Open Access

REVIEW

Mariette Lisbonne, Maria C. Leite-de-Moraes^*
European Cytokine Network, Vol.14, No.1, pp. 4-14, 2003
Abstract This article has no abstract. More >

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ARTICLE

Vincent L. Butty¹, Pascale Roux-Lombard², Jorge Garbino³, Jean-Michel Dayer², Bara Ricou¹, and the Geneva Sepsis Network¹ (participating members are listed in the appendix)
European Cytokine Network, Vol.14, No.1, pp. 15-19, 2003
Abstract Objectives: To investigate the effects of Lenercept®, a recombinant soluble TNF receptor p55 fused to an immunoglobulin heavy chain IgG₁, on the balance of pro- and anti-inflammatory mediators in sepsis.
Design: Post hoc analysis of a subgroup of patients enrolled in a multicenter phase III, prospective, double-blind, placebo-controlled, randomized study of Lenercept ® in severe sepsis.
Setting: Surgical and medical intensive care units, and postoperative recovery room of a tertiary care teaching hospital.
Patients: A total of 57 patients were enrolled in the multicenter study in our center.
Intervention: Septic patients were randomly assigned to receive either Lenercept… More >

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ARTICLE

Laura Brandolini¹, Marcello Allegretti¹, Valerio Berdini¹, Maria Neve Cervellera¹, Patrizia Mascagni¹, Matteo Rinaldi¹, Gabriella Melillo¹, Pietro Ghezzi², Manuela Mengozzi², Riccardo Bertini^1,*
European Cytokine Network, Vol.14, No.1, pp. 20-26, 2003
Abstract Carbocysteine lysine salt monohydrate (SCMC-Lys) is a well-known mucoactive drug whose therapeutic efficacy is commonly related to the ability of SCMC-Lys to replace fucomucins by sialomucins. The aim of this study was to determine if SCMC-Lys could exert an anti-oxidant action by scavenging reactive oxygen intermediates (ROIs). Our results show that SCMC-Lys proved effective as a selective scavenger of hypochlorous acid (HOCl) and hydroxyl radical (OH• ), this effect being related to the reactivity of the SCMC tioether group. The scavenger activity of SCMC-Lys was observed in free cellular system as well as in activated More >

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ARTICLE

Mahmoud Huleihel^1,2,4,*, Eitan Lunenfeld^2,4, Anna Blindman¹, Isebrand Prinsloo^3,4, Gad Potashnik^2,4
European Cytokine Network, Vol.14, No.1, pp. 27-33, 2003
Abstract The levels of IL-1α, IL-1β and IL-1Ra were higher in homogenates of testicular tissue from sexually immature than those from mature mice. Immunohistochemical staining of testicular tissues from sexually immature and adult mice show that differentiated germ cells express higher levels of IL-1α compared to Sertoli cells and Leydig cells/interstitial cells. Peritubular cells of sexually immature and adult mice did not express IL-1α. Testicular tissue cells of adult mice showed high levels of expression of IL-1β, mainly in the cytoplasm and nucleus of the spermatogonia and in spermatocytes. Sertoli cells and Leydig/interstitial cells were also… More >

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ARTICLE

Trawat Attarbaschi¹, Martin Willheim², Michael Ramharter¹, Andrea Hofmann³, Katharina Wahl², Heidi Winkler¹, Wolfgang Graninger¹, Stefan Winkler^1,*
European Cytokine Network, Vol.14, No.1, pp. 34-39, 2003
Abstract Cytokine profiles of CD4⁺ and CD8⁺ T-cell subsets were evaluated in 8 patients with infectious mononucleosis (IM). Intracellular detection of cytokines using flow cytometry revealed an expansion of IFN-γ-expressing CD4⁺ T cells, and particularly CD8⁺ T cells, while IL-2 expressing cells were less frequently encountered when compared to healthy controls. Single TNF-α-expressing CD4⁺ and CD8⁺ T cells were likewise reduced and shifted towards IFN-γ/TNF-α co-production. The predominant pro-inflammatory type 1-biased immune response during IM was emphasized by low frequencies of IL-10 expression in both T cell subsets, although some patients displayed elevated serum levels. Six months later, a… More >

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ARTICLE

Halina Urbañska-Rys´, Agnieszka Wierzbowska, Tadeusz Robak
European Cytokine Network, Vol.14, No.1, pp. 40-51, 2003
Abstract We investigated the serum concentrations of selected angiogenic cytokines including: vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) and basic fibroblast growth factor (bFGF) in 162 patients with multiple myeloma (MM), 5 patients with Waldenström’s macroglobulinaemia (WM), and 31 healthy controls. Among the MM patients there were 2 cases of primary plasma cell leukemia (PCL) and one case of extramedullary plasmacytoma. The levels of measured cytokines were correlated with the phase and stage of the disease as well as the most important clinical and laboratory parameters associated with… More >

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ARTICLE

Diane Damotte^1,3, Claire Goulvestre², Jeannine Charreire², Claude Carnaud¹
European Cytokine Network, Vol.14, No.1, pp. 52-59, 2003
Abstract Although adjuvants are essential for the initiation of experimental autoimmune diseases, their precise contribution to the pathological manifestations is still poorly understood. Experimental autoimmune thyroiditis (EAT) is interesting in that respect because it can be initiated with the help of two different adjuvants — Freund’s complete or LPS — which may initiate independent pathogenic pathways. In the present study, we have compared Freund’s-induced versus LPS-induced EAT with respect to their dependence upon CD8⁺ T cells, which are considered as major actors in the pathogenesis of thyroiditis. Our results reveal that whereas CD8+ T cells are mandatory More >

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ARTICLE

Séverine Froget¹, Emmanuelle Barthelemy², François Guillot¹, Christophe Soler¹, Marie-Claude Coudert², Marc Benbunan², Christine Dosquet^2,3
European Cytokine Network, Vol.14, No.1, pp. 60-64, 2003
Abstract Cell and tissue therapy applications in humans are being used increasingly, particularly for tissue repair. Several reconstructed skin models have been proposed. Wound healing involves overlapping steps of inflammation, cell migration and proliferation, neovascularisation, extracellular matrix production and remo-delling. This is regulated by numerous cytokines and other soluble mediators. We have prepared dermal substitutes (DS) consisting of a collagen-GAG, three-dimensional matrix colonized by human dermal fibroblasts (HDF), isolated by skin explant or enzymatic digestion of the skin for potential therapeutic use in humans. To test the functionality of these DS, we measured (ELISA) the stimulatory… More >

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ARTICLE

M. D. Rodríguez-Arnao, A. Rodríguez-Sánchez, J. Rodríguez-Arnao, E. Dulín-Íñiguez¹, J. M. Bellón Cano², M. A. Muñoz-Fernández²
European Cytokine Network, Vol.14, No.1, pp. 63-68, 2003
Abstract Objetive: To analyse the production of TNF-α and NO in euthyroid and hypothyroid newborns.
Patients: A cross-sectional study was conducted involving 10 newborns diagnosed with primary congenital hypothyroidism (CH; group A) and 10 euthyroid children (group B).
Results: There were undetectable plasma levels of TNF-α and NO in the hypothyroid children, however plasma levels of TNF-α (5.5 ± 0.5 pg/ml) and NO (5.6 ± 1.7 µM) were detected at normal levels in all euthyroid children. Moreover, expression of iNOS mRNA in PBMC, determined by RT-PCR, was negative in both groups of infants.
Conclusion: TNF-α and NO production More >

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ERRATUM

European Cytokine Network, Vol.14, No.1, pp. 66-66, 2003
Abstract This article has no abstract. More >

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