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Harika Atmaca¹, Selim Uzunoglu²
European Cytokine Network, Vol.25, No.1, pp. 1-7, 2014, DOI:10.1684/ecn.2014.0347
Abstract Trabectedin, a tetrahydroisoquinoline alkaloid derived from a Caribbean tunicate Ecteinascidia turbinata, has been shown to have antitumor effects. In this study, we assessed the possible anti-angiogenic effects of trabectedin on human umbilical vein endothelial cells (HUVECs) and breast cancer cell lines. An XTT cell viability assay was used to determine cytotoxicity. A scratch assay was used to detect the migration of cells after trabectedin treatment. Angiogenic cytokine profiles of breast cancer cell lines, before and after treatment with trabectedin, were investigated using an angiogenesis antibody array. Changes in mRNA expression levels of VEGF were evaluated… More >

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ARTICLE

Martin Kováč^1,2, Martina Vášková^1,2, Denisa Petráčková³, Vendula Pelková^1,2, Ester Mejstříková^1,2, Tomáš Kalina^1,2, Markéta Žaliová^1,2, Jaroslav Weiser³, Jan Starý², Ondřej Hrušák^1,2
European Cytokine Network, Vol.25, No.1, pp. 8-13, 2014, DOI:10.1684/ecn.2014.0348
Abstract Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of ALL cells. LBMp (leukemic bone marrow plasma) at the time of… More >

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ARTICLE

Ewa Ziemann¹, Robert A. Olek², Tomasz Grzywacz¹, Jan J. Kaczor³, Jędrzej Antosiewicz⁴, Wojciech Skrobot³, Sylwester Kujach¹, Radosław Laskowski¹
European Cytokine Network, Vol.25, No.1, pp. 14-23, 2014, DOI:10.1684/ecn.2014.0349
Abstract Inflammation may accompany obesity and a variety of diseases, or result from excessive exercise. The aim of this study was to investigate the anti-inflammatory effect of whole-body cryostimulation on the inflammatory response induced by eccentric exercise under laboratory conditions. The study also sought to establish if cold treatment changes the lipid profile and modifies energy expenditure in young people. Eighteen healthy and physically active, college-aged men volunteered to participate in the experiment. They were divided into two subgroups: CRY-submitted to whole-body cryostimulation, and CONT- a control group. Both groups performed eccentric work to induce muscle… More >

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Gholamreza Daryabor¹, Mahdi Mahmoudi², Ahmadreza Jamshidi², Keramat Nourijelyani³, Aliakbar Amirzargar¹, Nooshin Ahmadzadeh², Elham Farhadi⁴, Mohammad Hossein Nicknam¹
European Cytokine Network, Vol.25, No.1, pp. 24-29, 2014, DOI:10.1684/ecn.2014.0350
Abstract Introduction: The result of recent genome-wide association studies revealed that, in addition to HLAB27, a few non-HLAgenes are associated with susceptibility to ankylosing spondylitis (AS) in Caucasian populations. According to these studies, IL-23R is one of the genes that is associated with AS. In this study, we evaluated five important single nucleotide polymorphisms (SNPs) of the IL-23R gene which confers susceptibility to AS, and its effects on the severity of the disease in HLA-B27 positive and negative patients and several subtypes of HLA-B27. Materials and methods: The study population consisted of 294 AS patients and… More >

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