Guest Editors
Dr. Federico Antonio Gutiérrez-Miceli, Tecnológico Nacional de México / IT Tuxtla Gutiérrez, Mexico
Dr. Nancy Ruíz Lau, Tecnológico Nacional de México / IT Tuxtla Gutiérrez, Mexico
Dr. Maria Celina Lujan Hidalgo, Tecnológico Nacional de México / IT Tuxtla Gutiérrez, Mexico
Dr. Carlos Arias Castro, Instituto Tecnológico de Tlajomulco, Mexico
Summary
The Secondary metabolites produced by plants have acquired an important presence in the development of process for special usage in the pharmaceutical, cosmetic, and other industries. In recent years, the usage of plant secondary metabolites has increased, especially for degenerative diseases such as diabetes, cancer, Alzheimer and a numerous of viral infections.
The objective of this special issue is to increase the knowledge of the biotechnology techniques that are being used to produce secondary metabolites in diverse plant species. In vitro systems such as callus and cell suspension cultures can be published as experimental systems for secondary metabolites production. The techniques for genetic modifications and stress biotic and abiotic can be used for the study of the increase production effect of secondary metabolites. The biotic stress such as microorganisms which have interaction either endogenous or exogenous are an important topic to have further research. Abiotic stress such as UV, laser radiation, drought, and heavy metals are also very important.
Keywords
Secondary Metabolites; Plant Cell Culture; Callus Culture; Genetic Modification; Abiotic Stress; Biotic Stress
Published Papers
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Open Access
ARTICLE
Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic
Wei Tian, Lingdi Liu, Zhongxing Song, Zhishu Tang, Chunxiu Wen, Tao Jiang
Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 369-382, 2023, DOI:10.32604/phyton.2022.023184
(This article belongs to this Special Issue:
Biotechnology of Plant Secondary Metabolites)
Abstract The extract from
Semen Ziziphi Spinosae (SZS) contains many secondary metabolites (flavonoids, alkaloids, and terpenoids), widely reported for their high medicinal value. In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS, we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic. A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS. The main flavonoid metabolic components of SZS were catechin, L-epicatechin, (–)-epigallocatechin, (+)-gallocatechin, spinosin…
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Open Access
REVIEW
Plant-Derived Enzymes Producing Chiral Aroma Compounds and Potential Application
Fang Dong, Qian Fan, Xinguo Su, Lanting Zeng
Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 383-398, 2023, DOI:10.32604/phyton.2022.023185
(This article belongs to this Special Issue:
Biotechnology of Plant Secondary Metabolites)
Abstract Aroma (volatile) compounds play important ecological functions in plants, and also contribute to the quality of plant-derived foods. Moreover, chiral aroma compounds affect their functions in plants and lead to different flavor quality properties. Formations of chiral aroma compounds are due to the presence of enzymes producing these compounds in plants, which are generally involved in the final biosynthetic step of the aroma compounds. Here, we review recent progress in research on the plant-derived enzymes producing chiral aroma compounds, and their changes in response to environmental factors. The chiral aroma enzymes that have been reported produce (
R)-linalool, (
S)-linalool, (
R)-limonene, and…
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Open Access
ARTICLE
The Effect of Methylation Modification of MDD on the Expression of Key Genes in the Process of Saponin Synthesis in Eleutherococcus senticosus
Minghui Cui, Limei Lin, Jie Zhang, Xin Song, Shuo Wang, Jing Dong, Xuelei Zhao, Yuehong Long, Zhaobin Xing
Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2649-2668, 2022, DOI:10.32604/phyton.2022.021631
(This article belongs to this Special Issue:
Biotechnology of Plant Secondary Metabolites)
Abstract Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in
Eleutherococcus senticosus. The results of bisulfite sequencing showed that there were three kinds of samples with a low (0.68%), medium (0.72%) and high (0.79%) DNA methylation ratio in the promoter of
MDD in
E.
senticosus, respectively. The transcriptome sequencing results showed that the expression of
MDD in
E.
senticosus was significantly up-regulated in the types with low DNA methylation ratios of
MDD (
P < 0.05). There was a significant negative correlation between the saponin content in
E.
senticosus and the DNA methylation ratio of
MDD…
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Open Access
ARTICLE
In vitro Germination and Micropropagation of Aconitum vilmorinianum: An Important Medicinal Plant in China
Zongmin Mou, Fei Ye, Fangchen Shen, Dake Zhao
Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2331-2348, 2022, DOI:10.32604/phyton.2022.021273
(This article belongs to this Special Issue:
Biotechnology of Plant Secondary Metabolites)
Abstract Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation
and environment disturbance. This study was conducted to provide propagation methods through
in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L
−1 6-benzylaminopurine (BAP), 0.1 mg L
−1 1-napthaleneacetic acid (NAA), and 30 g L
−1
sucrose. Three bacteria (
Pantoea agglomerans,
Erwinia persicina, and
Pseudomonas tolaasii) would be responsible
for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The
influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated…
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