Rosa Della Monica^*1, Mariella Cuomo^*†1, Roberta Visconti^‡, Annabella di Mauro^§, Michela Buonaiuto^*, Davide Costabile^*†, Giulia De Riso^†, Teodolinda Di Risi^*, Elia Guadagno^¶, Roberto Tafuto^#, Sabrina Lamia^**, Alessandro Ottaiano^††, Paolo Cappabianca^#, Maria Laura Del Basso de Caro^¶, Fabiana Tatangelo^§, Juergen Hench^‡‡, Stephan Frank^‡‡, Salvatore Tafuto^**, Lorenzo Chiariotti^*†

Oncology Research, Vol.28, No.9, pp. 837-845, 2020, DOI:10.3727/096504021X16214197880808

Abstract Unresectable neuroendocrine neoplasms (NENs) often poorly respond to standard therapeutic approaches. Alkylating agents, in particular temozolomide, commonly used to treat high-grade brain tumors including glioblastomas, have recently been tested in advanced or metastatic NENs, where they showed promising response rates. In glioblastomas, prediction of response to temozolomide is based on the assessment of the methylation status of the MGMT gene, as its product, O6 -methylguanine-DNA methyltransferase, may counteract the damaging effects of the alkylating agent. However, in NENs, such a biomarker has not been validated yet. Thus, we have investigated MGMT methylation in 42 NENs of different grades and from… More >

FEIFEI TIAN¹, HUAN HU¹, DI WANG¹, HUAN DING¹, QINGJIA CHI², HUAPING LIANG³, WENLI ZENG^4,*

BIOCELL, Vol.46, No.10, pp. 2291-2301, 2022, DOI:10.32604/biocell.2022.020198

Abstract This study used transcriptome and epigenetic data to predict the prognosis of immune-related genes (IRGs) Apelin (APLN) in patients with hepatocellular carcinoma (HCC). The TCGA database has gene expression and clinical data for HCC. And DNA methylation 450 k data for HCC was download from the University of California Santa Cruz (UCSC) Xena browser. Performing clinical and prognostic analysis of APLN expression, results show that APLN is highly expressed in tumor samples. And it has an increasing trend with the development of clinical stage and T stage. To explore the prognostic role of APLN, the Immune-related DNA methylation (DNAm) sites… More >

Yanqing Wu^1,2, Jiao Liu¹, Yuhan Tang², Daqiu Zhao², Jun Tao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 2005-2016, 2022, DOI:10.32604/phyton.2022.021062

Abstract The ornamental and commercial values of herbaceous peony (Paeonia lactiflora Pall.) are directly related to its flower pattern. However, the molecular mechanisms underlying the type formation of P. lactiflora flowers have not been studied in great detail. Previous studies identified, using integrated multipleomics analysis, revealed that APETALA2 (AP2) is an important candidate gene that modulates type formation of P. lactiflora flowers. To further reveal the expression mechanism of AP2 in P. lactiflora petals, we examined the profile of AP2 expression in the inner and outer petals of ‘ZiFengyu’ at various developmental stages using qRT-PCR and BSP+Miseq methylation analysis. Based on… More >

Xingyu Zhu^1,2,#, Yongjun Wang^3,#, Shuang Chang^4,#, Yue Su², Cuixia He¹, Shuang Hu⁵, Minhui Zhu¹, Yuzhou Ding², Nuannuan Ren², Qizhi Wang², Jing Xie^1,*, Huan Zhou^1,2,*

Oncologie, Vol.23, No.4, pp. 569-588, 2021, DOI:10.32604/oncologie.2021.018869

Abstract Sirtuins (SIRTs), members of the enzyme family found in yeast cells, are related to silent information regulator (SIR) 2 homologous to its gene family. SIRTs play an important role in many physiological functions from overexpression of gene silencing at the molecular level to the expression of related proteins and RNA to apoptosis. Studies have indicated that SIRTs may be related to the occurrence, development, and metastasis of cancer. However, the current mechanism of action of SIRTs in various diseases and the principle of molecular biology are not fully understood. Therefore, the present article discusses the main regulatory role and function… More >

KAVITHA GOVARTHANAN^1,*, PIYUSH KUMAR GUPTA², BINITA ZIPPORAHE¹, REKHA GAHTORI³, SOUMYA PANDIT², RAM PRASAD^4,*

BIOCELL, Vol.45, No.3, pp. 501-515, 2021, DOI:10.32604/biocell.2021.014441

Abstract Stem cells constitute the source of cells that replenishes the worn out or damaged cells in our tissue and enable the tissue to carry out the destined function. Tissue-specific stem cells are compartmentalized in a niche, which keeps the stem cells under quiescent condition. Thus, understanding the molecular events driving the successful differentiation of stem cells into several lineages is essential for its better manipulation of human applications. Given the developmental aspects of the cell, the cellular function is greatly dependent on the epigenomics signature that in turn governs the expression profile of the cell. The stable inheritance of the… More >

MENGDAN WU¹, MENGYAO SUN¹, QINHUAI LAI¹, YIN LU¹, YUYIN FU¹, YUJIA PENG¹, WEIRONG LAI¹, LISHI ZENG¹, SHENGYAN ZHAO¹, YUYAN LI¹, ZHIXIONG ZHANG¹, XIAOFENG CHEN¹, FAN QIAO¹, YIWEN ZHANG^1,*, SHIJIE ZHOU^1,2,*, LANTU GOU¹, JINLIANG YANG^1,2

BIOCELL, Vol.45, No.3, pp. 589-597, 2021, DOI:10.32604/biocell.2021.013882

Abstract The chemokine ligand 13-chemokine receptor 5 (CXCL13-CXCR5) axis has been characterized as a critical tumor-promoting signaling pathway in the tumor microenvironment (TME) in multiple types of solid tumors. In this study, we analyzed the expression profile of CXCL13 in kidney clear cell carcinoma (KIRC) and its correlation with tumor-infiltrating immune cells (TIICs). A monoclonal antibody against CXCL13 with high affinity and purity was generated in our lab for western blot and immunohistochemistry (IHC). Bioinformatic analysis was performed based on bulk-seq data from the Cancer Genome Atlas (TCGA)-KIRC and single-cell RNA-seq data from scRNASeqDB and PanglaoDB. Results showed that high CXCL13… More >

Heyam H. Al-Baity^*, Nourah Al-Mutlaq

CMC-Computers, Materials & Continua, Vol.67, No.3, pp. 3089-3106, 2021, DOI:10.32604/cmc.2021.015291

Abstract Machine-learning algorithms have been widely used in breast cancer diagnosis to help pathologists and physicians in the decision-making process. However, the high dimensionality of genetic data makes the classification process a challenging task. In this paper, we propose a new optimized wrapper gene selection method that is based on a nature-inspired algorithm (simulated annealing (SA)), which will help select the most informative genes for breast cancer prediction. These optimal genes will then be used to train the classifier to improve its accuracy and efficiency. Three supervised machine-learning algorithms, namely, the support vector machine, the decision tree, and the random forest… More >

Yan Song^1,2, Zhixin Wang³, Xin Zhang⁴, Rong Zhang¹, Jinchun Li^1,2,*

Journal of Renewable Materials, Vol.9, No.5, pp. 943-957, 2021, DOI:10.32604/jrm.2021.014131

Abstract Lignin is a natural biopolymer with a complex three-dimensional network, commercially obtained from waste liquid of paper pulp and bioethanol production, and could be a candidate for preparation of environment-friendly bio-based polyphenol material. In the present work, the demethylated wheat straw alkali lignin (D-Lig), prepared by demethylation of wheat straw alkali lignin (Lig) using an in-situ generated Lewis acid, was used to synthesize bio-based phenol formaldehyde resin adhesive (D-LPF) applied in plywood. Effects of synthetic process’s factors, including lignin substitution for phenol, NaOH concentration and molar ratio of formaldehyde to phenol, on the bonding strength and free formaldehyde content of… More >

YONG LIU^1,2,#, ZHIHUA JU^1,#, QIANG JIANG¹, WENHAO LIU¹, CHUNHONG YANG¹, YARAN ZHANG¹, XIUGE WANG¹, YAPING GAO¹, XIAOCHAO WEI¹, YAN SUN¹, JINPENG WANG¹, MINGHAI HOU¹, LING YANG², JINMING HUANG^1,*

BIOCELL, Vol.45, No.1, pp. 79-87, 2021, DOI:10.32604/biocell.2021.013583

Abstract F-box and WD-40 domain protein 11 (FBXW11) is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis. In our previous research, the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility. In the present study, the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups. The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules. Three splice variants of… More >

Andrew D. Spearman

Congenital Heart Disease, Vol.12, No.6, pp. 828-833, 2017, DOI:10.1111/chd.12543

Abstract A genetic basis of congenital heart disease (CHD) has been known for decades. In addition to the sequence of the genome, the contribution of epigenetics to pediatric cardiology is increasingly recognized. Multiple epigenetic mechanisms, including DNA methylation, histone modification, and RNA-based regulation, are known mediators of cardiovascular disease, including both development and progression of CHD and its sequelae. Basic understanding of the concepts of epigenetics will be essential to all pediatric cardiologists in order to understand mechanisms of pathophysiology, pharmacotherapeutic concepts, and to understand the role of epigenetics in precision medicine. More >