Open Access

REVIEW

John E. Sims, Dirk E. Smith
European Cytokine Network, Vol.14, No.2, pp. 77-81, 2003
Abstract This article has no abstract. More >

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ARTICLE

Paolo Ruggiero^1,*, Silvio Flati², Vito Di Cioccio¹, Giovanni Maurizi², Giovanni Macchia¹, Alberto Facchin¹, Roberto Anacardio¹, Antonio Maras³, Marilena Lucarelli⁴, Diana Boraschi⁴
European Cytokine Network, Vol.14, No.2, pp. 91-96, 2003
Abstract The human chemokine CCL2 gene was expressed in the yeast P.pastoris and gave rise to a mixture of differently glycosylated recombinant proteins. In comparison to non-glycosylated E.coli-derived CCL2, glycosylated yeast CCL2L was 4-20 times less active in a chemotactic assay in vitro. However, CCL2L could maintain full activity upon prolonged incubation at 37°C, whereas the non-glycosylated chemokine readily lost activity. It could be hypothesized that glycosylation is a mechanism used by the organism to modulate CCL2 stability. The partial loss of specific activity due to glycosylation is balanced by the advantage of prolonging the effectiveness of More >

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ARTICLE

Silvia Di Loreto, Rita Maccarone, Luigi Corvetti, Pierluigi Sebastiani, Daniela Piancatelli, Domenico Adorno
European Cytokine Network, Vol.14, No.2, pp. 97-102, 2003
Abstract We analysed the specific effects of IL-1β immunoneutralization on the expression of IL-6 in different pure cultures of neurones and glia after both experimental subliminal hypoxia and recovery. Whereas the IL-1β-deprivation signal induced a decrease in IL-6 expression and release of normoxic neurones, it provoked an increase in IL-6 protein in hypoxic neurones. Moreover, the direct correlation between IL-1β and IL-6, observed in normal and recovering neuronal cultures, was reversed in hypoxic conditions. These reversals were not observed in glial cells, in which IL-1β immunosuppression led to a decrease in IL-6 under all conditions considered. More >

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ARTICLE

Thomas Hehlgans, Peter Müller, Peter Stopfer, Daniela N. Männel
European Cytokine Network, Vol.14, No.2, pp. 103-107, 2003
Abstract Activation of the lymphotoxin beta-receptor (LTβR), a member of the tumor necrosis factor receptor family, plays a crucial role in lymphoid organogenesis and tumor development. Lymphotoxin a1b2 (LTa1b2) and LIGHT have been identified as membrane anchored ligands for the LTβR. While LTβR is expressed on a wide range of cell types e.g. fibroblasts and monocytes, the ligands are expressed only on activated lymphocytes and NK cells. In order to characterize LTβR expression and the biological consequences of LTβR activation rat anti-mouse LTβR monoclonal antibodies were generated. These antibodies recognized a mouse LTβR-Ig fusion protein as More >

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ARTICLE

John K. A. Tetteh¹, Mark M. Addae¹, Naruhiko Ishiwada², Sena M. Yempewu¹, Shinya Yamaguchi¹, David Ofori-Adjei¹, Hitoshi Kamiya⁴, Yoshihiro Komada³, Bartholomew D. Akanmori^1,*
European Cytokine Network, Vol.14, No.2, pp. 109-113, 2003
Abstract To understand the pathogenesis of vaccine-modified measles (VMM), we measured plasma levels of IFN-γ and IL-2 (Th1 cytokines), IL-4 and IL-10 (Th2 cytokines), IL-12, TNF-α and TGF-β1 in children with uncomplicated measles, who had anti-measles IgG antibodies and with a history of immunization on admission (day 0), day 14 and day 60. We compared these to levels in healthy, age-matched, immunized children. Plasma levels of IFN-γ, IL-2 and IL-12 were significantly higher in VMM patients on day 0 compared to healthy controls (p = 0.023; p = 0.018; p = 0.001) respectively. In contrast, plasma… More >

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MEETING REPORT

Stéphane Bécart, Katia Boniface, Marie Cherrier, Olivia Lanvin, Valérie Soulard, Frédéric Toulza
European Cytokine Network, Vol.14, No.2, pp. 115-119, 2003
Abstract The annual meeting of the Société Française d‘immunologie (SFI) took place in Strasbourg 27‐29th November 2002. The following is a brief synopsis of the key points from presentations in the plenary sessions and symposia, and demonstrates the diversity of subjects addressed in the course of this conference. More >

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