Phyton-International Journal of Experimental Botany

ISSN: 0031-9457 (print) Impact Factor: 1.039
ISSN: 1851-5657 (online) Publication Frequency: Monthly

About the Journal

Phyton-International Journal of Experimental Botany is an international journal that publishes research on all aspects of plant science. This journal welcomes original and exciting submissions that provide new and key insights into the origins, growth and development of plants from the molecular to the whole organism and its interactions with the biotic and abiotic environments.

Indexing and Abstracting

Thomson Scientific; Science Citation Index Expanded (SCIE); Journal Citation Report/Science Edition (JCR); Impact Factor (2020): 1.039; 5-Year Impact Factor: 0.77; Biological Abstracts; BIOSIS Previews; Scopus; EMBiology; Latindex; Field Crop Abstracts; CAB Abstracts; CABI Full Text; Periódica; TEEAL, The Essential Electronic Agricultural Library; Núcleo Básico de Revistas Científicas Argentinas (Agosto 2013–Julio 2015); SciELO (Scientific Electronic Library Online); Portal de Revistas en Biodiversidad; Portico, etc...

Current Issue - Vol.91, No.2, 2022 Most Viewed

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REVIEW
Chlorogenic Acid Metabolism: The Evolution and Roles in Plant Response to Abiotic Stress
Deka Reine Judesse Soviguidi^1,2, Rui Pan¹, Yi Liu^1,2, Liping Rao^1,2, Wenying Zhang^1,*, Xinsun Yang^2,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 239-255, 2022, DOI:10.32604/phyton.2022.018284
Abstract During the evolution, plants acquired the ability to synthesize different phenylpropanoid compounds like chlorogenic acid (CGA), which plays vital roles in resistance mechanisms to abiotic stresses. These environmental factors, including heavy metal, cold, heat, ultraviolet (UV) light, drought, and salinity affect the plant physiological processes, resulting in massive losses of agriculture production. As plants evolve from green algae to bryophytes, ferns, gymnosperms and angiosperms, phenylpropanoids are produced and accumulated in different tissues, giving the plant the capacity to counteract the harmful effects of the adverse environments. Studies have been performed on the metabolic evolution of rosmarinic acid, flavonoids and lignin,… More
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REVIEW
Mycorrhiza and Phosphate Solubilizing Bacteria: Potential Bioagents for Sustainable Phosphorus Management in Agriculture
Fazli Wahid¹, Muhammad Sharif², Shah Fahad³, Amjad Ali⁴, Muhammad Adnan¹, Rafiullah¹, Shah Saud⁵, Subhan Danish^6,*, Muhammad Arif Ali⁶, Niaz Ahmed⁶, Hüseyin Arslan⁷, Doğan Arslan⁸, Murat Erman⁸, Ayman EL Sabagh^8,9,*, Fatemeh Gholizadeh¹⁰, Rahul Datta¹¹
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 257-278, 2022, DOI:10.32604/phyton.2022.016512
Abstract Phosphorus (P) is a critical nutrient that plays an essential role in improving soil fertility for optimum plant growth and productivity. It is one of the most deficient macro-nutrients in agricultural soils after nitrogen and is considered inadequate for plant growth and production. To P availability in soils, the farmers are applying huge amounts of synthetic P fertilizers that adversely affect the wider environment, groundwater, soil fertility and microbial population. Many beneficial microbes are known to release and supply soluble P for improving growth and yield of a variety of plants in a sustainable manner in P deficient soils. Thus,… More
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ARTICLE
Identification of Genes Involved in Celastrol Biosynthesis by Comparative Transcriptome Analysis in Tripterygium wilfordii
Yaru Zhu^1,2,3, Xiujun Zhang¹, Yansheng Zhang³, Shiyou Lü^1,4,*, Changfu Li^1,3,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 279-291, 2022, DOI:10.32604/phyton.2022.017625
Abstract Tripterygium wilfordii has been renowned mostly because of the anticancer effects of its root extracts, which is partly ascribed to the presence of celastrol, a pentacyclic triterpenoid, as one of the main active components. Celastrol also has recently been reported as an effective prodrug in the treatment of obesity. Despite the promising activities, the pathway leading to celastrol biosynthesis, especially cytochrome P450 (CYP) enzyme(s) that occur in its downstream steps, are largely unknown. This study conducted a comparative analysis of the T. wilfordii transcriptome derived from its root and leaf tissues. Differential gene expression analysis identified a number of root-specific… More
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ARTICLE
Effect of UV Radiation and Other Abiotic Stress Factors on DNA of Different Wild Plant Species Grown in Three Successive Seasons in Alpine and Subalpine Regions
Svetla P. Gateva^1,*, Gabriele Jovtchev¹, Tsveta V. Angelova¹, Tzvetana P. Nonova², Nikolay Tyutyundzhiev³, Elena G. Geleva², Kostadin Katrandzhiev¹, Nina A. Nikolova², Dimitar Dimitrov⁴, Christo V. Angelov²
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 293-313, 2022, DOI:10.32604/phyton.2022.016397
（This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)
Abstract Plants in natural ecosystems are exposed to a combination of UV radiation, ionizing radiation (IR) and other abiotic factors. These factors change with the altitude. We investigated DNA alterations of some wild plants of different plant families in natural ecosystems at three altitudes in Rila Mountain, Bulgaria (1500, 1782, and 2925 m above sea level (a.s.l.) exposed to UV radiation, IR and other abiotic stresses, to assess the tolerance of plant species to the changing environmental conditions in three successive growth seasons. For this purpose, physicochemical, cytogenetic, and molecular methods were applied. DNA damage was assessed by micronucleus test and molecular… More
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ARTICLE
Genome-Wide Identification, Expression Profiling and Protein-Protein Interaction Properties of the BEL-Like Homeodomain Gene Family in Apple
Huifeng Li¹, Qiang Zhao², Hai Wang¹, Qinglong Dong^3,*, Yi Xu^4,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 315-331, 2022, DOI:10.32604/phyton.2022.016951
（This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)
Abstract BEL1-like homeodomain (BLH) family proteins are homeodomain transcription factors, which are found ubiquitously in plants and play important roles in regulating meristem and flower development. Although BLH proteins have been reported in some plant species, there is very little information available for plants in the Malus genus (e.g., apple tree:Malus domestica). In the present study, we identified 19 apple MdBLH genes. Phylogenetic analysis revealed that the MdBLH genes could be divided into five groups. Analysis of gene structure showed that MdBLH gene has four exons, and the third exon was 61 bp in length. Chromosomal location analysis suggested that the MdBLH… More
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ARTICLE
Light Intensity Affects the Coloration and Structure of Chimeric Leaves of Ananas comosus var. bracteatus
Wei Yang, Yuke Lin, Yanbin Xue, Meiqin Mao, Xuzixing Zhou, Hao Hu, Jiawen Liu, Lijun Feng, Huiling Zhang, Jiaheng Luo, Jun Ma^*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 333-348, 2022, DOI:10.32604/phyton.2022.016862
（This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)
Abstract Ananas comosus var. bracteatus is an important ornamental plant because of its green/white chimeric leaves. The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part. However, the red fades away in summer and winter. Light intensity is one of the most important factors affecting leaf color along the seasons. In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves, Ananas comosus var. bracteatus was grown under full sunlight, 50% shade and 75% shade for 75 days to evaluate the concentration of pigments, the color parameters (values L*,… More
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ARTICLE
Construction and Functional Analysis of CRISPR/Cas9 Vector of FAD2 Gene Family in Soybean
Zhifeng Xiao, Yukun Jin, Qi Zhang, Abraham Lamboro, Baozhu Dong, Zhuoyu Yang, Piwu Wang^*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 349-361, 2022, DOI:10.32604/phyton.2022.017451
（This article belongs to this Special Issue: Plant Physiology for Crop Production and Sustainable Agriculture)
Abstract Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. Functional analysis… More
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ARTICLE
Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize
Mengtong Liu¹, Yangyang Zhou¹, Tongyu Liu¹, Jianyu Lu¹, Jing Qu², Shuyan Guan^2,*, Yiyong Ma^2,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 363-374, 2022, DOI:10.32604/phyton.2022.016325
（This article belongs to this Special Issue: Plant Physiology for Crop Production and Sustainable Agriculture)
Abstract Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183… More
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ARTICLE
Transcriptomic Analysis of the Tolerance Response to Dehydration and Rehydration in Wheat Seedlings
Ping Zhang¹, Zhiyou Kong², Junna Liu¹, Yongjiang Liu¹, Qianchao Wang¹, Xiuju Huan¹, Li Li¹, Yunfeng Jiang³, Peng Qin^1,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 375-394, 2022, DOI:10.32604/phyton.2022.016358
（This article belongs to this Special Issue: Plant Physiology for Crop Production and Sustainable Agriculture)
Abstract Drought is the main abiotic stress that restricts wheat production. The rapid development of sequencing technology and its widespread application to various fields have revealed the structural characteristics and regulation of related genes through gene expression analysis. Here, we studied responses of wheat plants under drought and re-watering conditions, using morphological and physiological indicators. Moreover, a transcriptome analysis was conducted on Jingmai 12, a drought-resistant wheat strain, to explore the mechanism underlying the response of drought-resistant wheat seedlings to drought stress at the transcriptome level. Drought stress caused morphological and physiological changes in both drought-resistant and -sensitive varieties, but to… More
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ARTICLE
Effects of Nitrogen Exponential Fertilization on Growth and Nutrient Concentration of Hydrangea macrophylla Seedlings
Xueying Wang¹, Yonghong Hu^2,3, Fiza Liaquat⁴, Xianquan Zhang^2,3, Kang Ye^2,3, Jun Qin^2,3,*, Qunlu Liu^1,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 395-407, 2022, DOI:10.32604/phyton.2022.017981
（This article belongs to this Special Issue: The Effect of Soil Quality Degradation on the Plant Growth, Quality and Food Safety in Subtropical Agroforestry Ecosystems)
Abstract Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance. Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions. In this study, nitrogen requirements for producing Hydrangea macrophylla ‘Hanatemari’ that robust seedlings, nutrient dynamics, biomass and growth, was examined utilizing exponential fertilization. The potted seedlings were fertilized with urea under exponential regime at rates of 0.5, 1.5 and 2.0 g nitrogen/plant (EF1, EF2, and EF3), respectively. In addition, an unfertilized group treated with equal volume of deionized water was used as control.… More
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ARTICLE
Nitric Oxide Regulates Mitochondrial Fatty Acids and Promotes CBF Expression of Peach Fruit during Cold Storage
Xiaoshan Guo^1,2, Dandan Huang², Siyu Wen², Yang Bai², Shuhua Zhu^2,*, Jianrong Feng^1,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 409-427, 2022, DOI:10.32604/phyton.2022.018022
Abstract Low-temperature storage is convenient for postharvest preservation of peach fruit, but peach fruit is sensitive to cold damage, which lowers its quality. Nitric oxide (NO) has the potential to improve the bitter resistance of peach fruit. In this work, peach fruit was treated with 15 μmol L⁻¹ NO and 5 μmol L⁻¹ c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxo-3-oxide], to study changes in mitochondrial fatty acids and expression of the C-repeat binding factor (CBF). The results showed that 15 μmol L⁻¹ exogenous NO significantly maintained fruit quality, reduced peroxidation of mitochondrial fatty acids, increased the activities of the antioxidants superoxide dismutase (SOD), peroxidase (POD), catalase… More
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ARTICLE
Contrasting Characteristics of Lodging Resistance in Two Super-Rice Hybrids Differing in Harvest Index
Zui Tao¹, Tao Lei¹, Fangbo Cao¹, Jiana Chen¹, Xiaohong Yin^1,2, Tianfeng Liang^2,*, Min Huang^1,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 429-437, 2022, DOI:10.32604/phyton.2022.016955
Abstract The “super rice” breeding program in China has been successful in developing high-yielding hybrids, including few with high harvest index values. However, there is limited information on the relationship between lodging resistance and harvest index, and the mechanisms underlying the relationship in super-rice hybrids. In this study, a two-year field experiment was conducted to compare lodging resistance and its related traits between two super-rice hybrids differing in harvest index, i.e., Guiliangyou 2 (G2) with a high harvest index and Y-liangyou 1 (Y1) with a typical harvest index of modern high-yielding rice varieties. Results showed that compared to Y1, G2 was… More
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ARTICLE
Integrated Nutrient Management Improves Productivity and Quality of Sugarcane (Saccharum Officinarum L.)
Md. Shamsul Arefin¹, Md. Ariful Islam², Md. Mokhlesur Rahman³, Md. Abdul Alim⁴, Sabry Hassan⁵, Mahmoud F. K. Soliman⁶, Mohamed M. Hassan^5,*, Rajan Bhatt⁷, Akbar Hossain^8,*
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 439-469, 2022, DOI:10.32604/phyton.2022.017359
Abstract Sugarcane is one of the major important sugar yielding crops in Bangladesh. As an exhaustive crop, sugarcane removes a huge amount of plant nutrients from the soil. However, the combined use of organic and inorganic fertilizers can be a good approach to deal with nutrient depletion and promote sustainable crop production as well as improve soil health. Therefore, an attempt was made to identify the most fruitful and profitable integrated nutrient management on the aspects of growth, yield and quality of sugarcane in two consecutive growing seasons. Seven treatments: T₁=Control, T₂=165:55:120:30:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha⁻¹, T₃=Poultry Litter (PL) at 5 t ha⁻¹+95:51:87:9:10:2.5:4 kg… More
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ARTICLE
The Origin and Identity of the Calyculus in Loranthaceae: Inferred From the Floral Organogenesis of Loranthus tanakae Franch. & Sav.
Ruozhu Lin¹, Bei Cui^1,2 and Wenxia Zhao^1,*
Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 285-293, 2019, DOI:10.32604/phyton.2019.07182
Abstract The flowers of the hemiparasitic family Loranthaceae are always subtended by a rimmed structure known as the calyculus. The origin and identity of the calyculus have been disputed for more than a century. Various hypotheses have been proposed, for example, an outgrowth of the axis, a reduced calyx, and a bracteolar (prophyllar) origin, but controversies remain. To obtain a plausible explanation of the origin of the calyculus, we investigated the flowers of Loranthus tanakae using scanning electron microscopy and light microscopy to observe the entire developmental process of the floral parts. Our results show that bracts are not present in… More
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ARTICLE
Effects of Melatonin on Growth, Physiology and Gene Expression in Rice Seedlings Under Cadmium Stress
Xiachen Lv^1,#, Yunxia Fang^1,#, Lantian Zhang¹, Weiyi Zhang¹, Ling Xu¹, Jingjin Han¹, Bailing Jin², Xian Zhang¹, Xiaoqin Zhang^1,*, Dawei Xue^1,*
Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 91-100, 2019, DOI:10.32604/phyton.2019.06622
Abstract Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl₂ at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)-related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the application of exogenous… More
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ARTICLE
Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis
Quezada-Rivera JJ¹, RE Soria-Guerra², FS Pérez-Juárez², L Martínez-González², SE Valdés- Rodríguez³, NL Vasco-Méndez¹, JF Morales-Domínguez¹
Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 25-35, 2019, DOI:10.32604/phyton.2019.04549
Abstract The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified… More
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REVIEW
Plant Derived Antiviral Products for Potential Treatment of COVID-19: A Review
Rashid Iqbal Khan^1,*, Mazhar Abbas¹, Khurram Goraya², Muhammad Zafar-ul-Hye³, Subhan Danish³
Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 438-452, 2020, DOI:10.32604/phyton.2020.010972
Abstract COVID-19 caused by SARS-CoV-2 is declared global pandemic. The virus owing high resemblance with SARS-CoV and MERS-CoV has been placed in family of beta-coronavirus. However, transmission and infectivity rate of COVID-19 is quite higher as compared to other members of family. Effective management strategy with potential drug availability will break the virus transmission chain subsequently reduce the pressure on the healthcare system. Extensive research trials are underway to develop novel efficient therapeutics against SARS-CoV-2. In this review, we have discussed the origin and family of coronavirus, structure, genome and pathogenesis of virus SARS-CoV-2 inside human host cell; comparison among SARS,… More
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ARTICLE
Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate
S. J. Reyes-Zambrano^1,†, M. L. Ramírez-Merchant^1,†, C. Arias-Castro², M. A. Rodríguez-Mendiola², C. A. Lecona-Guzmán¹, V. M. Ruíz-Valdiviezo¹, D. González-Mendoza³, F. A. Gutiérrez-Miceli^1,*
Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504
Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with… More
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ARTICLE
Influence of Plant Growth Promoting Rhizobacterial Inoculation on Wheat Productivity Under Soil Salinity Stress
Muhammad Zafar-ul-Hye^1,*, Tariq Shahzad Bhutta¹, Muhammad Shaaban¹, Shahid Hussain¹, Muhammad Farooq Qayyum¹, Umar Aslam¹ and Zahir Ahmad Zahir²
Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 119-129, 2019, DOI:10.32604/phyton.2019.06570
Abstract Soil salinity affects the growth and yield of crops. The stress of soil salinity on plants can be mitigated by inoculation of plant growth promoting bacteria (PGPR). The influence of PGPR inoculation on wheat (Triticum aestivum L.) crop productivity under salinity stress has not been properly addressed so far. Therefore, the present study was conducted to investigate the effects of various PGPR strains (W14, W10 and 6K; alone and combined) at several growth attributes of wheat plant under different soil salinity gradients (3, 6 and 9 dS m-1). The growth attributes of wheat (height, roots, shoots, spikes, grains quality, biological… More
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