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  • Open Access

    ARTICLE

    Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

    MIZUNA YANO1, YUTA UMEHARA1, TOMOKAZU KUDO1, TAKAO NAKAMURA2, TADASHI KOSAWADA1, ATSUYOSHI NISHINA3, MASAKI SAZUKA4, DAISUKE SATO1,*, ZHONGGANG FENG1,*

    BIOCELL, Vol.45, No.5, pp. 1213-1229, 2021, DOI:10.32604/biocell.2021.016281 - 12 July 2021

    Abstract While fatty acids play essential roles in the physiology of the myocardium, conventional culture media contain little lipid. We previously revealed that rat neonatal myocardium mainly contains docosahexaenoic (DHA), linoleic (LA), and arachidonic (AA) acids as polyunsaturated fatty acids (PUFAs), and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal myocardium. In this study, we first assessed the effects of supplementation of DHA, LA, or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat cardiomyocytes. Based on this assessment,… More >

  • Open Access

    ARTICLE

    Potential Antidiabetic and Anti-Genotoxic Activities of Silver Nanoparticles of Alkaloid Extract of Rhazya stricta in Rat Animal Model

    Mohammed Ali Ashehri*

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1749-1762, 2021, DOI:10.32604/phyton.2021.016290 - 28 June 2021

    Abstract In most of Arabian courtiers Rhazya stricta is extensively utilized in public medicine for several diseases treatment. In this study, crude alkaloid extract of R. stricta (CAERS) coated with silver nanoparticles (CAERS-AgNPs) as potential treatment against diabetes in DM animal model was evaluated. Swiss albino male rats (n = 80) were injected with STZ to induce Diabetes Mellitus type-2 (DM). DM-rats were injected different doses of CAERS or CAERS-nanoparticles (CAERS-NPs) for 2 months. The results exhibited that mRNA expression of insulin and insulin receptor was down-regulated, activity levels of antioxidant enzymes were decreased, generation of ROS mediated DNA adducts… More >

  • Open Access

    ARTICLE

    Phenotype, Physiology, and Gene Expression of Barley Seedlings in Response to Nano Zinc Oxide Stress

    Mengyuan Dong#, Rong Sun#, Qianhui Yang, Lantian Zhang, Yangying Yong, Yunxia Fang, Xian Zhang, Xiaoqin Zhang*, Dawei Xue*

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1589-1598, 2021, DOI:10.32604/phyton.2021.017465 - 28 June 2021

    Abstract In recent years, zinc oxide nanoparticles (ZnO NPs) have been widely used as zinc fertilizers and pesticides. The use of ZnO NPs in this way can provide benefits to humans, but also has potential risks. ZnO NPs inevitably enter the environment during their production and use, which affects the ecological environment and crop growth. In order to investigate the phenotype, physiology, and gene expression of barley (Hordeum vulgare L.) seedlings under ZnO NPs stress, the barely cultivars ZJU3 (P21), Golden Promise (GP) and L23 were chosen for study. Different ZnO NPs concentrations were applied to compare… More >

  • Open Access

    ARTICLE

    An Evolutionary Algorithm for Non-Destructive Reverse Engineering of Integrated Circuits

    Huan Zhang1,2, Jiliu Zhou1,2,*, Xi Wu2

    CMES-Computer Modeling in Engineering & Sciences, Vol.127, No.3, pp. 1151-1175, 2021, DOI:10.32604/cmes.2021.015462 - 24 May 2021

    Abstract In hardware Trojan detection technology, destructive reverse engineering can restore an original integrated circuit with the highest accuracy. However, this method has a much higher overhead in terms of time, effort, and cost than bypass detection. This study proposes an algorithm, called mixed-feature gene expression programming, which applies non-destructive reverse engineering to the chip with bypass detection data. It aims to recover the original integrated circuit hardware, or else reveal the unknown circuit design in the chip. More >

  • Open Access

    ARTICLE

    Effects of Different Vermicompost Rates on Growth, 2-Acetyl-1-Pyrroline, Photosynthesis and Antioxidant Responses of Fragrant Rice (Oryza sativa L.) Seedling

    Shaoyi Ruan1,2,3,#, Haowen Luo1,2,3,#, Xiaoyan Zeng1,2,3,#, Runhao Wen1,2,3, Feida Wu1,2,3, Xiangru Tang1,2.*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1273-1283, 2021, DOI:10.32604/phyton.2021.015610 - 27 April 2021

    Abstract Vermicompost is an organic fertilizer contains multiple nutrient elements. However, the application of vermicompost in fragrant rice production is rarely reported. In order to study the effects of vermicompost application on fragrant rice (Oryza sativa L.) seedling performances, present study was conducted with two fragrant rice cultivars and four vermicompost rate treatments (0 (CK), 2.5 (Wo1), 5.0 (Wo2) and 10.0 (Wo3) g kg−1 ). The results showed that vermicompost treatments significantly increased dry weight of fragrant rice seedling by 8.31–32.56% compared with CK. 21.10–59.13% higher net photosynthetic rates and 10.66–59.16% higher chlorophyll contents (chlorophyll a, chlorophyll b More >

  • Open Access

    ARTICLE

    Exploration and Validation of the Potential Downstream Genes Underlying ipa1-2D Locus for Rice Panicle Branching

    Lin Zhang1,2,*, Dong Xie1, Zhong Bian1, Yiting Zou1, Han Zhou1, Wenlu Cai1, Hadi Yeilaghi1, Xiaolei Fan1,2, Changquan Zhang1,2, Qiaoquan Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 773-787, 2021, DOI:10.32604/phyton.2021.015229 - 30 March 2021

    Abstract In recent years, some super hybrid rice varieties were bred with strong culms and large panicles, which are mainly contributed by the ipa1-2D locus. A gain-of-function allele of OsSPL14 is the ipa1-2D and it can greatly increase the panicle primary branch number. However, the key downstream genes mediating this trait variation are not fully explored. In this study, we developed high-quality near-isogenic lines (NILs) with a difference of only 30 kb chromosomal segment covering the ipa1-2D locus. Using the NILs, we explored the impact of ipa1-2D on five sequential stages of early inflorescence development, and found that the… More >

  • Open Access

    ARTICLE

    Differential Responses of NHX1 and SOS1 Gene Expressions to Salinity in two Miscanthus sinensis Anderss. Accessions with Different Salt Tolerance

    Qian Sun1,2, Toshihiko Yamada3, Yulai Han2,*, Tetsuo Takano1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 827-836, 2021, DOI:10.32604/phyton.2021.013805 - 30 March 2021

    Abstract The lignocellulosic crop Miscanthus spp. has been identified as a good candidate for biomass production. The responses of Miscanthus sinensis Anderss. to salinity were studied to satisfy the needs for high yields in marginal areas and to avoid competition with food production. The results indicated that the relative advantages of the tolerant accession over the sensitive one under saline conditions were associated with restricted Na+ accumulation in shoots. Seedlings of two accessions (salt-tolerant ‘JM0119’ and salt-sensitive ‘JM0099’) were subjected to 0 (control), 100, 200, and 300 mM NaCl stress to better understand the salt-induced biochemical responses of… More >

  • Open Access

    ARTICLE

    Genome-wide Analysis of a Plant AT-rich Sequence and Zinc-binding Protein (PLATZ) in Triticum Aestivum

    Xiaohang He1, Minjie Liu2, Zhengwu Fang1, Dongfang Ma1,2,3,*, Yilin Zhou1,3,*, Junliang Yin1

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 971-986, 2021, DOI:10.32604/phyton.2021.012726 - 30 March 2021

    Abstract Plant AT-rich sequence and zinc-binding protein (PLATZ) is a plant transcription factor that has been studied in corn. PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression. It is involved in the regulation of dehydration tolerance in seeds. In this study, we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes. We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice, Arabidopsis, and maize. Phylogenetic analysis on PLATZ gene sequences from different species was performed. We found that PLATZs could be divided into three groups.… More >

  • Open Access

    ARTICLE

    Soil Moisture Prediction in Peri-urban Beijing, China: Gene Expression Programming Algorithm

    Hongfei Niu1,2, Fanyu Meng3, Huanfang Yue3, Lihong Yang4, Jing Dong2,5, Xin Zhang2,5,*

    Intelligent Automation & Soft Computing, Vol.28, No.1, pp. 93-106, 2021, DOI:10.32604/iasc.2021.010131 - 17 March 2021

    Abstract Soil moisture is an important indicator for agricultural planting and agricultural water management. People have been trying to guide crop cultivation, formulate irrigation systems, and develop intelligent agriculture by knowing exactly what the soil moisture is in real time. This paper considers the impact of meteorological parameters on soil-moisture change and proposes a soil-moisture prediction method based on the Gene Expression Programming (GEP) algorithm. The prediction model is tested on datasets from Shunyi, Yanqing and Daxing agricultural farms, Beijing. The results show that the GEP model can predict soil moisture with a maximum correlation coefficient More >

  • Open Access

    ARTICLE

    Transcriptome analysis of purple pigment formation in Colocasia esculenta

    FANGLIAN HE1, WEIQING DONG1,*, SHAOLONG WEI2,*, ZUYANG QIU3, JINGLI HUANG4, HUIPING JIANG1, SHIYU HUANG1, LILI LIU3

    BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418 - 03 March 2021

    Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing.… More >

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