Open Access

ARTICLE

Ozbey G¹, Gorczynski R², Erin N¹
European Cytokine Network, Vol.25, No.2, pp. 30-34, 2014, DOI:10.1684/ecn.2014.0353
Abstract Measurements of cytokines in cell culture supernatants are widely used to evaluate the immune response. Cytokine levels in secretomes are usually quantified using enzyme-linked immunosorbent assays (ELISA), which have easy, sensitive, specific, rapid, cost-effective, and reproducible protocols. To our knowledge, the stability of cytokines in secretomes has not been hitherto investigated. We present data that involve; time-dependent changes during storage at +4℃, and the effects of freeze-thaw cycles in samples frozen at -80℃, instant freezing of samples with liquid nitrogen, and addition of protease inhibitors on the stability of certain cytokines (TNF-α, MIP-2, IFN-γ, IL-6, More >

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Open Access

ARTICLE

Vahid Ziaee^1,2, Fatemeh Tahghighi^1,2, Mohammad Hassan Moradinejad², Sara Harsini³, Maryam Mahmoudi⁴, Arezou Rezaei³, Samaneh Soltani⁵, Maryam Sadr⁵, Yahya Aghighi⁶, Nima Rezaei^2,3,5
European Cytokine Network, Vol.25, No.2, pp. 35-40, 2014, DOI:10.1684/ecn.2014.0352
Abstract Background: Juvenile systemic lupus erythematosus (JSLE) is a polygenic, autoimmune disorder of unknown origin. As proinflammatory cytokines, including interleukin-6 (IL-6) and the interleukin-1 (IL-1) family, seem to contribute to the pathogenesis of JSLE, this investigation was performed to assess the associations of particular single nucleotide polymorphisms (SNPs) of IL-6 and IL-1 genes in a case-control study. Methods: Fifty nine JSLE cases were recruited for this study as the patient group, and were compared against 140 healthy, unrelated, control subjects. Using the polymerase chain reaction with the sequence-specific primer method, genotyping was carried out for the… More >

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