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  • Open Access

    ARTICLE

    Silicon and Nitric Oxide-Mediated Regulation of Growth Attributes, Metabolites and Antioxidant Defense System of Radish (Raphanus sativus L.) under Arsenic Stress

    Savita Bhardwaj1, Tunisha Verma1, Ali Raza2,*, Dhriti Kapoor1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 763-782, 2023, DOI:10.32604/phyton.2023.025672

    Abstract

    Arsenic (As) contaminated food chains have emerged as a serious public concern for humans and animals and are known to affect the cultivation of edible crops throughout the world. Therefore, the present study was designed to investigate the individual as well as the combined effects of exogenous silicon (Si) and sodium nitroprusside (SNP), a nitric oxide (NO) donor, on plant growth, metabolites, and antioxidant defense systems of radish (Raphanus sativus L.) plants under three different concentrations of As stress, i.e., 0.3, 0.5, and 0.7 mM in a pot experiment. The results showed that As stress reduced the growth parameters of… More >

  • Open Access

    ARTICLE

    Salt Stress Affects the Growth and Yield of Wheat (Triticum aestivum L.) by Altering the Antioxidant Machinery and Expression of Hormones and Stress-Specific Genes

    Shahid Hussain1, Rui Zhang1, Shuli Liu1, Yang Wang1, Irshad Ahmad2, Yinglong Chen1, Hongyan Hou3, Qigen Dai1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 861-881, 2023, DOI:10.32604/phyton.2023.025487

    Abstract

    Understanding physiological responses in saline agriculture may facilitate wheat breeding programs. Based on a screening test, the Ningmai-14 (NM-14) and Yangmai-23 (YM-23) wheat cultivars were selected for further experiments to understand the underlying salinity tolerance mechanism. This study investigated the effects of five salinity levels such as Control (CK) = 0 (without NaCl stress), S1 = 0.20%, S2 = 0.25%, S3 = 0.30% and S4 = 0.35% of NaCl concentrations of soil on wheat plants. The results showed that increased salinity concentration reduced the growth and yield of wheat cultivars (NM-14 and YM-23). However, YM-23 (12.7%) yielded more than NM-14… More >

  • Open Access

    ARTICLE

    Biochemical and Physiological Responses of Arabidopsis thaliana Leaves to Moderate Mechanical Stimulation

    Iva Šutevski1,#, Klara Krmpotić1,#, Sandra Vitko1, Nataša Bauer1, Eva Fancev2, Mario Cifrek2, Željka Vidaković-Cifrek1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 901-920, 2023, DOI:10.32604/phyton.2023.025165

    Abstract Mechanical stimulation of plants can be caused by various abiotic and biotic environmental factors. Apart from the negative consequences, it can also cause positive changes, such as acclimatization of plants to stress conditions. Therefore, it is necessary to study the physiological and biochemical mechanisms underlying the response of plants to mechanical stimulation. Our aim was to evaluate the response of model plant Arabidopsis thaliana to a moderate force of 5 N (newton) for 20 s, which could be compared with the pressure caused by animal movement and weather conditions such as heavy rain. Mechanically stimulated leaves were sampled 1 h… More >

  • Open Access

    ARTICLE

    The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

    XIAOXU ZHANG1,2, LIN ZHANG1, LIN DU3, HUIYAN SUN4, XIA ZHAO2, YANG SUN1, WEI WANG2,*, LISHENG WANG1,3,*

    BIOCELL, Vol.47, No.2, pp. 385-392, 2023, DOI:10.32604/biocell.2023.025203

    Abstract Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. However, during in vitro expansion, MSCs are prone to aging, which largely limits their application. Prostaglandin E-2 (PGE-2) is a key effector secreted by MSCs to exert immunomodulatory effects. By screening the compound library for PGE-2 secretion, the antioxidant trolox was verified as a stimulator of MSCs to secrete PGE-2. The effect of antioxidant trolox on biological characteristics of MSCS, including aging, proliferation, and gene expression, was examined. The results demonstrated that trolox can resist aging,… More >

  • Open Access

    ARTICLE

    Inhibition of H2O2-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROS-mediated ERK/p53 signaling pathways

    MIAOMIAO LI1,#, DANYANG CHEN2,#, JUNYI KE1, RUILIN ZHENG2, JINGYAO SU2, ZILIN ZHENG1, JIEYI LUO1, HANRAN MAI1, FAN JIANG1, YANXIA QU1, XIAOQIONG GU1, BING ZHU2, YINGHUA LI2,*, LIANDONG ZUO1,*

    BIOCELL, Vol.47, No.2, pp. 401-408, 2023, DOI:10.32604/biocell.2023.025154

    Abstract A H2O2-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nano-selenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of selenium-containing antioxidant enzymes induced by oxidative stress. A safe and effective nano-drug system of functionalized selenium-containing nanoparticles (SeNPs) was developed with lentinan (LNT) (SeNPs@LNT). Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h to evaluate the cytotoxicity of selenium. GC2-spg cells were randomly divided into the following groups: control, hydrogen peroxide (H2O2), SeNPs@LNT, and H2O2+SeNPs@LNT groups. H2O2+SeNPs@LNT group was… More >

  • Open Access

    ARTICLE

    Growth, ROS Markers, Antioxidant Enzymes, Osmotic Regulators and Metabolic Changes in Tartary Buckwheat Subjected to Short Drought

    Yan Wan1,#, Yuan Liang1,#, Xuxiao Gong1,#, Jianyong Ouyang1, Jingwei Huang2, Xiaoyong Wu1, Qi Wu1, Changying Liu1, Xueling Ye1, Xiaoning Cao3, Gang Zhao1, Liang Zou1,*, Dabing Xiang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 35-54, 2023, DOI:10.32604/phyton.2022.021698

    Abstract Tartary buckwheat (Fagopyrum tataricum) is an important pseudocereal feed crop with medicinal and nutritional value. Drought is one of the main causes of reduced growth and yield in these plants. We investigated the growth, physiological, and metabolic responses of the widely promoted Tartary buckwheat variety Chuan Qiao No. 1 to polyethylene glycol (PEG)-mediated drought stress. Drought significantly decreased shoot length, shoot biomass and relative water content. Root length, malondialdehyde content, electrolyte leakage, activities of superoxide dismutase, peroxidase, catalase and amylase, and contents of soluble sugar, soluble protein and proline were increased by PEG-mediated drought. Untargeted metabolomics analysis identified 32 core… More >

  • Open Access

    ARTICLE

    Extraction, Antioxidant Activity and Identification of Flavonoids from Root Tubers of Kosteletzkya virginica

    Yu Zai*, Shaohua Liu

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 225-236, 2023, DOI:10.32604/phyton.2022.022576

    Abstract Kosteletzkya virginica (K. virginica) is used for revegetation of salt-affected coastal tidal flats and as a raw material of biodiesel. K. virginica root tuber, a biowaste with low economic value, is rich in bioactive compounds. This study aimed to extract and identify flavonoids from K. virginica root tubers. The optimal extraction conditions were 1/25 (w/v) solid/liquid ratio, 40% ethanol concentration at 40°C for 60 min. Under these conditions, 65.2 ± 3.7 mg/g total flavonoid content was extracted from the roots, which were collected from salinized soil in late autumn of the third year. Antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl, hydroxyl… More >

  • Open Access

    ARTICLE

    Effects of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria on growth and reactive oxygen metabolism of tomato fruits under low saline conditions

    WEI ZHOU, MENGMENG ZHANG, KEZHANG TAO, XIANCAN ZHU*

    BIOCELL, Vol.46, No.12, pp. 2575-2582, 2022, DOI:10.32604/biocell.2022.021910

    Abstract Land salinization is a major form of land degradation, which is not conducive to the growth and quality of fruits and vegetables. Plant salt tolerance can be enhanced by arbuscular mycorrhizal fungi (AMF) or plant growth-promoting rhizobacteria (PGPR). This study examined the effects of inoculation with PGPR singly or in combination with AMF, on the growth and quality of tomato fruits under low saline conditions. Tomatoes were cultivated in a greenhouse with sterilized soil, inoculated with PGPR, AMF, or co-inoculated with PGPR and AMF, and NaCl solution (1%) was added to the soil. The results indicated that AMF + PGPR… More >

  • Open Access

    ARTICLE

    Synthesis and Antioxidant Activity of (E) ω-Formylcamphene-Based Thiazole Hydrazone Derivatives

    Jiayu Chang1, Qingying Ding1, Chong Zhong1, Yao Long2, Zhuanquan Xiao3, Hai Luo1, Yuling Yang1, Zongde Wang1, Shengliang Liao1, Jinzhu Chen2, Shangxing Chen1,*, Ji Zhang1,*

    Journal of Renewable Materials, Vol.11, No.1, pp. 379-391, 2023, DOI:10.32604/jrm.2023.022535

    Abstract (E) ω-formylcamphene was synthesized from α-pinene, the main component of turpentine, and then reacted with thiosemicarbazide to obtain (E) ω-formylcamphene thiosemicarbazide 3, which was reacted with 14 α-bromoacetophenone compounds to obtain 14 (E) ω-formylcamphene thiazole hydrazone compounds 5a5n; the yields were all above 80%. The structures of the target compounds were characterized by IR, 1H-NMR, 13C-NMR, and HR-MS analyses. Then, 500, 250, 125, 62.5, and 31.25 mg/L drug solutions were prepared. Free radical scavenging experiments of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) were carried out with Trolox and L-ascorbic acid as the control samples. The scavenging… More >

  • Open Access

    ARTICLE

    Application of two forms of silicon and their impact on the postharvest and the content of bioactive compounds in cucumber (Cucumis sativus L.) fruits

    YOLANDA GONZÁLEZ-GARCÍA1, VALERIA FLORES-ROBLES2, GREGORIO CADENAS-PLIEGO3, ADALBERTO BENAVIDES-MENDOZA4, MARCELINO CABRERA DE LA FUENTE4, ALBERTO SANDOVAL-RANGEL4, ANTONIO JUÁREZ-MALDONADO1,*

    BIOCELL, Vol.46, No.11, pp. 2497-2506, 2022, DOI:10.32604/biocell.2022.021861

    Abstract The metabolic activity of the fruits continues even after harvest, which results in the loss of bioactive compounds, a decrease in the quality of the fruits, softening and browning, among other negative effects. The use of certain elements such as silicon can improve postharvest quality, since it is involved in the metabolic, physiological and structural activity of plants, moreover can increase the quality of the fruits. In addition, nanotechnology has had a positive impact on crop yield, nutritional value, fruit quality and can improve antioxidant activity. For these reasons, the use of beneficial elements such as silicon in the form… More >

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