JU HYUNG LEE¹, IL-KWON KIM^2,3, SANG WOO KIM^2,3, SOYEON LIM^2,3, SEAHYOUNG LEE^2,3, KI-CHUL HWANG^2,3, BYEONG-WOOK SONG^2,3,*

BIOCELL, Vol.46, No.10, pp. 2231-2234, 2022, DOI:10.32604/biocell.2022.020179

Abstract The complex mechanism of degenerative diseases and the non-specific modulation of regenerative targets are topics that need to be elucidated in order to advance the use of stem cells in improvement of neurodegenerative diseases. From pre-transplantation through post-transplantation, there are many changes in the conditions, both inside and outside of the stem cells that have not been carefully considered. This has hindered development in the field of cell therapy and regeneration. This viewpoint highlights the potential implications of intracellular and extracellular alterations of stem cells in transplanted areas at risk of neurodegenerative disease. More >

ISABELLA PANFOLI^1,*, MAURIZIO BRUSCHI², GIOVANNI CANDIANO²

BIOCELL, Vol.46, No.10, pp. 2167-2176, 2022, DOI:10.32604/biocell.2022.020154

Abstract Precision medicine is based on the identification of biomarkers of tumor development and progression. Liquid biopsy is at the forefront of the ability to gather diagnostic and prognostic information on tumors, as it can be noninvasively performed prior or during treatment. Liquid biopsy mostly utilizes circulating tumor cells, or free DNA, but also exosomes. The latter are nanovesicles secreted by most cell types, found in any body fluid that deliver proteins, nucleic acids and lipids to nearby and distant cells with a unique homing ability. Exosomes function in signalling between the tumor microenvironment and the rest of the body, promoting… More >

NADIRE ÖZENVER^1,2, THOMAS EFFERTH^2,*

BIOCELL, Vol.46, No.10, pp. 2151-2157, 2022, DOI:10.32604/biocell.2022.019937

Abstract Carnosic acid (CA) is a natural phenolic diterpene mainly occurring in some species of the Lamiaceae family. Numerous studies described the cytotoxicity of CA towards different types of cancer both in vitro and in vivo. Particularly, the influence of CA in combination with other drugs, vitamins or natural products through affecting various targets has raised interest. Current experimental in vivo data suggested that CA may cooperate with clinically used anticancer drugs promoting their activity against cancer. From this point of view, CA gained importance, because it may alter pharmacodynamic profiles of various agents in the case of their co-administration, and… More >

RéMY GAUTHIER^1,*, HéLèNE FOLLET², ANA-MARIA TRUNFIO-SFARGHIU³, DELPHINE FARLAY², NINA ATTIK^4,5, SYLVAIN MEILLE¹, JéRôME CHEVALIER¹, DAVID MITTON⁶

BIOCELL, Vol.46, No.10, pp. 2209-2216, 2022, DOI:10.32604/biocell.2022.022290

Abstract To develop durable bone healing strategies through improved control of bone repair, it is of critical importance to understand the mechanisms of bone mechanical integrity when in contact with biomaterials and implants. Bone mechanical integrity is defined here as the adaptation of structural properties of remodeled bone in regard to an applied mechanical loading. Accordingly, the authors present why future investigations in bone repair and regeneration should emphasize on the matrix surrounding the osteocytes. Osteocytes are mechanosensitive cells considered as the orchestrators of bone remodeling, which is the biological process involved in bone homeostasis. These bone cells are trapped in… More >

LEI WANG^1,#, QIUXIA DU^1,#, YISU SHI^1,#, MICHAEL ACKAH¹, PENG GUO¹, DANYAN ZHENG¹, MENGMENG WU¹, XIN JIN¹, PEILAN LI³, QIAONAN ZHANG¹, RUIXUE LI⁴, ZHI YIN³, MENGDI ZHAO^2,*, WEIGUO ZHAO^1,*

BIOCELL, Vol.46, No.10, pp. 2327-2342, 2022, DOI:10.32604/biocell.2022.021542

Abstract HMA2 (heavy metal ATPase 2) plays a crucial role in extracellular and intracellular Zn²⁺ transport across biomembranes, maintaining ion homeostasis, and playing an important role in the normal physiological metabolism, growth, and development of plants. In our study, a novel HMA2 gene, named MaHMA2, was isolated and cloned from white mulberry (Morus alba L.). The gene sequence obtained was 1,342 bp long, with an open reading frame of 1,194 bp, encoding a protein of 397 amino acids, with a predicted molecular mass of 42.852 kD and an isoelectric point of 7.53. This protein belonged to the PIB-type ATPase transport protein… More >

MARCO INVERNIZZI^1,2,*, NICOLA FUSCO^3,4,*

BIOCELL, Vol.46, No.10, pp. 2221-2223, 2022, DOI:10.32604/biocell.2022.021043

Abstract This article has no abstract. More >

DONGMEI GAO^1,#, JIAOE CHEN^2,#, HONGZHANG LI^2,*, JUN ZHAO^3,*

BIOCELL, Vol.46, No.10, pp. 2275-2280, 2022, DOI:10.32604/biocell.2022.021008

Abstract Introduction: Human cytomegalovirus (HCMV) is reported to be involved in the occurrence of many human diseases. To further investigate the biological changes of HCMV, we analyzed the relevant factors that affect the autophagy caused by HCMV infection. Methods: Firstly, we cultured human embryonic lung fibroblasts (HELF) cells with HCMV infection, and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay (ELISA), Real-time quantitative Polymerase Chain Reaction (RT-qPCR), Acridine orange (AO) staining and Western blotting (WB) experiments. Results: Through the above experiments, we found that the combined treatment of HCMV infection and carbamazepine, rapamycin and si-mTOR… More >

SHUAI LIU¹, JIANZHONG XU^1,*, TINGSHAN LIU¹, ZHIMING RAO², WEIGUO ZHANG^1,*

BIOCELL, Vol.46, No.10, pp. 2303-2313, 2022, DOI:10.32604/biocell.2022.020899

Abstract In this study, phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas (EMP) pathway and pentose phosphate (PP) pathway in Escherichia coli, thus increasing the L-tryptophan production. Firstly, the effects of disrupting EMP pathway on L-tryptophan production were studied, and the results indicated that the strain with deletion of phosphofructokinase A (i.e., E. coli JW-5 ΔpfkA) produced 23.4 ± 2.1 g/L of L-tryptophan production. However, deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth, especially deletion of glucosephosphate isomerase. Next, the carbon flux in PP pathway was enhanced by introduction of… More >

MEIHUA CHEN^1,2, HUIHUI WANG³, XIAOFENG CHEN^1,2, YAN CHEN^1,2, TIANYING BIAN^1,2,*

BIOCELL, Vol.46, No.10, pp. 2281-2289, 2022, DOI:10.32604/biocell.2022.020697

Abstract Periodontitis is a frequent chronic inflammatory disorder destroying periodontium. Recent studies have revealed the role of bromodomain and extraterminal domain inhibitor (BETi) and microRNA (miR)-130a in regulating macrophage polarization and pro-inflammatory response. However, little is known about whether apabetalone (a novel BETi) and miR-130a are correlated with chronic inflammatory state in periodontitis by regulating macrophage polarization. Here murine RAW264.7 macrophages were applied as an in vitro inflammatory model. After treatment with Porphyromonas gingivalis-derived lipopolysaccharide (Pg LPS) and apabetalone, the expression of macrophage M1 polarization markers and inflammatory cytokines was assessed using real-time PCR, western blot, and enzyme-linked immuno sorbent assay… More >

HUILI ZHU¹, LINA XIAO¹, XIA YIN¹, SHIBING XIANG¹, CHUNHUI WANG^2,*

BIOCELL, Vol.46, No.10, pp. 2241-2256, 2022, DOI:10.32604/biocell.2022.020389

Abstract Pancreatic ductal adenocarcinoma (PDAC) is highly heterogeneous, making its prognosis prediction difficult. The arachidonic acid (AA) cascade is involved in carcinogenesis. Therefore, the metabolic enzymes of the AA cascade consist of lipoxygenases (LOXs), phospholipase A2s (PLA2s), and cyclooxygenases (COXs) along with their metabolic products, including leukotrienes. Nevertheless, the prognostic potential of AA metabolism-associated PDAC has not been explored. Herein, the mRNA expression patterns and the matching clinical information of individuals with PDAC were abstracted from online data resources. We employed the LASSO Cox regression model to develop a multigene clinical signature in the TCGA queue. The GEO queue and the… More >