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Frédéric Bloch¹, Bénédicte Charmeteau², Michael Tovey³, Jean-Franc¸ois Meritet⁴, Corinne Desaint^5,6, Odile Launay^4,5,6, Pierre Lebon⁴
European Cytokine Network, Vol.27, No.3, pp. 63-67, 2016, DOI:10.1684/ecn.2016.0378
Abstract Objective: To investigate the relationship between the response to influenza vaccination and the ability to produce proinflamatory cytokines in elderly subjects. Methods: Whole blood samples from 25 elderly subjects collected before influenza vaccination were stimulated with the influenza vaccine in order to evaluate the secretion of five specific cytokines: TNFα, IFNα, IFNγ, IL2 and IL10. The results were correlated with the increased HAI antibody titres two weeks after vaccination.Results:Only 30% of elderly individuals seroconverted after vaccination. Although 50 to 70% of the cohort did not produce TNFα, IFNα, IFNγ, IL2 or IL10, all of the More >

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ARTICLE

Carlos Erik Medina Santos¹, Carmen Nathaly López Hurtado¹, Bruno Rivas Santiago¹, Roberto Gonzalez-Amaro³, Yolanda Guadalupe Cataño Cañizales¹, Margarita de la Luz Martínez Fierro², José Antonio Enciso-Moreno¹, Mariana Haydee García Hernández¹
European Cytokine Network, Vol.27, No.3, pp. 68-74, 2016, DOI:10.1684/ecn.2016.0379
Abstract The aim of this study was to evaluate the effects of the LL-37, HNP-1 and HBD2/3 peptides on cytokine and MMP production in human polymorphonuclear cells, mononuclear cells and chondrocytes. The levels of cytokines in supernatants from mononuclear and polymorphonuclear cell cultures were measured with a cytometric bead array by flow cytometry. Likewise, the levels of metalloproteinase/MMP-1, 3, and 13 were measured in supernatants from chondrocyte cultures using an ELISA. The expression of RANKL on lymphocytes was analyzed by flow cytometry. We observed increased levels of TNF-α, IL-6 and IL-10 in mononuclear and polymorphonuclear cell… More >

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ARTICLE

Sarah Lindberg, Moncef Zarrouk, Jan Holst, Anders Gottsäter
European Cytokine Network, Vol.27, No.3, pp. 75-80, 2016, DOI:10.1684/ecn.2016.0381
Abstract Purpose: Inflammation with leukocytic infiltration, degradation of extracellular matrix (ECM), and depletion of vascular smooth muscle cells (VSMC) are pathological hallmarks of abdominal aortic aneurysm (AAA). The aim of this study was to further evaluate relationships between AAA and inflammatory biomarkers, interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), endothelin-1 (ET-1) and soluble urokinase-type plasminogen activator receptor (suPAR), by comparing levels in 65-year-old men with and without AAA at ultrasound screening. We also evaluated whether any biomarker can independently predict AAA at screening, and clarified potential correlations between aortic diameter and blood levels of these biomarkers. Results:… More >

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