XIA DA¹, HAN GE², JUNFENG SHI³, CHUNHUA ZHU¹, GUOZHU WANG¹, YUAN FANG^4,*, JIN XU^1,*

Oncology Research, Vol.32, No.7, pp. 1209-1219, 2024, DOI:10.32604/or.2024.045433

Abstract Objective: This study aimed to investigate the role of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in triple-negative breast cancer (TNBC). Methods: ROR2 expression in primary TNBC and metastatic TNBC tissues was analyzed by immunohistochemical staining and PCR. ROR2 expression in TNBC cell lines was detected by PCR and Western blot analysis. The migration, invasion and chemosensitivity of TNBC cells with overexpression or knockdown of ROR2 were examined. Results: ROR2 expression was high in metastatic TNBC tissues. ROR2 knockdown suppressed the migration, invasion and chemoresistance of TNBC cells. ROR2 overexpression in MDA-MB-435 cells promoted the migration, More >

XIAOBI HUANG^1,#, CHUNYUAN CHEN², YONGYANG CHEN^1,#, HONGLIAN ZHOU¹, YONGHUA CHEN¹, ZHONG HUANG¹, YULIU XIE¹, BAIYANG LIU¹, YUDONG GUO¹, ZHIXIONG YANG¹, GUANGHUA CHEN^3,*, WENMEI SU^1,4,*

Oncology Research, Vol.32, No.7, pp. 1185-1195, 2024, DOI:10.32604/or.2023.030771

Abstract Background: Long non-coding RNAs are important regulators in cancer biology and function either as tumor suppressors or as oncogenes. Their dysregulation has been closely associated with tumorigenesis. LINC00265 is upregulated in lung adenocarcinoma and is a prognostic biomarker of this cancer. However, the mechanism underlying its function in cancer progression remains poorly understood. Methods: Here, the regulatory role of LINC00265 in lung adenocarcinoma was examined using lung cancer cell lines, clinical samples, and xenografts. Results: We found that high levels of LINC00265 expression were associated with shorter overall survival rate of patients, whereas knockdown of LINC00265 inhibited proliferation… More >

Iftikhar Naseer^1,2, Tehreem Masood^1,2, Sheeraz Akram^3,*, Zulfiqar Ali⁴, Awais Ahmad³, Shafiq Ur Rehman³, Arfan Jaffar^1,2

CMC-Computers, Materials & Continua, Vol.79, No.3, pp. 4963-4977, 2024, DOI:10.32604/cmc.2024.050204

Abstract Lung cancer is a leading cause of global mortality rates. Early detection of pulmonary tumors can significantly enhance the survival rate of patients. Recently, various Computer-Aided Diagnostic (CAD) methods have been developed to enhance the detection of pulmonary nodules with high accuracy. Nevertheless, the existing methodologies cannot obtain a high level of specificity and sensitivity. The present study introduces a novel model for Lung Cancer Segmentation and Classification (LCSC), which incorporates two improved architectures, namely the improved U-Net architecture and the improved AlexNet architecture. The LCSC model comprises two distinct stages. The first stage involves… More >

Mutasem K. Alsmadi^*

CMC-Computers, Materials & Continua, Vol.79, No.3, pp. 5175-5200, 2024, DOI:10.32604/cmc.2024.044065

Abstract Lung cancer is among the most frequent cancers in the world, with over one million deaths per year. Classification is required for lung cancer diagnosis and therapy to be effective, accurate, and reliable. Gene expression microarrays have made it possible to find genetic biomarkers for cancer diagnosis and prediction in a high-throughput manner. Machine Learning (ML) has been widely used to diagnose and classify lung cancer where the performance of ML methods is evaluated to identify the appropriate technique. Identifying and selecting the gene expression patterns can help in lung cancer diagnoses and classification. Normally,… More >

HONGBIAO WU^*, DONGFANG LIU

BIOCELL, Vol.48, No.6, pp. 971-980, 2024, DOI:10.32604/biocell.2024.049466

Abstract Background: Colorectal cancer (CRC) represents a substantial risk to public health. Bevacizumab, the first US FDA-approved antiangiogenic drug (AAD) for human CRC treatment, faces resistance in patients. The role of lipid metabolism, particularly through OPA3-regulated lipid droplet production, in overcoming this resistance is under investigation. Methods: The protein expression pattern of OPA3 in CRC primary/normal tissues was evaluated by bioinformatics analysis. OPA3-overexpressed SW-480 and HCT-116 cell lines were established, and bevacizumab resistance and OPA3 effects on cell malignancy were examined. OPA3 protein/mRNA expression and lipid droplet-related genes were measured with Western blot and qRT-PCR. OPA3… More > Graphic Abstract

JIAWEI YIN¹, YONGSHENG WANG^2,3, GUANGWEI WEI⁴, MINGXIN WEN^3,*

BIOCELL, Vol.48, No.6, pp. 959-970, 2024, DOI:10.32604/biocell.2024.049349

Abstract Objectives: This study aimed to reveal the role and possible mechanism of the ubiquitin-conjugating enzyme 2T (UBE2T) in the biological activities of breast cancer stem cells (BCSCs). Methods: The specific protein and gene expression were quantified by Western blotting and quantitative real-time polymerase chain reaction, the proportion of BCSCs was examined by flow cytometry, and the self-renewal and proliferation of BCSCs were verified by serial sphere formation and soft agar. Results: Increasing expression of UBE2T was drastically found in breast cancer than that in adjacent tissues. Furthermore, UBE2T overexpression significantly increased the proportion of BCSCs in More >

BINGJIE LI^1,#, WENBO CAO^1,2,3,#, JINJING XIAO¹, YIXIAO CHEN¹, QIYING WEI¹, MINGJIN YUE⁴, SAIJUN MO^1,2,3,*

BIOCELL, Vol.48, No.6, pp. 923-934, 2024, DOI:10.32604/biocell.2024.049160

Abstract Perineural invasion (PNI), a particularly insidious form of tumor metastasis distinct from hematogenous or lymphatic spread, has the capacity to extend well beyond the primary tumor site, infiltrating distant regions devoid of lymphatic or vascular structures. PNI often heralds a decrease in patient survival rates and is recognized as an indicator of an unfavorable prognosis across a variety of cancers. Despite its clinical significance, the underlying molecular mechanisms of PNI remain elusive, complicating the development of specific and efficacious diagnostic and therapeutic strategies. In the realm of cancer research, non-coding RNAs (ncRNAs) have attracted considerable… More >

Su-Hoon Lee, Suh-Kyung Hyun, Hak-Bong Kim, Chi-Dug Kang, Sun-Hee Kim

Oncology Research, Vol.24, No.6, pp. 495-509, 2016, DOI:10.3727/096504016X14685034103950

Abstract Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC cell lines established from primary HCC tumors, we found that CD133-high human liver cancer stem-like cells (CD133^hi) derived from the SNU-475 cell line were highly susceptible to TRAIL compared to other HCC cell lines with a small population of CD133. CD133^hi SNU-475 cells showed upregulation of TRAIL… More >

Jian Zhang^*1, ZhenFeng Shi^†1, JinXing Huang^‡, XiaoGuang Zou^§

Oncology Research, Vol.24, No.6, pp. 487-494, 2016, DOI:10.3727/096504016X14685034103752

Abstract This study aimed to investigate the pivotal role of cystatin B (CSTB) in the development of gastric cancer and to explore its possible regulatory mechanism. Human gastric cancer SGC-7901 cells as a model in vitro were transfected with plasmid PCDNA3.1-CSTB and siRNA-CSTB using Lipofectamine 2000. Quantitative realtime PCR (qRT-PCR) and Western blotting were performed to determine the relative expression of CSTB and PI3K/Akt/mTOR pathway-related protein. Moreover, MTT assay, Transwell assay, and flow cytometry were used to assess cell proliferation, migration, and apoptosis, respectively. The results showed that CSTB was significantly downregulated in SGC-7901 cells compared… More >

Yi Miao¹, Meng Lu¹, Qin Yan, Shuangdi Li, Youji Feng

Oncology Research, Vol.24, No.6, pp. 463-475, 2016, DOI:10.3727/096504016X14685034103671

Abstract Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNAPKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell migration, and More >