NINGYU LI^1,2,#, XIAOFANG CHEN^2,#,§, SUXIA GENG², PEILONG LAI², LISI HUANG², MINMING LI², XIN HUANG², CHENGXIN DENG², YULIAN WANG², JIANYU WENG², XIN DU^1,2,*

BIOCELL, Vol.47, No.1, pp. 133-141, 2023, DOI:10.32604/biocell.2022.022021 - 26 September 2022

Abstract The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation More >

MONALISA MISHRA^1,2,*, PUNYATOYA PANDA¹, BEDANTA KUMAR BARIK¹, AMRITA MONDAL¹, MRUTUNJAYA PANDA¹

BIOCELL, Vol.47, No.1, pp. 51-69, 2023, DOI:10.32604/biocell.2022.023392 - 26 September 2022

Abstract Exposure to some toxic compounds causes structural and behavioral anomalies associated with the neurons in the later stage of life. Those toxic compounds are termed as a neurotoxicant, which can be a physical factor, a toxin, an infection, radiation, or maybe a drug. The incongruities caused due to a neurotoxicant further depend on the toxicity of the compound. More importantly, the neurotoxicity of the compound is associated with the concentration and the time point of exposure. The neurodevelopmental defect appears depending on the toxicity of the compound. A neurodevelopmental defect may be associated with a More >

YUEHUA SHI^1,#, QIUYING YAN^2,#, QIN LI³, WEI QIAN¹, DONGYAN QIAO¹, DONGDONG SUN², HONG YU^1,*

BIOCELL, Vol.47, No.1, pp. 165-173, 2023, DOI:10.32604/biocell.2022.023043 - 26 September 2022

Abstract The placenta plays an important role in nutrient transport to maintain the growth and development of the embryo. Gestational diabetes mellitus (GDM), the most common complication during pregnancy, highly affects placental function in late gestation. Advanced glycation end-products (AGEs), a complex and heterogeneous group of compounds engaged by the receptor for AGEs (RAGE), are closely associated with diabetes-related complications. In this study, AGEs induced a decrease in the expression of tight junction (TJ) proteins in BeWo cells and increased the paracellular permeability of trophoblast cells by regulating RAGE/NF-κB. Sprague-Dawley (SD) rats injected with 100 mg/kg… More >

YINGHUI GAO, DENGTAI WEN^*, SHIJIE WANG, JINGFENG WANG

BIOCELL, Vol.47, No.1, pp. 41-49, 2023, DOI:10.32604/biocell.2022.022689 - 26 September 2022

Abstract Presenilin (Psn) protein is associated with organismal aging. Mutations in the Psn gene may lead to Alzheimer’s disease (AD), dilated cardiomyopathy (DCM), and many age-dependent degenerative diseases. These diseases seriously affect the quality of life and longevity of the population and place a huge burden on health care and economic systems around the world. Humans have two types of Psn, presenilin-1 (PSEN1) and presenilin-2 (PSEN2). Mutations in the genes encoding PSEN1, PSEN2, and amyloid precursor protein (APP) have been identified as the major genetic causes of AD. Psn is a complex gene strongly influenced by genetic and… More >

MINGZHAO LI¹, QIAN ZHANG², WENBIN HUANG¹, SHIYING ZHANG¹, NAN JIANG², XIAOSHUAI HUANG^3,*, FENG CHEN^2,*

BIOCELL, Vol.47, No.1, pp. 205-214, 2023, DOI:10.32604/biocell.2022.022572 - 26 September 2022

Abstract Cancer progression involves the sonic hedgehog (SHH) pathway, in which the receptor PTCH1 actives the downstream pathways. Dysfunction of PTCH1 can lead to nevoid basal cell carcinoma Syndrome (NBCCs) including neoplastic disease and congenital disorder. To evaluate the relationship between PTCH1 and cancer, we applied the CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells (GMSM-K). Then we screened six PTCH1 variants associated with cleft lip/palate (CL/P), one of the congenital disorders in NBCCs, and generated PTCH1 variant and wild-type recombinant PTCH1^−/− GMSM-K cell lines. Transcriptome sequencing was conducted in these cell lines. The… More >

JINXIAN LIU¹, JINGFANG FENG², CHANG ZHANG², YONGJUAN REN², WEIHUA SU², GUANGHENG WU¹, XIANYU FU¹, NING HUANG³, YOUXIONG QUE², HUI LING^3,*, JUN LUO^2,*

BIOCELL, Vol.47, No.1, pp. 215-225, 2023, DOI:10.32604/biocell.2022.022477 - 26 September 2022

Abstract Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study, the effects of a sugarcane ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in transgenic Arabidopsis thaliana and Escherichia coli cells were evaluated. The results demonstrated a significant negative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A. thaliana, as indicated by the phenotypes. In addition, the expression of AtP5CS and AtRD29A, two genes tightly related to stress resistance, was significantly lower in the overexpression lines than in the wild type. The growth More >

BINFEN HOU¹, LI ZHAO¹, T IANHAO ZHAO¹, MINGMING YANG¹, WANWAN ZHU¹, XIAODONG CHEN², XIQUAN KE¹, ZHENZENG MA¹, LIN GU¹, MENG WANG¹, MIN DENG^1,*

BIOCELL, Vol.47, No.1, pp. 175-186, 2023, DOI:10.32604/biocell.2022.021359 - 26 September 2022

Abstract Aim: Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported to possess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigate the effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells. Methods: Human GC cell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation, migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwell assay, and flow cytometry. Cell migration and invasion were reassessed in… More >

Lisheng Luo^1,*, Xinran Xie¹, Yongqiang Zhang¹, Xiaofeng Zhang², Xinyue Cui¹

Journal of Renewable Materials, Vol.11, No.2, pp. 791-809, 2023, DOI:10.32604/jrm.2022.022539 - 22 September 2022

Abstract Traditional methods focus on the ultimate bending moment of glulam beams and the fracture failure of materials with defects, which usually depends on empirical parameters. There is no systematic theoretical method to predict the stiffness and shear distribution of glulam beams in elastic-plastic stage, and consequently, the failure of such glulam beams cannot be predicted effectively. To address these issues, an analytical method considering material nonlinearity was proposed for glulam beams, and the calculating equations of deflection and shear stress distribution for different failure modes were established. The proposed method was verified by experiments and More > Graphic Abstract

Yunxia Zhou¹, Hisham Essawy², Ai Liu¹, Chenyu Yang¹, Defa Hou¹, Xiaojian Zhou^1,*, Guanben Du¹, Jun Zhang^1,*

Journal of Renewable Materials, Vol.11, No.2, pp. 837-852, 2023, DOI:10.32604/jrm.2022.022792 - 22 September 2022

Abstract This study presents an easily prepared film based on alkaline starch-polyvinyl alcohol hybrid and lignin fiber as an additive (SPL film). The SPL film was prepared under acidic conditions through a polycondensation reaction of PVA and a mixture incorporating alkaline starch and lignin fiber from agriculture or forest source. The examination using scanning electron microscopy (SEM) showed that the surface of SPL film was smooth and the lignin fiber had good compatibility within the film hybrid. Electrospray ionization mass spectroscopy (ESI-MS) and fourier transform infrared (FTIR) investigations indicated that alkaline starch and lignin fiber reacted More > Graphic Abstract

Lixian Wu^1,#, Yongquan Li^1,#, Qiaoguang Li², Jingxuan Li¹, Qinghua Lao¹, Yucheng Zhong¹, Hui Zhang^1,*, Xiu Hu^1,*

Journal of Renewable Materials, Vol.11, No.2, pp. 975-989, 2023, DOI:10.32604/jrm.2022.023547 - 22 September 2022

Abstract This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family. The inhibitory rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of leaf and stem, and root and rhizome extracts from Alpinia katsumadai Hayata, Alpinia oxyphylla Miq × Alpinia henryi K. Schumann, Alpinia oblongifolia Hayata, Alpinia nigra (Gaertn.) Burtt, Amomum villosum Lour, Alpinia zerumbet (Pers.) Burtt. et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method. The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi. At a 1.5 mg mL⁻¹, A. zerumbet… More >