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Mahboobeh Razmkhah¹, Mansoureh Jaberipour¹, Akbar Safaei², Abdol-Rasoul Talei³, Nasrollah Erfani¹, Abbass Ghaderi^1,4
European Cytokine Network, Vol.23, No.3, pp. 72-77, 2012, DOI:10.1684/ecn.2012.0310
Abstract Background: Lymph nodes (LNs) are among the first sites of tumor metastasis. The expression of chemokines and chemokine receptors in LNs are involved in cancer prognosis and are considered to be good predictors of tumor progression. The main aim of this study was to assess the expression of important, tumorpromoting chemokines and chemokine receptors in LNs of breast cancer patients. Methods: LNs were isolated from eighteen women diagnosed with breast cancer. Data were compared between positive and negative LNs. Expression of chemokines and chemokine receptors were determined by quantitative real-time PCR (qRT-PCR) and flow cytometry.… More >

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ARTICLE

Nehal M. Elsherbiny¹, Khaled H. Abd El Galil², Mahmoud M. Gabr³, Mohammed M. Al-Gayyar¹, Laila A. Eissa¹, Mamdouh M. El-Shishtawy¹
European Cytokine Network, Vol.23, No.3, pp. 78-86, 2012, DOI:10.1684/ecn.2012.0309
Abstract Diabetic nephropathy (DN) remains the most common cause of end-stage renal disease. Although, adenosine acts as a local modulator with a cytoprotective function, extracellular adenosine usually disappears quickly due to a rapid uptake into adjacent cells. Therefore, we investigated the effect of 5' -(N-ethylcarboxamido)- adenosine (NECA), a stable, nonselective adenosine receptor agonist, on diabetes-induced increases in inflammatory cytokines and adhesion molecules. The enhancement of adenosine receptor action by NECA was examined in the renal tissues of rats with streptozotocin-induced diabetes. Daily i.p. injections of NECA at 0.3 mg/kg/day were given to rats, over a two-week… More >

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ARTICLE

Sonia Ben-Hadj-Khalifa^1,2, Philippe Nguyen^2,3, Touhami Mahjoub¹, Nathalie Hézard^2,3
European Cytokine Network, Vol.23, No.3, pp. 87-92, 2012, DOI:10.1684/ecn.2012.0315
Abstract Background: Monocytes and factor Xa (FXa) are procoagulant agents implicated in the physiopathological processes of atherosclerosis and thrombosis.Objective: we evaluated the anticoagulant effect of the anti-inflammatory cytokine IL-10 on an FXa-activated human monocyte (Hu-monocyte) procoagulant phenotype.Methods: Hu-monocytes were purified by elutriation and activated by FXa. The effect of IL-10 was assessed by means of a 2 h pre-incubation step with recombined human IL-10 (0.5 and 1 ng/mL). Real-time RT-PCR and Western blotting were used to evaluate the effect of IL-10 on tissue factor (TF) mRNA and protein synthesis. A thrombin generation (TG) assay was used… More >

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ARTICLE

Ewa Jabłońska¹, Natalia Wawrusiewicz-Kurylonek², Marzena Garley¹, Wioletta Ratajczak-Wrona¹, Bożena Antonowicz³, Dorota Dziemiańczyk-Pakieła⁴, Jakub Jabłoński⁵, Adam Krętowski², Stanisława Zyta Grabowska⁴
European Cytokine Network, Vol.23, No.3, pp. 93-100, 2012, DOI:10.1684/ecn.2012.0311
Abstract Available data indicating a role for neutrophils in the tumor-host reactions are controversial. In 37 patients with oral cavity squamous cell carcinoma (OSCC), we investigated the expression of a tumor-promoting, proliferation-inducing ligand (APRIL) molecule by peripheral blood neutrophils isolated from blood samples collected at presentation and three weeks after surgery, and the serum levels of TGF-in the same samples. Additionally, we investigated the consequences of TLR4 activation by LPS for the synthesis of APRIL by those cells.The levels of mRNA for APRIL and TLR4 were measured using a real-time PCR method. Western blot analysis was… More >

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ARTICLE

Maha Kammoun-Krichen^1,2, Noura Bougacha-Elleuch^1,2, Mouna Mnif^1,3, Fadia Bougacha⁴, Ilhem Charffedine⁵, Sandra Rebuffat^1,6, Ahmed Rebai⁴, Emilie Glasson⁷, Mohamed Abid³, Fatma Ayadi⁸, Sylvie Péraldi-Roux^1,6, Hammadi Ayadi^1,2
European Cytokine Network, Vol.23, No.3, pp. 101-106, 2012, DOI:10.1684/ecn.2012.0312
Abstract Interactions between cytokines and others soluble factors (hormones, antibodies...) can play an important role in the development of thyroid pathogenesis.The purpose of the present study was to examine the possible correlation between serum cytokine concentrations, thyroid hormones (FT4 and TSH) and auto-antibodies (Tg and TPO), and their usefulness in discriminating between different thyroid conditions. In this study, we investigated serum from 115 patients affected with a variety of thyroid conditions (44 Graves’ disease, 17 Hashimoto’s thyroiditis, 11 atrophic thyroiditis, 28 thyroid nodular goitre and 15 papillary thyroid cancer), and 30 controls. Levels of 17 cytokines More >

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ARTICLE

Nada Alaaeddine¹, Joseph Sidaoui¹, George Hilal¹, Reem Serhal¹, Abir Abedelrahman², Salem Khoury³
European Cytokine Network, Vol.23, No.3, pp. 107-111, 2012, DOI:10.1684/ecn.2012.0313
Abstract Background and aim: tumor necrosis factor (TNF)-α plays a significant role in the pathogenesis of nonalcoholic steatohepatitis (NASH). A few studies have confirmed high TNF-α plasma protein levels in patients with NASH compared to healthy volunteers. We herein aimed to revisit these findings using other molecular techniques. Methods: a cross-sectional evaluation of patients newly diagnosed with NASH. A quantitative assay for the measurement of TNF-α messenger ribonucleic acid (mRNA) was performed for NASH patients and controls using real-time reverse transcription polymerase chain reaction (RT-PCR). Results: in 39 patients with NASH (mean age 38.6 ± 9.4 More >

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Dalila Mezioug, Chafia Touil-Boukoffa
European Cytokine Network, Vol.23, No.3, pp. 112-119, 2012, DOI:10.1684/ecn.2012.0314
Abstract Hydatidosis is a parasitic disease caused by the development, in humans and other mammals, of the larval form of Taenia,Echinococcus granulosus. It is one of the world’s major zoonotic infections. This study aimed to examine interleukin-6 (IL-6), interferon-γ (IFN-γ) and interleukin-17A (IL-17A) production in patients with cystic echinococcosis (CE), and the role of IL-17A in the modulation of the immune response against the extracellular parasite, E. granulosus. A relationship between IL-6, IL-17A production and C reactive Protein (CRP) levels was also assessed. IL-6, IFN-γ, IL-17A and CRP production were determined in serum from Algerian hydatid… More >

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