Laura Isabel Arias-Rodríguez¹, Martha Alicia Rodríguez-Mendiola^2,*, Carlos Arias-Castro^2,*, Federico Antonio Gutiérrez Miceli¹, Diana Reséndez Pérez³, María Celina Luján Hidalgo¹, Juan José Villalobos Maldonado¹, Norma Alejandra Mancilla Margalli²

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2807-2823, 2023, DOI:10.32604/phyton.2023.030396

Abstract In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration. In our study, we specifically investigated the extract of Petroselinum crispum, a plant known for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly embryogenic cultures, and suspension… More >

WENQI CHEN^1,#, XIAOYANG CHU^2,#, YANG ZENG^3,#, YOUSHENG YAN⁴, YIPENG WANG⁴, DONGLAN SUN¹, DONGLIANG ZHANG⁵, JING ZHANG^1,*, KAI YANG^4,*

BIOCELL, Vol.47, No.7, pp. 1561-1569, 2023, DOI:10.32604/biocell.2023.028851

Abstract Background: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) has a vital role in osteogenesis. However, the mechanism underlying the regulation of ROR2 in osteogenic differentiation is still poorly comprehended. A previous study by our research group showed that a novel compound heterozygous ROR2 variation accounted for the autosomal recessive Robinow syndrome (ARRS). This study attempted to explore the impact of the ROR2: c.904C>T variant specifically on the osteogenic differentiation of BMSCs. Methods: Coimmunoprecipitation (CoIP)-western blotting was carried out to identify the interaction between ROR2 and Wnt5a. Double-immunofluorescence staining was used for determining the expressions and co-localization of ROR2 and Wnt5a… More > Graphic Abstract

JAMES L. ROSENBERG¹, WILLIAM WOOLLEY¹, IHSAN ELNUNU¹, JULIA KAMML², DAVID S. KAMMER², CLAIRE ACEVEDO^1,3,*

BIOCELL, Vol.47, No.7, pp. 1651-1659, 2023, DOI:10.32604/biocell.2023.028014

Abstract Age and diabetes have long been known to induce an oxidative reaction between glucose and collagen, leading to the accumulation of advanced glycation end-products (AGEs) cross-links in collagenous tissues. More recently, AGEs content has been related to loss of bone quality, independent of bone mass, and increased fracture risk with aging and diabetes. Loss of bone quality is mostly attributed to changes in material properties, structural organization, or cellular remodeling. Though all these factors play a role in bone fragility disease, some common recurring patterns can be found between diabetic and age-related bone fragility. The main pattern we will discuss… More >

FRANCISCO RAÚL BORZONE^1,*, MARÍA BELÉN GIORELLO¹, LEANDRO MARCELO MARTINEZ², MARÍA CECILIA SANMARTIN^1,3, LEONARDO FELDMAN⁴, FEDERICO DIMASE⁵, EMILIO BATAGELJ⁶, GUSTAVO YANNARELLI³, NORMA ALEJANDRA CHASSEING^1,*

Oncology Research, Vol.31, No.3, pp. 361-374, 2023, DOI:10.32604/or.2023.028104

Abstract Breast cancer is the predominant form of carcinoma among women worldwide, with 70% of advanced patients developing bone metastases, with a high mortality rate. In this sense, the bone marrow (BM) mesenchymal stem/stromal cells (MSCs) are critical for BM/bone homeostasis, and failures in their functionality, transform the BM into a pre-metastatic niche (PMN). We previously found that BM-MSCs from advanced breast cancer patients (BCPs, infiltrative ductal carcinoma, stage III-B) have an abnormal profile. This work aims to study some of the metabolic and molecular mechanisms underlying MSCs shift from a normal to an abnormal profile in this group of patients.… More >

LAKSHANA SADU^#, R.HARI KRISHNAN^#, R.L. AKSHAYA, I. SARANYA, UDIPT RANJAN DAS, SNEHA SATISHKUMAR, N. SELVAMURUGAN^*

BIOCELL, Vol.47, No.4, pp. 751-760, 2023, DOI:10.32604/biocell.2023.026911

Abstract MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that regulate the expression of their target mRNAs post-transcriptionally. Since their discovery, thousands of highly conserved miRNAs have been identified and investigated for their role in human health and diseases. MiR-214 has been increasingly reported to have an association with the regulation of bone metabolism. Reports suggested that miR-214 controls the critical aspects of osteoblasts (bone-forming cells), including their differentiation, proliferation, viability, and migration. Studies have also reported the functional significance of miR-214 in bone diseases and suggested its candidature as a diagnostic and therapeutic target. Further, targeting miR-214 by other ncRNAs, such… More >

Narongsak Yotha¹, Qusain Hiader², Zulqurnain Sabir³, Muhammad Asif Zahoor Raja⁴, Salem Ben Said⁵, Qasem Al-Mdallal⁵, Thongchai Botmart⁶, Wajaree Weera^6,*

CMC-Computers, Materials & Continua, Vol.74, No.2, pp. 2415-2430, 2023, DOI:10.32604/cmc.2023.031352

Abstract This study aims to solve the nonlinear fractional-order mathematical model (FOMM) by using the normal and dysregulated bone remodeling of the myeloma bone disease (MBD). For the more precise performance of the model, fractional-order derivatives have been used to solve the disease model numerically. The FOMM is preliminarily designed to focus on the critical interactions between bone resorption or osteoclasts (OC) and bone formation or osteoblasts (OB). The connections of OC and OB are represented by a nonlinear differential system based on the cellular components, which depict stable fluctuation in the usual bone case and unstable fluctuation through the MBD.… More >

Jian Peng^1,2, Yifang Zhao^1,2, Dengyong Zhang^1,2,*, Feng Li^1,2, Arun Kumar Sangaiah³

CMC-Computers, Materials & Continua, Vol.74, No.2, pp. 3405-3419, 2023, DOI:10.32604/cmc.2023.027627

Abstract Recently, object detection based on convolutional neural networks (CNNs) has developed rapidly. The backbone networks for basic feature extraction are an important component of the whole detection task. Therefore, we present a new feature extraction strategy in this paper, which name is DSAFF-Net. In this strategy, we design: 1) a sandwich attention feature fusion module (SAFF module). Its purpose is to enhance the semantic information of shallow features and resolution of deep features, which is beneficial to small object detection after feature fusion. 2) to add a new stage called D-block to alleviate the disadvantages of decreasing spatial resolution when… More >

NINGYU LI^1,2,#, XIAOFANG CHEN^2,#,§, SUXIA GENG², PEILONG LAI², LISI HUANG², MINMING LI², XIN HUANG², CHENGXIN DENG², YULIAN WANG², JIANYU WENG², XIN DU^1,2,*

BIOCELL, Vol.47, No.1, pp. 133-141, 2023, DOI:10.32604/biocell.2022.022021

Abstract The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition… More >

Syeda Naila Batool, Ghulam Gilanie^*

CMC-Computers, Materials & Continua, Vol.74, No.1, pp. 1319-1332, 2023, DOI:10.32604/cmc.2023.032121

Abstract Automated and autonomous decisions of image classification systems have essential applicability in this modern age even. Image-based decisions are commonly taken through explicit or auto-feature engineering of images. In forensic radiology, auto decisions based on images significantly affect the automation of various tasks. This study aims to assist forensic radiology in its biological profile estimation when only bones are left. A benchmarked dataset Radiology Society of North America (RSNA) has been used for research and experiments. Additionally, a locally developed dataset has also been used for research and experiments to cross-validate the results. A Convolutional Neural Network (CNN)-based model named… More >

Lilan Chen, Lu Lu, Xinlei Gong, Yichen Xu, Xiaoyuan Chu^*, Guichun Huang^*

Oncologie, Vol.24, No.3, pp. 599-604, 2022, DOI:10.32604/oncologie.2022.023310

Abstract Gastric cancer is the fifth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide in 2020. Gastric cancer usually undergoes lymph node metastasis and implantation metastasis, but bone metastasis and bone marrow invasion are rare. However, gastric cancer patients with bone marrow invasion usually have cancer emergency, so special attention should be paid in clinical practice. Herein, we analyzed the clinical characteristics of an asian gastric cancer patient with bone marrow invasion and disseminated intravascular coagulation (DIC) in our hospital and summarized the diagnosis and treatment experience to provide a reference for such diseases. More >