Open Access
ARTICLE
Upregulation of Sox2 Following Saracatinib Treatment Contributes to a Resistant Phenotype in Colorectal Cancer Cells under Growth Factor-Supplemented Conditions
Chanwoong Yoon1,#, Euihyeon Na2,#, Min Joo Choi1, Sang-Pil Yoon1,3,*
1 Interdisciplinary Postgraduate Program in Biomedical Engineering, Graduate School, Jeju National University, Jeju, Republic of Korea
2 Department of Psychiatry, Soonchunhyang University Cheonan Hospital, Soonchunhyang University College of Medicine, Cheonan, Republic of Korea
3 Department of Anatomy, College of Medicine, Jeju National University, Jeju, Republic of Korea
* Corresponding Author: Sang-Pil Yoon. Email: 
# These authors contributed equally to this work
Oncology Research https://doi.org/10.32604/or.2026.074140
Received 03 October 2025; Accepted 29 January 2026; Published online 21 February 2026
Abstract
Objective: Increased Src kinase activity is known to correlate with cancer progression and poor prognosis, indicating that Src plays a central role in cell migration and invasion. In this study, we investigated the effects of saracatinib, a Src kinase inhibitor, under anoikis-resistant conditions in colorectal cancer cells. Methods: Wild-type and 5-fluorouracil-resistance acquired SNU-C5 colorectal cancer cells were cultured in both monolayer and spheroid systems under fetal bovine serum (FBS) or growth factor (GF) supplemented conditions. Cell viability assay, flow cytometry, wound healing assay, spheroid formation and morphometric analysis, and Western blotting were performed using both adherent cells and spheroids. Results: Saracatinib significantly reduced cell viability and migration in both cell lines, predominantly through the induction of apoptosis. Spheroid formation was less efficient under GF-supplemented conditions than under FBS-supplemented conditions. The anti-cancer effects of saracatinib were mediated through inhibition of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated protein kinase (ERK), or epidermal growth factor receptor (EGFR) signaling pathways. Although most cancer stem cell (CSC) markers were suppressed by saracatinib, expression of sex determining region Y-box-2 (Sox2) was paradoxically increased in monolayer cultures. Upon re-treatment with saracatinib, Sox2-upregulated cells formed larger spheroids under GF-supplemented conditions compared with wild-type cells. Conclusions: Saracatinib exerts anti-cancer effects in colorectal cancer cells by downregulating MAPKs, EGFR, and CSC-associated markers. However, paradoxical upregulation of Sox2 influenced spheroid formation under GF-supplemented conditions, suggesting that Sox2 may contribute to drug resistance or recurrence in colorectal cancers.
Keywords
Src inhibitor; drug resistance; epithelial-mesenchymal transition; sex determining region Y-box-2; colorectal cancer
Login
Register
Submit a Paper
Propose a Special lssue
Download PDF
Downloads
Citation Tools
