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ARTICLE

Anargyros Kouris¹, Aikaterini Pistiki², Alexandros Katoulis¹, Marianna Georgitsi², Sofia Giatrakou¹, Evangelia Papadavid¹, Mihai G. Netea³, Nikolaos Stavrianeas¹, Evangelos J. Giamarellos-Bourboulis^2,3
European Cytokine Network, Vol.25, No.4, pp. 63-68, 2014, DOI:10.1684/ecn.2014.0358
Abstract Background: Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level. Objective: To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in More >

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Open Access

ARTICLE

Alton G. Sutter¹, Arun P. Palanisamy¹, Justin D. Ellet¹, Michael G. Schmidt², Rick G. Schnellmann^3,4, Kenneth D. Chavin¹
European Cytokine Network, Vol.25, No.4, pp. 69-76, 2014, DOI:10.1684/ecn.2015.0359
Abstract Steatotic livers are more sensitive to ischemia/reperfusion (I/R) and are thus routinely rejected for transplantation because of their increased rate of primary nonfunction (PNF). Lean livers have less I/R-induced damage and inflammation due to Kupffer cells (KC), which are protective after total, warm, hepatic I/R with associated bowel congestion. This protection has been linked to KC-dependent expression of the potent anti-inflammatory cytokine interleukin-10 (IL-10).We hypothesized that pretreatment with exogenous IL-10 would protect the steatotic livers of genetically obese (ob/ob) mice from inflammation and injury induced by I/R. Lean and ob/ob mice were pretreated with either More >

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