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Fatemeh Mohammadi¹, Ghasem Solgi², Maryam Tajik¹, Pedram Ahmadpoor³, Hasan Nikoeinejad⁴, Behzad Einollahi⁴, Banafsheh Nazari¹, Mahboob Lessan-pezeshki⁵, Aliakbar Amirzargar^1,6
European Cytokine Network, Vol.28, No.3, pp. 93-101, 2017, DOI:10.1684/ecn.2017.0397
Abstract The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for meanly 3 years post-transplantation. Blood samples were collected pre-transplant, 2, 4 and 12 weeks post-transplant. The frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined in all time-points using ELISPOT assay. The results of ELISpot monitoring and levels of IL-23 and TGF-β were compared between recipients with acute (n = 12) or… More >

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ARTICLE

Alba Martínez^1,2, Cristina Bono^1,2, Javier Megías³, Alberto Yánez˜^4,5, Daniel Gozalbo^1,2, M. Luisa Gil^1,2
European Cytokine Network, Vol.28, No.3, pp. 102-110, 2017, DOI:10.1684/ecn.2017.0398
Abstract Toll-like receptor (TLR) agonists drive hematopoietic stem and progenitor cells (HSPCs) to differentiate along the myeloid lineage in vitro and also in vivo following infection. In this study, we used an in vitro model of HSPC differentiation to investigate the functional consequences (cytokine production) that exposing HSPCs to various pathogen-associated molecular patterns (PAMPs) and Candida albicans cells have on the subsequently derived macrophages. Mouse HSPCs (Lin– cells) were cultured with GM-CSF to induce macrophage differentiation in the presence or absence of the following pattern recognition receptor (PRR) agonists: Pam₃CSK₄ (TLR2 ligand), LPS (TLR4 ligand), depleted zymosan (which only activates Dectin-1),… More >

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ARTICLE

José L. Aparicio¹, Macarena Ottobre¹, Maite Duhalde Vega¹, Jean-Paul Coutelier², Jacques Van Snick³, Lilia A. Retegui¹
European Cytokine Network, Vol.28, No.3, pp. 111-119, 2017, DOI:10.1684/ecn.2017.0399
Abstract Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouseMHV-A59-infection.MAb anti-IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59- infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not More >

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ARTICLE

Lihua Zhu^1,a, Liyan Zhu^2,a
European Cytokine Network, Vol.28, No.3, pp. 120-126, 2017, DOI:10.1684/ecn.2017.0400
Abstract Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting nearly 1% of adults worldwide. This study aimed to investigate whether sophocarpine is a potential drug for treating RA. Methods: The cytotoxicity of sophocarpine to RA-fibroblast-like synoviocytes (FLSs) was evaluated using 3-[4,-dimethylthiazol2-y]-2,5-diphenyl-tetrazolium bromide (MTT) assays kit and released lactate dehydrogenase (LDH) assays. The transcription of proinflammatory cytokines in RA-FLSs was analyzed by reverse transcription and real-time polymerase chain reaction (RT-PCR). The proteins levels were further verified by enzyme-linked immunosorbent assay (ELISA). The alterations in the mediators of mitogen-activated protein kinase (MAPK) and nuclear factor B… More >

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