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  • Open Access

    ARTICLE

    Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance

    Siyan Liu1,*, Jinfeng Liu1, Yuzhe Zhang1, Yushi Jiang1, Shaowang Hu1, Andi Shi1, Qiyao Cong1, Shuyan Guan2,*, Jing Qu2, Yao Dan1

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1465-1482, 2022, DOI:10.32604/phyton.2022.018836 - 14 March 2022

    Abstract Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were… More >

  • Open Access

    ARTICLE

    Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry (HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines

    Simiao Wang1,#, Xiaohang Li1,#, Meiting Jiang1, Xinlong Wu2, Yuying Zhao1, Meiyu Liu1, Xiaoyan Xu1, Huimin Wang1, Hongda Wang1, Heshui Yu2, Wenzhi Yang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1353-1364, 2022, DOI:10.32604/phyton.2022.021093 - 14 March 2022

    Abstract Quality control of ginseng currently is mainly based on ginsenoside analysis, but rarely focuses on the volatile organic components. In the current work, an untargeted metabolomics approach, by headspace solid-phase micro-extraction gas chromatography/mass spectrometry (HS-SPME-GC/MS), was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines, which included P. ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), red ginseng (PGR), P. ginseng leaf (PGL), P. quinquefolius leaf (PQL), P. notoginseng leaf (PNL), P. ginseng flower (PGF), P. quinquefolius flower (PQF), P. notoginseng flower (PNF), P. japonicus (PJ), and P. japonicus var. major (PJvm). Chromatographic separation was performed… More >

  • Open Access

    REVIEW

    Sorghum: Nutritional Factors, Bioactive Compounds, Pharmaceutical and Application in Food Systems: A Review

    Heba I. Mohamed1,*, Eman M. Fawzi1, Abdul Basit2, Kaleemullah3, Rafiq Lone4, Mahmoud R. Sofy5

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1303-1325, 2022, DOI:10.32604/phyton.2022.020642 - 14 March 2022

    Abstract After wheat, rice, maize, and barley, sorghum is the fifth most widely grown cereal on the planet. Due to its high production, drought resistance, and heat tolerance, this crop is replacing maize in some areas. Sorghum is available in a variety of colors, including cream, lemon-yellow, red, and even black. The principal grain anatomical components are pericarp, germ or embryo and endosperm. This review provides an overview of key sorghum grain components, including starches, fiber, proteins, lipids, and vitamins. Also, we summarized phenolic compounds, flavonoids, tannins, carotenoids, vitamin E, amines, Policosanols and Phytosterols in sorghum… More >

  • Open Access

    ARTICLE

    Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola (Brassica napus L.)

    Reza Shokri-Gharelo1, Ali Bandehagh1,*, Mohammad Anwar Hossain2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1403-1417, 2022, DOI:10.32604/phyton.2022.020431 - 14 March 2022

    Abstract Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic… More >

  • Open Access

    ARTICLE

    Seasonal Changes in Soil Respiration with An Elevation Gradient in Abies nephrolepis (Trautv.) Maxim. Forests in North China

    Zhijie Tian1,*, Xueying Jia2, Tingting Liu1, Eryan Ma1, Lamei Xue1, Yanqiu Hu1, Qingrong Zheng1

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1543-1556, 2022, DOI:10.32604/phyton.2022.020329 - 14 March 2022

    Abstract Soil respiration (Rs) plays an important role in regulating carbon cycle of terrestrial ecosystems and presents temporal and spatial heterogeneity. Abies nephrolepis is a tree species that prefers the cold and wet environment and is mainly distributed in Northeast Asia and East Asia. The Rs variations of Abies nephrolepis forests communities are generally environmental-sensitive and can effectively reflect the adaptive responses of forest ecosystems to climate change. In this study, the growing-seasonal variations of Rs, soil temperature, soil water content and soil properties of Abies nephrolepis forests were analyzed along an altitude gradient (2000, 2100, 2200 and 2300… More >

  • Open Access

    ARTICLE

    Proteomics Analysis of Soybean Seedlings under Short-Term Water Deficit

    Xiyue Wang, Zihao Wu, Chao Yan, Chunmei Ma, Shoukun Dong*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1381-1401, 2022, DOI:10.32604/phyton.2022.020251 - 14 March 2022

    Abstract Soybeans are one of the most important grain crops worldwide. Water deficit, which seriously affects the yield and quality of soybeans, is the main abiotic stress factor in soybean production. As a follow-up study, the droughttolerant soybean variant Heinong 44 was analyzed via proteome analysis. Soybean was exposed to water deficit for 0, 8, and 24 h, and protein samples were extracted for detection of differentially expressed proteins. Protein sequencing of leaf tissues under water stress yielded a total of 549 differentially expressed proteins: 75 and 320 upregulated proteins as well as 70 and 84 More >

  • Open Access

    ARTICLE

    Effects of Different Irrigation Management on the Textural Properties of Double-Cropping Late Indica Rice in South China

    Ruoyu Xiong1, Liming Chen1, Daren Jiang1, Yingjie Zhou1, Xueming Tan1, Xiaohua Pan1, Yongjun Zeng1, Jun Zhang2, Yanhua Zeng1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1495-1502, 2022, DOI:10.32604/phyton.2022.018485 - 14 March 2022

    Abstract In order to investigate the effects of different irrigation management on the textural properties of two different double-cropping late Indica rice, the effects of three irrigation management including conventional irrigation (CK), constant irrigation (CI) and alternate wetting and drying (AWD) on textural properties has been researched under field conditions of two years. The results indicated that the firmness, cohesiveness and chewiness were decreased under AWD treatment, and the stickiness was increased compared with CK, while the textural properties under CI treatment showed the opposite trend with AWD treatment. Additionally, AWD treatment signifi- cantly improved the springiness More >

  • Open Access

    ARTICLE

    Effects of High Temperature and Strong Light Combine Stress on Yield and Quality of Early Indica Rice with Different Amylose Content during Grout Filling

    Xiaofeng Ai1, Ruoyu Xiong1, Xueming Tan1, Haixia Wang1, Jun Zhang2, Yongjun Zeng1, Xiaohua Pan1, Qinghua Shi1, Taoju Liu1, Yanhua Zeng1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1257-1267, 2022, DOI:10.32604/phyton.2022.018328 - 14 February 2022

    Abstract High temperature (HT) accompanied with strong light (SL) often occurs in early indica rice production during grout filling stage in southern China, which accelerates grain ripening. Two indica rice cultivars with different amylose content (Zhongjiazao17, ZJZ17, high amylose content; Xiangzaoxian45, XZX45, low amylose content) were grown under control (CK), HT, and HT+SL conditions during grout filling to determine the effects on grain yield and quality of rice. The results showed that compared with CK, HT and HT+SL significantly reduced the 1000-grain weight and filled grain rate whether in high or low amylose content early indica rice cultivars… More >

  • Open Access

    ARTICLE

    Phenotypic Characterization of Oryza nivara (Sharma et Shastry) Collected from Different Ecological Niches of Sri Lanka

    Salinda Sandamal1,2,3,#, Asanka Tennakoon1,#, Parakkrama Wijerathna1, Song Ge2,3, DABN Amarasekera4, Buddhi Marambe5, Sara M. Elwany6, Sobhy Sorour6, Ayman El Sabagh6, Mohamed M. Hassan7, Disna Ratnasekera1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1269-1287, 2022, DOI:10.32604/phyton.2022.018983 - 14 February 2022

    Abstract Information on the genetic diversity of wild rice species in Sri Lanka is relatively meagre, though it plays a key role in crop improvement programs of cultivated rice (Oryza sativa L.). The present study was carried out to identify the morphological variation pattern of the wild populations of O. nivara in Sri Lanka. Seven populations (P1 to P7) collected from different agro-ecological regions were characterized in a common garden based on nine morphological traits. The findings revealed a high level of phenotypic variation between populations when compared to within a population. The most variable traits were the… More >

  • Open Access

    REVIEW

    Opportunities and Challenges of Plant Bioactive Compounds for Food and Agricultural-Related Areas

    Maicon S. N. dos Santos1, João H. C. Wancura2, Carolina E. D. Oro3, Rogério M. Dallago3, Marcus V. Tres1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1105-1127, 2022, DOI:10.32604/phyton.2022.020913 - 14 February 2022

    Abstract The growing development of biological products highlights the social and environmental responsibility that several industrial companies are facing in recent years. In this context, the advancement of bioprocessing as an alternative for exploring the potential of ecologically based products, especially in biofuels, food, and agro-industrial business, exposes the rational efficiency of the application of renewable sources in different industrial segments. Industries strongly associated with food production concentrate large amounts of wastes rich in bioactive compounds. A range of highly effective technologies has been highly explored to recover large concentrations of prominent compounds present in these… More >

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