Open Access

ARTICLE

Julie G. Burel^1,2, Simon H. Apte¹, Denise L. Doolan^1,2
European Cytokine Network, Vol.26, No.4, pp. 67-72, 2015, DOI:10.1684/ecn.2015.0369
Abstract Polyfunctional T cells that simultaneously produce the cytokines IFN-γ, IL-2 and TNF have been correlated with better clinical outcomes in various diseases. To date, cytokine polyfunctionality within T cells has been exclusively studied by intracellular cytokine staining coupled with flow cytometric analysis. Thus, further downstream interrogation of polyfunctional T cell characteristics such as transcriptomic analysis has not been possible. Here, we report the use of a flow cytometric method based on cytokine secretion assay technology to detect and isolate, for the first time, viable human polyfunctional T cells directly from in vitro stimulated whole blood More >

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Open Access

ARTICLE

Ozen Oz Gul¹, Soner Cander², Bulent Gul³, Ebru Açıkgoz⁴, Emre Sarandol⁴, Canan Ersoy¹
European Cytokine Network, Vol.26, No.4, pp. 73-78, 2015, DOI:10.1684/ecn.2015.0370
Abstract This study was designed to evaluate insulin resistance and plasma levels of visfatin and resistin in obese and non-obese patients with polycystic ovary syndrome (PCOS).A total of 37 premenopausal PCOS patients with (n = 18, mean (SD) age: 27.5 (5.7 years) or without obesity (n = 19, mean (SD) age: 23.7 (3.1) years) and healthy volunteers (n = 18, mean (SD) age:29.8 (4.1) years) were included in this study. Data on clinical characteristics, glycemic parameters and lipid parameters were recorded for each subject as were plasma visfatin and resistin levels. Mean (SD) HOMA-IR values were… More >

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