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Eduardo Tamayo^1,2,*, Ana Fernández^1,2,*, Raquel Almansa^2,3,*, Elena Carrasco^1,2, María Heredia^1,2, Carmen Lajo^1,2, Lisbeth Goncalves^2,3, Jose I. Gómez-Herreras^1,2, Raúl Ortiz de Lejarazu², Jesus F. Bermejo-Martin^2,3,4
European Cytokine Network, Vol.22, No.2, pp. 82-87, 2011, DOI:10.1684/ecn.2011.0281
Abstract The relationships between cytokine responses in septic shock are currently poorly understood. Some studies have pointed to a biphasic model, with an initial proinflammatory phase, followed by a reactive, antiinflammatory response to explain the pathogenesis of the most severe form of sepsis. However, evidence for the coexistence of both responses has been found. In this study, the plasma levels of 17 cytokines and chemokines, in 20 patients with septic shock, 11 patients with systemic inflammatory response syndrome (SIRS), during the first 24 hours following diagnosis, and 10 healthy controls, were analyzed and compared. Patients with… More >

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ARTICLE

Arsene Mekinian¹, Ryad Tamouza², Stephan Pavy³, Nicolas Gestermann¹, Marc Ittah¹, Xavier Mariette^1,3, Corinne Miceli-Richard^1,3
European Cytokine Network, Vol.22, No.2, pp. 88-102, 2011, DOI:10.1684/ecn.2011.0285
Abstract The functional consequences of TNF-α promoter SNPs are still controversial and, to date, the functional consequences of TNF-α haplotype combinations in healthy subjects have not been assessed. In order to assess functional consequences of each TNF-α polymorphism and of their haplotype combination, TNF-α expression and secretion by LPS-stimulated monocytes from 50 healthy subjects were assessed. Monocytes were isolated and cultured for four hours, after 100 ng/mL LPS stimulation. mRNA expression was quantified using the real-time polymerase chain reaction, and TNF-α levels were measured by enzyme-linked immunosorbent assay. Each subject was genotyped for TNF-α -857 C/T,… More >

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Ali Hossein Mesgarzadeh¹, Ali Akbar Abolfathi², Saeed Dastgiri³, Maghsod Shaaker^2,4, Amir Mansour Vatankhah⁵, Shiva Solehakahnamoiee¹, Masoud Darabi²
European Cytokine Network, Vol.22, No.2, pp. 103-106, 2011, DOI:10.1684/ecn.2011.0286
Abstract The clinical evaluation and management of impacted third molars remain challenging. The aim of the present study was to investigate possible associations between follicular tissue cytokines and radiographic manifestations of impacted third molar. The population included 72 patients who underwent surgical extraction of impacted third molars. All these patients underwent a preliminary panoramic radiograph. Levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and transforming growth factor beta (TGF-β) in tissue extracts were determined using ELISA. There were no significant differences between bony and tissue impaction as regards IL-1β, IL-6 and TGF-β levels. Moreover, the same results More >

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Afsaneh Morteza, Manouchehr Nakhjavani, Akram Ghadiri-Anari, Alireza Esteghamati, Omid Khalilzadeh
European Cytokine Network, Vol.22, No.2, pp. 107-112, 2011, DOI:10.1684/ecn.2011.0282
Abstract Introduction. In vitro studies have shown that oxidized, low density lipoprotein (ox-LDL) stimulates macrophages to release interleukin-6 (IL-6) and interleukin-1β (IL-1β). In this study, we aimed to investigate the correlation between ox-LDL and IL-6 and IL-1β levels in the peripheral circulation of patients with type 2 diabetes, and normal controls. We measured serum high sensitivity CRP (hs-CRP) levels in order to define basal inflammation in patients and controls. Methods. A total of 40 patients with type 2 diabetes, and 40, age, sex, and body mass index (BMI) -matched healthy adults were enrolled in the study.… More >

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Ferhat Cekmez¹, Fuat Emre Canpolat¹, Merih Çetinkaya¹, Seçil Aydinöz², Gokhan Aydemir², Ferhan Karademir², Osman Metin Ipcioglu³, Serdar Ümit Sarici¹
European Cytokine Network, Vol.22, No.2, pp. 113-117, 2011, DOI:10.1684/ecn.2011.0283
Abstract Aim. The aim of this study was to evaluate the predictive value of resistin and visfatin in neonatal sepsis, and to compare these adipocytokines with C-reactive protein (CRP), procalcitonin and interleukin 6 (IL-6). Donors and methods. A total of 62 term or near term infants with sepsis proven by positivity of blood culture, and 43 healthy infants were included in this study. Results. There were no statistically significant differences between the two groups as regards birthweight and gestational age. White blood cell count (p= 0.039), CRP levels (p=0.01), procalcitonin levels (p=0.01), IL-6 levels (p= 0.01),… More >

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Cihan Meral¹, Ferhat Cekmez², Sebahattin Vurucu², Emre Tascılar², Ozgur Pirgon¹, F. Emre Canpolat², Osman Metin Ipcioglu³, Gokhan Aydemir¹, Secil Aydınoz¹
European Cytokine Network, Vol.22, No.2, pp. 118-122, 2011, DOI:10.1684/ecn.2011.0284
Abstract Aim. To investigate the relationship between the newly discovered adipocytokines and increasing body weight (paralleled by increased insulin resistance), and antiepileptic drug therapy with valproic acid (VPA). Design and methods. 44 children with idiopathic, generalized epilepsy treated with valproic acid (VPA), and 40 control group children were included in this study. Results. Both the VPA-treated group and the control group showed no significant difference in terms of age, total cholesterol and LDL-cholesterol. Subjects in the VPA group had significantly higher BMI-SDS than control subjects (2.3±0.15 vs-0.04±0.8, p<0.001). HOMA-IR, apelin and visfatin levels were significantly increased More >

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Safia Messaoudi¹, Ghada M. Al-Khateeb², Maryam Dendana¹, Mai S. Sater², Khaled B. Jazia³, Mounia Nouira⁴, Wassim Y. Almawi², Touhami Mahjoub¹
European Cytokine Network, Vol.22, No.2, pp. 123-126, 2011, DOI:10.1684/ecn.2011.0287
Abstract Objectives. Insofar as recurrent spontaneous miscarriage (RSM) is linked with dysregulated immunity and inflammatory changes, and given the pro-inflammatory role of interleukin-21 (IL-21), we examined the association between IL-21 polymorphisms and RSM. Methods and results. IL-21 rs2055979, rs13143866, rs9992580, and rs4833837 were genotyped in 235 cases of RSM and 235 controls. Regression analysis was employed in assessing the contribution of IL-21 variants to the overall RSM risk. Higher minor allele and genotype frequencies of rs2055979 and rs13143866, but not rs9992580 or rs4833837, were seen in RSM patients than in the controls. IL-21 haplotype [rs9992580/rs4833837/rs2055979/rs13143866] analysis More >

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