyton.2023.024408

    Abstract In the present study, the variation patterns of leaf shape in different populations of individual Semiliquidambar cathayensis plants were analyzed to investigate the relationship among leaf shape variation, photosynthetic properties, and active compounds to understand the genetic characteristics of S. cathayensis and screen elite germplasms. The leaf shape of 18 offspring from three natural S. cathayensis populations was analyzed to investigate the level of diversity and variation patterns of leaf shape. Furthermore, photosynthetic pigment content, physiological parameters of photosynthesis, and the active compounds in leaves of different shapes were determined. Statistical analysis showed that the leaf shape variation in  S. cathayensisMore >

  • Open Access


    Identification and Characterization of ZF-HD Genes in Response to Abscisic Acid and Abiotic Stresses in Maize

    Xiaojie Jing1,2,3,#, Chunyan Li1,2,3,#, Chengjuan Luo1,2,3, Chaonan Yao1,2,3, Jiahao Zhang1,2,3, Tingting Zhu1,2,3, Jiuguang Wang1,2,3, Chaoxian Liu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 707-723, 2023, DOI:10.32604/phyton.2023.024338

    Abstract The zinc finger homeodomain (ZF-HD) genes belong to the homeobox gene family, playing critical roles in flower development and stress response. Despite their importance, however, to date there has been no genome-wide identification and characterization of the ZF-HD genes that are probably involved in stress responses in maize. In this study, 24 ZF-HD genes were identified, and their chromosomal locations, protein properties, duplication patterns, structures, conserved motifs and expression patterns were investigated. The results revealed that the ZF-HD genes are unevenly distributed on nine chromosomes and that most of these genes lack introns. Six and two ZF-HD genes have undergone… More >

  • Open Access


    Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.

    Yanhua Li1,#, Hualei Huang1,#, Youming Shi1, Shuqin Huang1, Tao Liu1, Changming Xiao1, Xiaoqing Tian2, Ping Zhao1, Xiaoyan Dai3, Taocui Huang1,*, Yan Zhou1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 815-835, 2023, DOI:10.32604/phyton.2023.023928

    Abstract Gibberellin 2-oxidases (GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants. GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in Brassica napus. Here, we identified 31 GA2ox genes in B. napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including… More >

  • Open Access


    How Do Mind-Body Practices Benefit Employees? A Daily Diary Study of Mind–Body Practices after Work and Employees’ Next-Day Proactive Behavior

    Puwen Shang1, Jinfan Zhou2,*, Guanglei Zhang1

    International Journal of Mental Health Promotion, Vol.25, No.1, pp. 45-62, 2023, DOI:10.32604/ijmhp.2023.026606

    Abstract Although it is becoming increasingly popular for organizations to offer programs of mind–body practices to their employees to improve their physical and mental health, the effects that after-work sessions of mind–body practices have on employees’ work behavior remain unclear. Using the model of proactive motivation and experience sampling, this study explored the relationship between employees’ mind–body practices after work and their proactive behavior in the workplace on the following day. A multilevel path analysis of data from 82 employees over seven consecutive workdays showed that employees’ mind–body practices after work had a positive effect on their proactive behavior in the… More >

  • Open Access


    Group Psychological Intervention for Children with Hyperactivity Disorder

    Ying Xu*

    International Journal of Mental Health Promotion, Vol.25, No.1, pp. 81-97, 2023, DOI:10.32604/ijmhp.2022.023720

    Abstract ADHD is a broad psychiatric disorder that affects children of normal or near-normal intelligence. It is characterized by inattention, hyperactivity, and age-inappropriate impulsivity, and it is often accompanied by learning difficulties, behavioral, emotional, and interpersonal problems. On the other hand, hyperactive tendencies in children with ADHD exhibit ADHD-like behaviors such as lack of self-control, inattention, hyperactivity, and emotional impulsivity. However, because their symptoms are less severe, they do not meet the diagnostic criteria for ADHD but are ADHD or at risk of developing ADHD. The purpose of this study is to alleviate and reduce children’s hyperactivity symptoms by investigating the… More >

  • Open Access


    The Early Emotional Responses and Central Issues of People in the Epicenter of the COVID-19 Pandemic: An Analysis from Twitter Text Mining

    Eun-Joo Choi1, Yun-Jung Choi2,*

    International Journal of Mental Health Promotion, Vol.25, No.1, pp. 21-29, 2023, DOI:10.32604/ijmhp.2022.022641

    Abstract This study aimed to explore citizens’ emotional responses and issues of interest in the context of the coronavirus disease 2019 (COVID-19) pandemic. The dataset comprised 65,313 tweets with the location marked as New York State. The data collection period was four days of tweets when New York City imposed a lockdown order due to an increase in confirmed cases. Data analysis was performed using R Studio. The emotional responses in tweets were analyzed using the Bing and NRC (National Research Council Canada) dictionaries. The tweets’ central issue was identified by Text Network Analysis. When tweets were classified as either positive… More > Graphic Abstract

    The Early Emotional Responses and Central Issues of People in the Epicenter of the COVID-19 Pandemic: An Analysis from Twitter Text Mining

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