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ARTICLE

piR-37524 Overexpression in Colorectal Cancer: A Potential Diagnostic Bio-Marker and Therapeutic Target

Jiaxi Li#, Deepak Iyer#, Siming Sui, Zheng Huang, Ryan Wai-Yan Sin, Abraham Tak-Ka Man, Wai-Lun Law, Chi-Chung Foo*, Lui Ng*
Department of Surgery, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, 999077, China
* Corresponding Author: Chi-Chung Foo. Email: email; Lui Ng. Email: email
# Jiaxi Li and Deepak Iye contributed equally to this study and share first authorship
(This article belongs to the Special Issue: Tumor Biomarkers for Diagnosis, Prognosis and Targeted Therapy)

Oncology Research https://doi.org/10.32604/or.2026.074981

Received 22 October 2025; Accepted 31 December 2025; Published online 23 January 2026

Abstract

Objectives: Piwi-associated RNAs are small non-coding RNAs implicated in cancer, yet few have been characterized in colorectal cancer (CRC). This study aimed to identify a CRC-related piRNA and investigate its clinical relevance, biological function, and biomarker potential. Methods: Candidates were identified by reanalysis of small-RNA sequencing. piR-37524 was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) in colorectal cancer tissues, matched adjacent non-tumor tissues, colorectal adenomas, liver metastases, and serum samples from patients and healthy controls. Clinicopathological correlations and diagnostic performance were evaluated. Functional assays included 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation, colony formation, and wound-healing migration in HCT116 and HT29 cells after piR-37524 inhibition. RNA sequencing and Western blotting examined epithelial–mesenchymal transition (EMT) markers and nuclear factor-kappa B (NF-κB) components. Results: piR-37524 was significantly overexpressed in CRC compared with adjacent non-tumor tissues and was associated with larger tumor size, poorer differentiation, and distant metastasis. Elevated expression was also observed in colorectal adenomas and liver metastases. Serum piR-37524 levels were increased in patients with adenomas and CRC compared with healthy controls, indicating diagnostic potential. Functional assays demonstrated that piR-37524 inhibition suppressed CRC cell proliferation and migration, accompanied by changes consistent with epithelial–mesenchymal transition regulation. Mechanistic analyses implicated tumor necrosis factor alpha-induced protein 3 (TNFAIP3)-associated NF-κB signaling. Conclusions: piR-37524 is an oncogenic piRNA in CRC that promotes progression via the TNFAIP3/NF-κB/EMT axis, serving as a potential pan-stage diagnostic biomarker and therapeutic target.

Keywords

Colorectal cancer; piRNAs; tumor biomarker

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