GANG LIU^1,#, LEI SHI^1,#, BIN WANG¹, ZEHUI WU¹, HAIYUAN ZHAO¹, TIANYU ZHAO², LIANGHUI SHI^1,*

Oncology Research, Vol.32, No.3, pp. 585-596, 2024, DOI:10.32604/or.2023.029349

Abstract The role of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colon cancer involves various tumorigenic processes and has been studied widely. However, the mechanism by which it promotes colon cancer remains unclear. Retroviral vector pSEB61 was retrofitted in established HCT116-siKCN and SW480-siKCN cells to silence KCNQ1OT1. Cellular proliferation was measured using CCK8 assay, and flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) analysis showed differentially expressed genes (DEGs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.… More >

XIANGDONG SUN^1,2,#, HUIJUAN WEN^1,2,#, FAZHAN LI^1,2, IHTISHAM BUKHARI^1,2, FEIFEI REN^1,2, XIA XUE^1,2, PENGYUAN ZHENG^1,2,*, YANG MI^1,2,*

Oncology Research, Vol.32, No.2, pp. 283-296, 2024, DOI:10.32604/or.2023.044618

Abstract Nicotinamide adenine dinucleotide (NAD+) plays an essential role in cellular metabolism, mitochondrial homeostasis, inflammation, and senescence. However, the role of NAD+-regulated genes, including coding and long non-coding genes in cancer development is poorly understood. We constructed a prediction model based on the expression level of NAD+ metabolism-related genes (NMRGs). Furthermore, we validated the expression of NMRGs in gastric cancer (GC) tissues and cell lines; additionally, β-nicotinamide mononucleotide (NMN), a precursor of NAD+, was used to treat the GC cell lines to analyze its effects on the expression level of NMRGs lncRNAs and cellular proliferation, cell… More > Graphic Abstract

JIWEI SUN^1,2,#, LIANG XU^1,#, YESEN ZHANG², HAORAN LI¹, JIE FENG², XUEFENG LU², JUN DONG^1,*

BIOCELL, Vol.47, No.12, pp. 2721-2733, 2023, DOI:10.32604/biocell.2023.043869

Abstract Objective: This investigation aimed to unveil new prospective diagnosis-related biomarkers together with treatment targets against glioblastoma. Methods: The expression levels of long non-coding RNA (lncRNA) DPP10-AS1 were assessed using real-time quantitative polymerase chain reaction (RT-qPCR) within both the patient tissue specimens and glioblastoma cell lines. The relationship between lncRNA DPP10-AS1 expression in glioblastoma and patient prognosis was investigated. Cell Counting Kit-8 (CCK-8), transwell, and clonogenic experiments were utilized to assess tumor cells’ proliferation, invasiveness, and migratory potentials after lncRNA DPP10-AS1 expression was up or down-regulated. Using an online bioinformatics prediction tool, the intracellular localization of lncRNA… More >

CONGCHEN BAI¹, QIHANG KONG², HAO TANG², SHUWEN ZHANG², JUNTENG ZHOU^3,*, XIAOJING LIU^2,4,*

BIOCELL, Vol.47, No.11, pp. 2517-2533, 2023, DOI:10.32604/biocell.2023.043864

Abstract Background: Various physiological mechanisms are linked to dilated cardiomyopathy (DCM) development, including oxidative stress, immune irregularities, inflammation, fibrosis, and genetic changes. However, precise molecular drivers of DCM, especially regarding abnormal immune responses, remain unclear. This study investigates immune-related long non-coding RNAs (lncRNAs) in DCM’s diagnostic and therapeutic potential. Methods: GSE141910, GSE135055, and GSE165303 datasets were acquired from the GEO database. LASSO, SVM-RFE, and random forest algorithms identified DCM-associated immune-related lncRNAs. Diagnostic capabilities were assessed by Nomogram and receiver operating characteristic (ROC) curves. Multivariate linear regression explored lncRNA correlations with ejection fraction. Single-sample gene set enrichment analysis… More > Graphic Abstract

ZHAOXU YAO^*, HAIBIN MA, LIN LIU, QIAN ZHAO, LONGCHAO QIN, XUEYAN REN, CHUANJUN WU, KAILI SUN

BIOCELL, Vol.47, No.9, pp. 1965-1975, 2023, DOI:10.32604/biocell.2023.030796

Abstract Objective: Through integrated bioinformatics analysis, the goal of this work was to find new, characterised N7-methylguanosine modification-related long non-coding RNAs (m7G-lncRNAs) that might be used to predict the prognosis of laryngeal squamous cell carcinoma (LSCC). Methods: The clinical data and LSCC gene expression data for the current investigation were initially retrieved from the TCGA database & sanitised. Then, using co-expression analysis of m7G-associated mRNAs & lncRNAs & differential expression analysis (DEA) among LSCC & normal sample categories, we discovered lncRNAs that were connected to m7G. The prognosis prediction model was built for the training category… More >

LU SUN^1,2, HUAICHENG TAN¹, TING YU³, RUICHAO LIANG^4,*

Oncology Research, Vol.31, No.6, pp. 967-988, 2023, DOI:10.32604/or.2023.042309

Abstract Lung adenocarcinoma (LUAD) is the most common and deadliest subtype of lung cancer. To select more targeted and effective treatments for individuals, further advances in classifying LUAD are urgently needed. The number, type, and function of T cells in the tumor microenvironment (TME) determine the progression and treatment response of LUAD. Long noncoding RNAs (lncRNAs), may regulate T cell differentiation, development, and activation. Thus, our aim was to identify T cell-related lncRNAs (T cell-Lncs) in LUAD and to investigate whether T cell-Lncs could serve as potential stratifiers and therapeutic targets. Seven T cell-Lncs were identified… More >

WUYAO LIU, CHANGBAO QU, XIAOLU WANG^*

Oncology Research, Vol.31, No.4, pp. 543-567, 2023, DOI:10.32604/or.2023.029563

Abstract The high immune infiltration and heterogeneity of the microenvironment in clear cell renal cell carcinoma (ccRCC) result in the variability of prognosis and clinical response. While PANoptosis has strong immunogenicity and is worthy of further study. In this study, data from The Cancer Genome Atlas database was used to obtain immune-related PANoptosis lncRNAs with prognostic value. Subsequently, the role of these lncRNAs in cancer immunity, progression and the therapeutic response was analyzed, and a new prediction model was constructed. Additionally, we further explored the biological value of PANoptosis-related lncRNAs using single-cell data from the Gene… More >

LI TIAN^1,#, YONGYI HUANG^1,#, BAOZHEN ZHANG^2,#, YI SONG^1,#, LIN YANG³, QIANQIAN CHEN¹, ZHENG WANG³, YILING WANG¹, QIHAN HE¹, WENHAN YANG¹, SHUYONG YU⁴, TIANYU LU⁵, ZICHEN LIU¹, KAIPING GAO^1,*, XIUJUN FAN^2,*, JIAN SONG^4,*, RIHONG ZHAI^1,*

Oncology Research, Vol.31, No.4, pp. 463-479, 2023, DOI:10.32604/or.2023.028791

Abstract Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge. By RNA-sequencing analysis, we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA. LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion. Silencing of LLNLR-299G3.1 with ASO (antisense oligonucleotide) resulted in opposite effects. Mechanistically, LLNLR-299G3.1 bound to cancer-associated RNA binding proteins and regulated the expression… More >

CHUYAO WANG^1,2,#, CHUAN LU^2,#, LUXIANG ZOU^2,*, DONGMEI HE^2,*

BIOCELL, Vol.47, No.6, pp. 1293-1306, 2023, DOI:10.32604/biocell.2023.028199

Abstract Background: Temporomandibular joint osteoarthritis (TMJOA) is an end-stage disease that seriously affects the patients’ quality of life. Molecular insights in advancing our understanding of TMJOA are the need of the hour. Methods: We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed (DE) long non-coding RNA (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) in human synovial TMJOA tissues. Firstly, synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs, miRNAs, and mRNAs. We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression, and… More >

YANG CAO^1,#, YINING LIU^1,#, YANG YU¹, XIAOFEI GUO¹, XIUXIU WANG¹, WENYA MA¹, HANJING LI², ZHONGYU REN², XINLU GAO², SIJIA LI², HAOYU JI², HONGYANG CHEN², HONG YAN², YANAN TIAN², XIN WANG², BENZHI CAI^1,2,*

BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080

Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression… More >