Iqra Ishaaq¹, Muhammad Umer Farooq^1,2,3,4,*, Syeda Anjum Tahira², Rizwana Maqbool¹, Celaleddin Barutçular³, Muhammad Yasir⁵, Saira Bano², Zaid Ulhassan⁶, Ghassan Zahid⁷, Muhammad Ahsan Asghar⁸, Sajad Hussain⁹, Kocsy Gabor⁸, Ulkar Ibrahimova^10,11, Jianqing Zhu⁴, Anshu Rastogi^12,*

Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2733-2758, 2022, DOI:10.32604/phyton.2022.022094

Abstract Drought is the major detrimental environmental factor for wheat (Triticum aestivum L.) production. The exploration of genetic patterns underlying drought tolerance is of great significance. Here we report the gene actions controlling the phenological traits using the line × tester model studying 27 crosses and 12 parents under normal irrigation and drought conditions. The results interpreted via multiple analysis (mean performance, correlations, principal component, genetic analysis, heterotic and heterobeltiotic potential) disclosed highly significant differences among germplasm. The phenological waxiness traits (glume, boom, and sheath) were strongly interlinked. Flag leaf area exhibits a positive association with peduncle… More >

Qiang Ai, Cuijing Liu^*, Mei Han^*, Limin Yang

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2537-2548, 2022, DOI:10.32604/phyton.2022.021889

Abstract Iris domestica is a plant of the Iridaceae family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of I. domestica by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of I. domestica to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in I. domestica under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that EF1β was More >

Shuang Song¹, Xiaomei Li², Xin Wang¹, Qi Zhou¹, Yongping Li¹, Xiaojing Wang¹, Shoukun Dong^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2475-2490, 2022, DOI:10.32604/phyton.2022.021090

Abstract Soybean is an important legume food crop, and its seeds are rich in nutrients, providing humans and animals with edible oil and protein feed. However, soybean is sensitive to water requirements, and drought is an important factor limiting soybean yield and quality. This study used Heinong 84 (drought resistant variety) and Hefeng 46 (intermediate variety) as tested varieties planted in chernozem, albic, and black soils. The effects of drought stress on the activities of key enzymes in carbon metabolism and photosynthetic characteristics of soybean were studied during the flowering stage, most sensitive to water. (1)… More >

Taufika Islam Anee^1,#, Md. Nur Nabi Islam^1,#, Mohamed M. Hassan², Abdul Awal Chowdhury Masud¹, Md. Mahabub Alam¹, Mirza Hasanuzzaman^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1959-1972, 2022, DOI:10.32604/phyton.2022.021137

Abstract Due to the unpredictable climate change, drought stress is being considered as one of the major threats to crop production. Wheat (Triticum aestivum L. cv. BARI Gom-26) being a dry season crop frequently faces scarcity of water and results in a lower yield. Therefore, this experiment aims to explore the role of different organic amendments (OAs) in mitigating drought stress-induced damage. The pot experiment consisted of different organic amendments viz. compost, vermicompost and poultry manure @0.09 kg m⁻² soil, biochar @2.5% w/w soil and chitosan @1% w/w soil which was imposed on the plants grown under both… More >

MEIGE WANG, JUAN GONG, CHUNHUI SONG, ZHENGYANG WANG, SHANGWEI SONG, JIAN JIAO, MIAOMIAO WANG, XIANBO ZHANG^*, TUANHUI BAI^*

BIOCELL, Vol.46, No.7, pp. 1763-1770, 2022, DOI:10.32604/biocell.2022.018934

Abstract

Drought stress is one of the major environmental obstacles that limit the production and development of apples (Malus domestica Borkh.). The role of melatonin is well known in the protection of plants under environmental stresses. In this study, we investigated the effect of melatonin on apple rootstock M. hupehensis Rehd under drought stress. The results showed that drought inhibited the growth of M. hupehensis and dramatically reduced root surface area, root volume, the number of tips and forks, and root diameter. Drought-induced growth inhibition was significantly decreased by adding melatonin. Net photosynthetic rate (Pn), intercellular CO₂ concentration (Ci), stomatal

… More >

Siyan Liu^1,*, Jinfeng Liu¹, Yuzhe Zhang¹, Yushi Jiang¹, Shaowang Hu¹, Andi Shi¹, Qiyao Cong¹, Shuyan Guan^2,*, Jing Qu², Yao Dan¹

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1465-1482, 2022, DOI:10.32604/phyton.2022.018836

Abstract Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were… More >

Reza Shokri-Gharelo¹, Ali Bandehagh^1,*, Mohammad Anwar Hossain^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1403-1417, 2022, DOI:10.32604/phyton.2022.020431

Abstract Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic… More >

Yuzhe Zhang^1,#, Guanglong Li^1,#, Shaowang Hu¹, Jinfeng Liu¹, Yushi Jiang¹, Siyan Liu^2,*, Shuyan Guan^2,*, Jing Qu², Dan Yao¹, Andi Shi¹, Yixuan Liu¹

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1183-1198, 2022, DOI:10.32604/phyton.2022.018853

Abstract Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance… More >

Yuting Luan¹, Cong Xu¹, Xiaoxiao Wang¹, Daqiu Zhao¹, Jun Tao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1045-1059, 2022, DOI:10.32604/phyton.2022.018767

Abstract The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed More >

FENGJUN DAI¹, ZIYI RONG¹, QIANGSHENG WU¹, ELSAYED FATHI ABD_ALLAH³, CHUNYAN LIU^1,2,*, SHENGRUI LIU^2,*

BIOCELL, Vol.46, No.5, pp. 1339-1346, 2022, DOI:10.32604/biocell.2022.018909

Abstract Tea plants are sensitive to soil moisture deficit, with the level of soil water being a critical factor affecting their growth and quality. Arbuscular mycorrhizal fungi (AMF) can improve water and nutrient absorption, but it is not clear whether AMF can improve the photosynthetic characteristics of tea plants. A potted study was conducted to determine the effects of Claroideoglomus etunicatum on plant growth, leaf water status, pigment content, gas exchange, and chlorophyll fluorescence parameters in Camellia sinensis cv. Fuding Dabaicha under well-watered (WW) and drought stress (DS) conditions. Root mycorrhizal colonization and soil hyphal length were significantly… More >