Genhai Hu^1,*, Maoni Chao¹, Xiuren Zhou², Yuanzhi Fu²

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2535-2554, 2023, DOI:10.32604/phyton.2023.030163

Abstract Gossypium hirsutum L. is an important cash crop native to the subtropics and is widely cultivated around the world. Low temperature is an important stress that seriously affects seed germination and emergence during planting. In this study, transcriptomic profiles of low-temperature- and normal-temperature-germinated seeds of Xinluzao 25, a variety with low-temperature tolerance and high germination rates, were analyzed and compared. The following results were obtained. (1) A total of 81.06 Gb of clean data were obtained after transcriptome sequencing and assembly, and 76,931 non-redundant Unigene sequences were obtained after data consolidation and concatenation; of these, 69,883 Unigene sequences were annotated.… More >

Xiaolan Guo^1,2, Jinbin Hu³, Shimei Yang⁴, Delu Wang^2,*, Jianbing Wang¹

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820

Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family… More >

José Adrian Perez-Robles, Carlos Alberto Lecona-Guzmán^*, Víctor Manuel Ruíz-Valdiviezo, Joaquín Adolfo Montes-Molina^*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2503-2518, 2023, DOI:10.32604/phyton.2023.029784

Abstract The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L. infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts. Solanum lycopersicum plantlets were transplanted 15 days after the emergency. Five days after transplanting, Beauveria bassiana spores were applied at a concentration of 1 × 10⁷ spores mL⁻¹ onto soil (along with A. indica (N) and P. auritum (H) leaf extracts) where S. lycopersicum plants were planted. Eight days after transplanting, spores of F. oxysporum strain were applied at a concentration of 1 × 10⁶ spores mL⁻¹ to… More >

Tongman Zhao^1,2,#, Yujie Fan^1,2,#, Yabing Cao^1,2, Xiaogai Zhao^1,2, Guoqiang Fan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2473-2488, 2023, DOI:10.32604/phyton.2023.029469

Abstract Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. We identified 12 SERK genes with relatively conserved gene structures and motifs that were distributed unevenly on eight Paulownia chromosomes. The gene promoters contained various cis-acting elements that regulated the expression of the PfSERK genes in response to hormones and abiotic stresses. Synteny analysis indicated that 10 segmental duplication events had occurred during evolution of the PfSERK family. The expression… More >

Chen Su, Jie Hong, Jiang Wang, Yang Yang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2611-2632, 2023, DOI:10.32604/phyton.2023.029457

Abstract The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing, field crop management and yield estimation. Calculating the number of seedlings is inefficient and cumbersome in the traditional method. In this study, a method was proposed for efficient detection and calculation of rapeseed seedling number based on improved you only look once version 5 (YOLOv5) to identify objects and deep-sort to perform object tracking for rapeseed seedling video. Coordinated attention (CA) mechanism was added to the trunk of the improved YOLOv5s, which made the model more effective in identifying shaded,… More >

Xiao Han, Songnan Yang, Xueying Li, Qiulin Wu, Yongyi Xing, Jun Zhang^*, Fenglou Ling^*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2577-2589, 2023, DOI:10.32604/phyton.2023.029440

Abstract Peanut, with high oil content, has been a major oil and food crop globally. The compositions of the fatty acids are the common factors in determining the oil quality. In the present study, an F2 segregated population with 140 individuals derived from the cross of Weihua8 (a cultivar) and 12L49 (a line with high oleic acid concentration) was used to construct a genetic map and conduct QTL mapping analysis. A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci. Within the 103 SSR loci, a genetic linkage map, covering a total… More >

Jie Zhang¹, Yingwei Zhao², Hongying Li³, Jianwei Ni^4,*, Dongmei Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2591-2610, 2023, DOI:10.32604/phyton.2023.028338

Abstract Paper mulberry is a high-quality woody feed resource plant with high crude protein content. It is widely distributed in China and has excellent characteristics of salt and alkali tolerance. Paper mulberry has ecological and economic importance. Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth. In the saline-alkali environments, transcriptome expression is altered leading to phenotypic defects in most plants. However, the regulatory mechanism related to paper mulberry’s salt-stress (SS) response is not clearly understood. In the present study de novo transcriptomic assembly was performed, and gene expression levels were measured… More >

Sang-Mo Kang^1,#, Shifa Shaffique^1,#, Muhammad Imran^2,#, Su-Mi Jeon³, Shabir Hussain Wani⁵, Muhammad Aaqil Khan⁴, Peter Odongkara¹, Eun-Hae Kwon¹, Yosep Kang¹, Joon-Ik Son⁶, Won-Chan Kim^1,*, In-Jung Lee^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2699-2711, 2023, DOI:10.32604/phyton.2023.028224

Abstract Food waste is recognized as a valuable source for potential agricultural applications to supply organic matter and nutrients to arable soil. However, the information on the combined application of food waste and the plant growth-promoting bacterial strain, Chlorella, related to plant metabolic features and sodium chloride content in arable soil is limited. The present study was conducted to investigate the exogenous application of food waste along with Chlorella, which improved the physio-morphological features of red pepper. Our results revealed that this combination enhanced the organic matter in the soil, ultimately improving the fertility rate of the soil, and the physio-morphological… More >

Tahira Kalsoom¹, Touqeer Ahmed¹, Muhammad Azam Khan¹, Mirza Hasanuzzaman^2,*, Mukhtar Ahmed^3,*, Stefaan P. O. Werbrouck⁴

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2429-2450, 2023, DOI:10.32604/phyton.2023.030846

Abstract Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro propagation. Plantlets were raised from True Potato Seeds (TPS) in test tubes containing 10 mL of Murashige Skoog (MS) medium. Subculturing was done after every 21 days and after four subsequent subculturing, when there was an adequate quantity of stock material, uninodal cuttings of about 3–4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1–2 mm on each side of the node. Prepared uninodal cuttings were cultured on… More >

Sibel Day^*, Nilüfer Koçak-Şahin

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2461-2471, 2023, DOI:10.32604/phyton.2023.029920

Abstract Aromatic and medicinal plant species having small seeds have field emergence problems due to low nutrient supply. Therefore, Pimpinella anisum seeds were hydro and osmoprimed with 100 mM MgCl₂, CaCl₂, and ZnCl₂, for 2, 4, and 8 h each to compare their growth attributes during germination and seedling establishment stages. Nontreated seeds were used as control. Both hydro and osmo primed seeds were dried for 48 h before, they were sown in plastic trays in growth room conditions to see the impact of treatments on seedling emergence and growth. The maximum root length (12.90 cm), fresh weight (256.30 mg plant⁻¹),… More >